SYSTEMS AND METHODS FOR LIBRARY PREPARATION ADAPTERS

    公开(公告)号:US20240417718A1

    公开(公告)日:2024-12-19

    申请号:US18745437

    申请日:2024-06-17

    Abstract: Provided herein are systems, methods, compositions, and kits for library preparation. In some cases, multiple distinct types of adapter molecules may be provided to a template nucleic acid molecule. In some cases, a single type of adapter molecule may be provided to a template molecule. In some cases, multiple distinct types of adapter molecules may be sequentially provided to a template molecule to form multi-adapter template complexes.

    SYSTEMS AND METHODS FOR SEQUENCING WITH MULTI-PRIMING

    公开(公告)号:US20240401130A1

    公开(公告)日:2024-12-05

    申请号:US18738596

    申请日:2024-06-10

    Abstract: Provided herein are systems, methods, compositions, and kits for sequencing with multi-priming. In some cases, multiple distinct primer molecules may be provided to a template nucleic acid to hybridize to distinct regions of the template nucleic acid. In some cases, a connected primer molecule may be provided to a template nucleic acid to have multiple distinct primer regions hybridize to distinct regions of the template nucleic acid. Non-adjacent regions of the template nucleic acid may be sequenced in distinct sequencing operations.

    METHODS AND SYSTEMS FOR SUBSTRATE FUNCTIONALIZATION

    公开(公告)号:US20240326090A1

    公开(公告)日:2024-10-03

    申请号:US18608317

    申请日:2024-03-18

    CPC classification number: B05D1/60 B05D1/32

    Abstract: Provided herein are systems and methods for functionalizing a substrate. The method may comprise providing a substrate surface, wherein the substrate surface comprises a plurality of individually addressable locations, and exposing the substrate surface to a dose of organosilane vapor to generate a plurality of organosilane-functionalized locations on the substrate surface, wherein the plurality of organosilane-functionalized locations corresponds to at least a subset of the plurality of individually addressable locations. The dose of organosilane vapor may be provided at relatively low dose, in an example, at most about 1×1024 molecules per m2.

    METHODS FOR SEQUENCING NUCLEIC ACID MOLECULES WITH SEQUENTIAL BARCODES

    公开(公告)号:US20240309445A1

    公开(公告)日:2024-09-19

    申请号:US18281930

    申请日:2022-03-22

    CPC classification number: C12Q1/6874 C12N15/1065

    Abstract: Methods for determining a sequence of a polynucleotide comprising two or more barcode regions and an intervening region thereof are described herein. Flow sequencing methods can be used to sequence the barcode regions by extending a sequencing primer in a plurality of discrete flow steps, which include combining a primer/polynucleotide hybrid with a nucleotide that is incorporated into the extending primer if a complementary base in the poly nucleotide is present at the primer terminus. For one or more regions in the polynucleotide (e.g., barcode regions, a region of interest in the polynucleotide), the presence or absence of an incorporated nucleotide is detected (e.g., the sequence is determined for the said regions). For one or more regions in the polynucleotide (e.g., intervening regions), the primer can be extended without detecting the presence or absence of an incorporated nucleotide (e.g., the sequence is not determined), thereby increasing efficiency of the primer extension.

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