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    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS
    33.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS 有权
    用于生产对苯二酸和其他化合物的微生物

    公开(公告)号：US20120115194A1

    公开(公告)日：2012-05-10

    申请号：US13288699

    申请日：2011-11-03

    Applicant: Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    IPC: C12P7/44 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

    Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

    Abstract translation: 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。

    METHODS FOR INCREASING PRODUCT YIELDS
    35.
    发明申请
    METHODS FOR INCREASING PRODUCT YIELDS 有权
    增加产品线的方法

    公开(公告)号：US20110201089A1

    公开(公告)日：2011-08-18

    申请号：US13011796

    申请日：2011-01-21

    Applicant: Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    Inventor： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC: C12N1/00

    CPC classification number: C12P7/00 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0095 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/42 ,  C12P19/42

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    Abstract translation: 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间段内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为结构单元的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    36.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE 有权
    生产环己酮的有机体

    公开(公告)号：US20110014668A1

    公开(公告)日：2011-01-20

    申请号：US12780802

    申请日：2010-05-14

    Applicant: Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    IPC: C12P7/26 ,  C12N1/00

    CPC classification number: C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

    Abstract translation: 非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。 途径包括2-酮环己烷-1-羧基-CoA水解酶（作用于C-C键），2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶的酶（作用 在硫酯上），2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。 途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶（作用于C-C键）的酶，6-酮环己-1-烯-1-羧基-CoA合成酶，6- 酮酮环己-1-烯-1-羧基-CoA水解酶（作用于硫酯），6-酮环己-1-烯-1-羧基-CoA转移酶，6-酮环己-1-烯-1-羧基-CoA还原酶， 6-酮环己-1-烯-1-羧酸脱羧酶，6-酮环己-1-烯-1-羧酸还原酶，2-酮环己烷-1-羧基-CoA合成酶，2-酮环己烷-1-羧基-CoA 转移酶，2-酮环己烷-1-羧基-CoA水解酶（作用于硫酯），2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。 途径包括己二酸半醛脱水酶，环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。 途径包括3-氧代邻苯二甲酸脱羧酶，4-乙酰基丁酸脱水酶，3-羟基环己酮脱氢酶，2-环己烯酮水合酶，环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶，3-氧代木糖酰-CoA水解酶 （作用于硫酯）和3-氧代木糖酰辅酶A转移酶。 每个这些途径可以包括PEP羧基激酶。 生产环己酮的方法包括培养这些非天然存在的微生物。

    Method for redesign of microbial production systems
    37.
    发明授权
    Method for redesign of microbial production systems 有权
    微生物生产系统重新设计方法

    公开(公告)号：US07826975B2

    公开(公告)日：2010-11-02

    申请号：US10929091

    申请日：2004-08-26

    Applicant: Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    Inventor： Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    IPC: G06F19/00

    CPC classification number: G01N33/5005 ,  G01N33/54366 ,  G06F19/12 ,  G06F19/18

    Abstract: A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

    Abstract translation: 用于鉴定添加到生物体特异性代谢网络以实现宿主期望生物转化的功能的计算机辅助方法包括从通用数据库获得反应以提供化学计量平衡，至少部分地基于化学计量平衡路径， 反应和底物以使生产宿主中的非天然功能数量最小化，并将至少一种化学计量平衡的途径并入宿主以提供所需的生物转化。 可以存储经修改的代谢网络的表示。

    Microorganisms for the production of adipic acid and other compounds
    38.
    发明授权
    Microorganisms for the production of adipic acid and other compounds 有权
    用于生产己二酸等化合物的微生物

    公开(公告)号：US07799545B2

    公开(公告)日：2010-09-21

    申请号：US12413355

    申请日：2009-03-27

    Applicant: Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    IPC: C12P7/40 ,  C12N1/20

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

    Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

    Abstract translation: 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。

    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL
    39.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL 审中-公开
    用于生产甲基乙基酮和2-丁醇的微生物

    公开(公告)号：US20100184173A1

    公开(公告)日：2010-07-22

    申请号：US12618545

    申请日：2009-11-13

    Applicant: Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    Inventor： Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    IPC: C12P7/26 ,  C12N1/00 ,  C12P7/16

    CPC classification number: C12P7/16 ,  C12N15/52 ,  C12P7/26 ,  Y02E50/10

    Abstract: A non-naturally occurring microbial organism having a methyl ethyl ketone pathway includes at least one exogenous nucleic acid encoding a methyl ethyl ketone pathway enzyme expressed in a sufficient amount to produce methyl ethyl ketone. The methyl ethyl ketone pathway includes a β-ketothiolase, a β-ketovalerate decarboxylase and an enzyme selected from the group consisting of a β-ketovaleryl-CoA hydrolase and a β-ketovaleryl-CoA transferase. Alternatively, the methyl ethyl ketone pathway includes a 2-methylacetoacetyl-CoA thiolase, a 2-methylacetoacetate decarboxylase and an enzyme selected from the group consisting of a 2-methylacetoacetyl-CoA hydrolase and a 2-methylacetoacetyl-CoA transferase. Either pathway can further include a methyl ethyl ketone reductase to produce 2-BuOH. A method for producing methyl ethyl ketone or 2-BuOH includes culturing these non-naturally occurring microbial organisms under conditions, and for a sufficient period of time, to produce methyl ethyl ketone or 2-BuOH.

    Abstract translation: 具有甲基乙基酮途径的非天然存在的微生物包括至少一种编码甲基乙基酮途径酶的外源核酸，其以足以产生甲基乙基酮的量表达。 甲基乙基酮途径包括α-酮硫解酶，α-酮戊二酸脱羧酶和选自α-酮戊酰基-CoA水解酶和一种α-酮戊酰基-CoA转移酶的酶。 或者，甲基乙基酮途径包括2-甲基乙酰乙酰辅酶A硫解酶，2-甲基乙酰乙酸脱羧酶和选自2-甲基乙酰乙酰辅酶A水解酶和2-甲基乙酰乙酰辅酶A转移酶的酶。 任一途径还可以包括甲基乙基酮还原酶以产生2-BuOH。 一种生产甲基乙基酮或2-BuOH的方法包括在条件和足够的时间内培养这些非天然存在的微生物生产甲基乙基酮或2-BuOH。

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