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21.发明授权公开(公告)号：US09862934B2
公开(公告)日：2018-01-09
申请号：US14670459
申请日：2015-03-27
申请人： GENETHON
IPC分类号： C12N15/866 , C12N15/86 , C12N7/00
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/14143 , C12N2710/14152 , C12N2750/14143 , C12N2800/50
摘要： The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with a reduced number of or without contaminating baculoviral virions.
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22.发明申请公开(公告)号：US20170152525A1
公开(公告)日：2017-06-01
申请号：US15424546
申请日：2017-02-03
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
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23.发明申请公开(公告)号：US20150307898A2
公开(公告)日：2015-10-29
申请号：US14603469
申请日：2015-01-23
申请人： UNIQURE IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
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24.发明授权Baculovirus-mediated transgene expression in both mammalian and insect cells 有权杆状病毒介导的哺乳动物和昆虫细胞中的转基因表达公开(公告)号：US08598136B2
公开(公告)日：2013-12-03
申请号：US13281810
申请日：2011-10-26
申请人： Yu-Chan Chao , Yueh-Lung Wu , Carol Pei Yin Wu , Chia-Hung Wang
发明人： Yu-Chan Chao , Yueh-Lung Wu , Carol Pei Yin Wu , Chia-Hung Wang
CPC分类号： C12N7/00 , C12N15/113 , C12N15/1137 , C12N15/67 , C12N15/86 , C12N2310/14 , C12N2710/14143 , C12N2710/14152 , C12N2799/027 , C12N2800/40 , C12N2830/50 , C12N2830/75 , C12N2830/85 , C12N2999/007
摘要： This invention relates to a method for expressing a gene in a cell. The method includes introducing into a cell an agent that inhibits one or both of miRNA pathway and antiviral defense pathway; and a baculovirus that contains a nucleic acid molecule including a gene operably linked to a promoter; and expressing the gene in the cell.
摘要翻译： 本发明涉及在细胞中表达基因的方法。 该方法包括向细胞中引入抑制miRNA途径和抗病毒防御途径中的一者或两者的药剂; 和包含核酸分子的杆状病毒，所述核酸分子包括可操作地连接于启动子的基因; 并在细胞中表达该基因。
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25.发明申请公开(公告)号：US20130296532A1
公开(公告)日：2013-11-07
申请号：US13945505
申请日：2013-07-18
申请人： uniQure IP B.V.
发明人： Wilhelmus Theodorus Johannes Maria Christiaan HERMENS , Saskia Jacoba Petronella HAAST , Dennis Johan BIESMANS , Andrew Christian BAKKER
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
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26.发明授权Vectors with modified initiation codon for the translation of AAV-Rep78 useful for production of AAV 有权具有用于AAV-Rep78翻译的修饰起始密码子的载体可用于生产AAV公开(公告)号：US08512981B2
公开(公告)日：2013-08-20
申请号：US12306239
申请日：2007-06-20
申请人： Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
发明人： Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2710/14043 , C12N2710/14044 , C12N2710/14143 , C12N2710/14144 , C12N2710/14152 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50
摘要： The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
摘要翻译： 本发明涉及用于在昆虫细胞中生产重组细小病毒（例如腺相关病毒）载体的核酸构建体，包含这种构建体的昆虫细胞以及其中细胞用于产生重组细小病毒病毒粒子的方法。 昆虫细胞优选包含编码细小病毒rep蛋白的第一核苷酸序列，由此翻译细小病毒Rep78蛋白的起始密码子是次要起始密码子，其在昆虫细胞中表达时会影响部分外显子跳跃。 昆虫细胞还包含包含至少一个细小病毒（AAV）倒置末端重复（ITR）核苷酸序列的第二核苷酸序列和包含编码细小病毒衣壳蛋白的序列的第三核苷酸序列。
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