公开(公告)号：US20210129148A1

公开(公告)日：2021-05-06

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin NESS ,  Debkishore MITRA ,  Donald A. MASQUELIER ,  Anthony MAKAREWICZ ,  Christopher HINDSON ,  Benjamin HINDSON ,  Serge SAXONOV

IPC: B01L3/00 ,  B01L3/02

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20210079466A1

公开(公告)日：2021-03-18

申请号：US17033218

申请日：2020-09-25

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Serge SAXONOV

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12P19/34 ,  C12N15/10

Abstract: Provided herein are methods and systems for processing a nucleic acid molecule from a biological particle (e.g., cell). A plurality of partitions (e.g., droplets) may be generated such that partitions of the plurality of partitions each include a biological particle (e.g., cell) comprising the nucleic acid molecule and a particle (e.g., bead). The partitions can be processed (e.g., imaged) to obtain one or more physical and/or optical properties of their respective biological particles. The nucleic acid molecules included in the partitions can be barcoded and sequenced (e.g., using nucleic acid barcode molecules coupled to the particles of the partitions) to generate nucleic acid sequences of the nucleic acid molecules. The nucleic acid sequences can be electronically associated with the one or more optical properties of the biological particles.

公开(公告)号：US20210053053A1

公开(公告)日：2021-02-25

申请号：US16998832

申请日：2020-08-20

Applicant: 10X Genomics, Inc.

Inventor： Alireza SALMANZADEH ,  Rajiv BHARADWAJ

IPC: B01L3/00

Abstract: Devices and systems employing surface acoustic waves and their methods of use, for detecting the contents of and mixing fluids are provided. Devices and systems of the invention include a piezoelectric layer on an elastic base layer and a fluidic layer including a channel.

公开(公告)号：US20200230606A1

公开(公告)日：2020-07-23

申请号：US16840619

申请日：2020-04-06

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  G01N33/58 ,  C12Q1/6806 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01F5/04 ,  B01F11/02 ,  G01N33/543

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190351417A1

公开(公告)日：2019-11-21

申请号：US16443629

申请日：2019-06-17

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F3/08 ,  G01N33/58 ,  B01L3/02 ,  B01F13/00 ,  C12Q1/6806 ,  G01N33/543 ,  B01F5/04 ,  B01F11/02

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190153532A1

公开(公告)日：2019-05-23

申请号：US16228261

申请日：2018-12-20

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Serge SAXONOV

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1089 ,  C12N15/1093 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2563/179 ,  C12Q2563/159

Abstract: Provided herein are methods and systems for processing a nucleic acid molecule from a biological particle (e.g., cell). A plurality of partitions (e.g., droplets) may be generated such that partitions of the plurality of partitions each include a biological particle (e.g., cell) comprising the nucleic acid molecule and a particle (e.g., bead). The partitions can be processed (e.g., imaged) to obtain one or more physical and/or optical properties of their respective biological particles. The nucleic acid molecules included in the partitions can be barcoded and sequenced (e.g., using nucleic acid barcode molecules coupled to the particles of the partitions) to generate nucleic acid sequences of the nucleic acid molecules. The nucleic acid sequences can be electronically associated with the one or more optical properties of the biological particles.

公开(公告)号：US20190064173A1

公开(公告)日：2019-02-28

申请号：US15977875

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: G01N33/58 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190060904A1

公开(公告)日：2019-02-28

申请号：US15977805

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F13/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190060890A1

公开(公告)日：2019-02-28

申请号：US15977812

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/02 ,  B01L3/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.