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    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION COMPRISING THE USE OF AN ALPHA-1,2-MANNOSIDASE
    31.
    发明申请
    PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION COMPRISING THE USE OF AN ALPHA-1,2-MANNOSIDASE 审中-公开
    生产含有ALPHA-1,2-MANNOSIDASE的使用的减少的O-糖苷酸的甘氨酸

    公开(公告)号：US20120135461A1

    公开(公告)日：2012-05-31

    申请号：US13191811

    申请日：2011-07-27

    Applicant: WILLIAM JAMES COOK ,  STEFAN WILDT

    Inventor： WILLIAM JAMES COOK ,  STEFAN WILDT

    IPC: C12P21/00

    CPC classification number: C12P21/005 ,  C07K16/00 ,  C07K2317/41 ,  C12N9/2488 ,  C12N15/815 ,  C12N15/8257 ,  C12Y302/01113

    Abstract: A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of one or more α-1,2-mannosidases from Coccidiodes immitis, Coccidiodes posadasii, Penicillium citrinum, Magnaporthe grisea, Aspergillus saitoi, Aspergillus oryzae, and Chaetomiun globosum or a catalytically active fragment of said α-1,2-mannosidase.

    Abstract translation: 描述了一种用于生产具有减少量的O-连接糖基化的蛋白质组合物的方法。 该方法包括在存在一种或多种来自椰子球菌，椰子球菌，青霉菌，稻瘟病菌，沙司酸曲霉，米曲霉和球孢白僵菌的一种或多种α-1,2-甘露糖苷酶的存在下培养的细胞中产生蛋白质，或催化活性片段 的所述α-1,2-甘露糖苷酶。

    Immunoglobulins comprising predominantly a glcnacman3glcnac2 glycoform
    36.
    发明申请
    Immunoglobulins comprising predominantly a glcnacman3glcnac2 glycoform 审中-公开
    主要包含glcnacman3glcnac2糖型的免疫球蛋白

    公开(公告)号：US20090226464A1

    公开(公告)日：2009-09-10

    申请号：US11990588

    申请日：2005-09-09

    Applicant: Tillman Gerngross ,  Stefan Wildt ,  Huijuan Li

    Inventor： Tillman Gerngross ,  Stefan Wildt ,  Huijuan Li

    IPC: A61K39/395 ,  C12N5/06 ,  C12P21/08

    CPC classification number: C07K16/2887 ,  C07K16/00 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/72

    Abstract: The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is selected from the group consisting of Man7GlcNAc2 and Man8GlcNAc2.

    Abstract translation: 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及包含具有特定富含N-聚糖结构的抗体的药物组合物，其中所述N-聚糖结构选自Man7GlcNAc2和Man8GlcNAc2。

    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
    39.
    发明授权
    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权
    用于在低等真核生物中生产修饰的N-聚糖的组合DNA文库

    公开(公告)号：US07449308B2

    公开(公告)日：2008-11-11

    申请号：US10371877

    申请日：2003-02-20

    Applicant: Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    Inventor： Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC: C12N15/00

    CPC classification number: C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

    Abstract translation: 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化的宿主细胞中制备的N-聚糖具有可以通过异源表达一种或多种酶（例如糖基转移酶）而进一步修饰的Man III / 糖转运蛋白和甘露糖苷酶，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

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