公开(公告)号：US10094809B2

公开(公告)日：2018-10-09

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N30/72 ,  G01N33/74 ,  H01J49/16 ,  H01J49/00

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US20170052180A1

公开(公告)日：2017-02-23

申请号：US15118850

申请日：2015-02-25

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  G01N30/88 ,  C12Q1/04 ,  G01N30/72 ,  C12Q1/68 ,  G01N33/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to select a peptide labeled with a first tag of a known microbe, fragment the labeled peptide of the known microbe, and monitor for an intensity of the first tag in an MRM method using a processor. An ion source provides a beam of ions from a sample that includes peptides labeled with the first tag. The first tag binds to a peptide of a known microbe and is cleaved from the peptide of the known microbe during mass spectrometry. The mass spectrometer receives the beam of ions and is adapted to perform the MRM method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample using the processor.

Abstract translation: 提供了使用可切割标签进行微生物鉴定的系统和方法。 将控制信息发送到质谱仪以选择用已知微生物的第一标签标记的肽，对已知微生物的标记肽进行片段化，并使用处理器以MRM方法监测第一标签的强度。 离子源提供来自样品的离子束，其包括用第一标签标记的肽。 第一个标签结合已知微生物的肽，并且在质谱期间从已知微生物的肽切割。 质谱仪接收离子束，适用于在离子束上执行MRM方法。 如果从质谱仪接收的第一标签的强度超过阈值，则使用处理器在样品中识别已知的微生物。

公开(公告)号：US20150051111A1

公开(公告)日：2015-02-19

申请号：US14367230

申请日：2013-01-16

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Subhasish Purkayastha

IPC: G01N33/74 ,  G01N33/58

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2560/00 ,  Y10T436/18 ,  Y10T436/200833 ,  Y10T436/203332

Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.

Abstract translation: 描述了使用季氨氧基Cookson（QAOC）试剂对包含酚类OH的样品（例如包含酚类OH）的类固醇进行质谱分析的方法。 QAOC试剂可以改善酚类OH样品的电离和断裂性能，从而提高定量和鉴定。 该方法可以包括用QAOC试剂衍生酚OH样品以产生加合物，并使用质谱分析加合物。 样品的衍生化可以是一步反应，其中QAOC试剂包含氨氧基MS标签或可以是多步反应，其中加合物通过羰基取代的基于PTAD的试剂与样品的反应形成，然后与 氨氧基MS标签。 在将样品与试剂反应之前也可以富集样品。 该方法还可以允许复用。