公开(公告)号：US20070087400A1

公开(公告)日：2007-04-19

申请号：US11509796

申请日：2006-08-24

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

IPC分类号： C12Q1/26 ,  C12P21/06 ,  C07H21/04 ,  C12N9/16 ,  C12N9/88 ,  C12N1/21

CPC分类号： C12Q1/34 ,  B82Y30/00

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08921620B2

公开(公告)日：2014-12-30

申请号：US13450217

申请日：2012-04-18

申请人： Keith V. Wood ,  Dieter Klaubert ,  Georgyi V. Los ,  Robert F. Bulleit ,  Mark McDougall ,  Chad Zimprich

发明人： Keith V. Wood ,  Dieter Klaubert ,  Georgyi V. Los ,  Robert F. Bulleit ,  Mark McDougall ,  Chad Zimprich

IPC分类号： C07C19/00 ,  C07C21/00 ,  G01N33/53 ,  C12Q1/34 ,  C12N9/14

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20120330001A1

公开(公告)日：2012-12-27

申请号：US13477593

申请日：2012-05-22

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C07C235/08 ,  C07C19/075 ,  C08B1/00 ,  C07C19/01

CPC分类号： C12N9/14 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K2299/00 ,  C12N9/0069 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/66 ,  C12Y113/12005 ,  C12Y308/01005

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08257939B2

公开(公告)日：2012-09-04

申请号：US12975020

申请日：2010-12-21

申请人： Keith V. Wood ,  Georgyi V. Los ,  Robert F. Bulleit ,  Dieter Klaubert ,  Mark McDougall ,  Chad Zimprich

发明人： Keith V. Wood ,  Georgyi V. Los ,  Robert F. Bulleit ,  Dieter Klaubert ,  Mark McDougall ,  Chad Zimprich

IPC分类号： G01N33/53 ,  C12Q1/34 ,  C12N9/14 ,  C12P21/02 ,  C07C19/00 ,  C07C21/00

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20110207195A1

公开(公告)日：2011-08-25

申请号：US13021295

申请日：2011-02-04

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12N11/00

CPC分类号： C12N9/14 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K2299/00 ,  C12N9/0069 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/66 ,  C12Y113/12005 ,  C12Y308/01005

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20110171673A1

公开(公告)日：2011-07-14

申请号：US13046297

申请日：2011-03-11

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12Q1/34

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US07935803B2

公开(公告)日：2011-05-03

申请号：US12075160

申请日：2008-03-10

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C07H21/00 ,  C12N9/14 ,  C12P21/02 ,  C12N1/21 ,  C07C17/23

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20110053162A1

公开(公告)日：2011-03-03

申请号：US12872467

申请日：2010-08-31

申请人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

发明人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

IPC分类号： C12Q1/68 ,  C07D451/00 ,  C07F9/06 ,  C12Q1/02 ,  G01N33/00

CPC分类号： G01N33/582 ,  A61K49/0032 ,  A61K49/0056 ,  C09B23/0008 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083

摘要： The invention provides compounds and compositions of Formulas I-VII, and methods of using the compounds. The compounds can be used to prepare dye conjugates that are uniformly and substantially more fluorescent on proteins, nucleic acids or other biopolymers, than conjugates labeled with structurally similar known carbocyanine dyes. In addition to having more intense fluorescence emission than structurally similar dyes at virtually identical wavelengths, and decreased artifacts in their absorption spectra upon conjugation to biopolymers, the compounds can have greater photostability and/or higher absorbance (extinction coefficients) at the wavelength(s) of peak absorbance than such structurally similar dyes.

摘要翻译： 本发明提供式I-VII的化合物和组合物以及使用该化合物的方法。 该化合物可用于制备在蛋白质，核酸或其他生物聚合物上均匀且基本上更多荧光的染料缀合物，而不是与结构上相似的已知碳菁染料标记的缀合物。 除了与实际相同的波长下的结构相似的染料具有更强烈的荧光发射以及在与生物聚合物共轭时其吸收光谱中的伪影减少，化合物在波长处具有更大的光稳定性和/或更高的吸光度（消光系数） 的吸光度比这种结构相似的染料高。

公开(公告)号：US20080274488A1

公开(公告)日：2008-11-06

申请号：US12075160

申请日：2008-03-10

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12Q1/34 ,  C12N15/11 ,  C12P21/04

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.