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    Microbial identification and quantitation using MS cleavable tags
    2.
    发明授权
    Microbial identification and quantitation using MS cleavable tags 有权

    公开(公告)号：US11549944B2

    公开(公告)日：2023-01-10

    申请号：US16358533

    申请日：2019-03-19

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Subhasish Purkayastha ,  Subhakar Dey

    IPC: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/88 ,  G01N33/68

    Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

    INTEGRATED SAMPLE PROCESSING SYSTEM WITH VARIABLE WORKFLOWS
    3.
    发明申请
    INTEGRATED SAMPLE PROCESSING SYSTEM WITH VARIABLE WORKFLOWS 有权

    公开(公告)号：US20210003551A1

    公开(公告)日：2021-01-07

    申请号：US16956405

    申请日：2018-12-19

    Applicant: Beckman Coulter, Inc. ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Aaron Hudson ,  Takayuki Mizutani ,  Subhasish Purkayastha ,  Thomas W. Roscoe

    IPC: G01N33/487 ,  G01N35/00

    Abstract: One embodiment of the invention is directed to a sample processing system for analyzing a biological sample from a patient. The sample processing system comprises: a plurality of analyzers comprising at least one mass spectrometer, wherein each analyzer in the plurality of analyzers is configured to acquire at least one measurement value corresponding to at least one characteristic of the biological sample; at least one data storage component which stores (i) a list of parameters for the plurality of analyzers, and (ii) at least two condition sets, which contain data associated with completing one or more test orders. The condition sets contain data which differ by at least one variable; and a control system operatively coupled to the plurality of analyzers, and the at least one data storage component. The control system is configured to (i) determine which condition set of the at least two condition sets to use based on the determined condition set, (ii) determine which analyzer or analyzers of the plurality of analyzers to use to process each test order based on the determined condition set and one or more parameters from the list of parameters, and (iii) cause the determined analyzer or analyzers to acquire one or more measurement values for the biological sample.

    MASS SPECTROMETRY QUANTITATION OF P450 PROTEIN ISOFORMS IN HEPATOCYTES
    5.
    发明申请
    MASS SPECTROMETRY QUANTITATION OF P450 PROTEIN ISOFORMS IN HEPATOCYTES 审中-公开
    P450蛋白质蛋白质在质谱中的质谱测定

    公开(公告)号：US20150045256A1

    公开(公告)日：2015-02-12

    申请号：US14465460

    申请日：2014-08-21

    Applicant: DH Technologies Development Pte. Ltd.

    Inventor： Brian L. Williamson ,  Subhasish Purkayastha

    IPC: G01N33/68 ,  G01N33/50

    CPC classification number: C12Q1/26 ,  C12N9/0077 ,  G01N30/7233 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/5067 ,  G01N33/6848 ,  G01N2030/8831 ,  G01N2333/90245 ,  G01N2500/04 ,  G01N2500/10 ,  G01N2500/20 ,  G01N2560/00 ,  Y10T436/24

    Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.

    Abstract translation: 提供了用于筛选细胞色素P450（CYP）诱导的药物的方法，并且可以包括将药物与含有微粒体的生物样品孵育，然后定量至少一种细胞色素P450同种型。 同种型可以选自2B6,3A4,1A2和3A5同种型。 在一些实施方案中，该方法使用液相色谱串联质谱（LC-MSMS）。 可以将定量值与阈值进行比较，并且当定量值不超过阈值时，可以确定药物表现出可接受的CYP诱导电位。 还提供了分离的肽。

    SCOUT MRM FOR SCREENING AND DIAGNOSTIC ASSAYS
    7.
    发明公开
    SCOUT MRM FOR SCREENING AND DIAGNOSTIC ASSAYS 审中-公开

    公开(公告)号：US20240345043A1

    公开(公告)日：2024-10-17

    申请号：US18577407

    申请日：2022-07-05

    Applicant: DH Technologies Development Pte. Ltd.

    Inventor： Subhasish Purkayastha ,  Yves Le Blanc

    IPC: G01N30/72 ,  B01D15/08 ,  G01N30/86 ,  H01J49/00

    CPC classification number: G01N30/7233 ,  B01D15/08 ,  G01N30/8682 ,  H01J49/004

    Abstract: One or more known compounds are separated from a mixture using a separation device that allows processor-controlled adjustment of a separation parameter. The separated compounds are ionized and, for each cycle of a plurality of cycles, a mass spectrometer executes on the ion beam a series of MRM transitions read from a list. Two or more contiguous groups of MRM transitions to be monitored separately are received. Each group includes at least one sentinel transition that identifies a next group that is to be monitored and identifies a value for the separation parameter for the next group. A first group is placed on the list. When a sentinel transition of the first group is detected, a next group identified by the sentinel transition is placed on the list and the separation parameter is adjusted to a value identified by the sentinel transition for the next group.

    MULTIPLEXED EXTERNAL CALIBRATOR AND CONTROL FOR SCREENING AND DIAGNOSTIC ASSAYS
    8.
    发明申请
    MULTIPLEXED EXTERNAL CALIBRATOR AND CONTROL FOR SCREENING AND DIAGNOSTIC ASSAYS 有权

    公开(公告)号：US20220308066A1

    公开(公告)日：2022-09-29

    申请号：US17419844

    申请日：2019-10-16

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Jason Cournoyer ,  Subhakar Dey ,  Amy E. Trudel ,  Subhasish Purkayastha

    IPC: G01N33/68 ,  G01N33/74

    Abstract: Methods, compositions, kits, and apparatuses for MS-based quantification of a target analyte (e.g., a peptide, a hormone) in a sample are provided for increasing the productivity and/or throughput of samples by reducing the time and/or cost associated with conventional techniques for external instrument calibration that typically utilize a series of calibrators that are sequentially run through the same analytical process as a batch of samples. In various aspects, calibration mixtures are provided containing a known quantity of a plurality of calibrants for a target analyte of interest, with each calibrant being distinguishable in the calibration mixture by mass spectrometry such that a complete calibration curve can be generated from a single external calibration run.

    Magnetic Elements for Processing Fluids
    9.
    发明申请
    Magnetic Elements for Processing Fluids 审中-公开
    加工液磁性元件

    公开(公告)号：US20170074871A1

    公开(公告)日：2017-03-16

    申请号：US15121803

    申请日：2015-02-27

    Applicant: DH Technologies Development PTE Ltd.

    Inventor： John L. Campbell ,  Thomas R. Covey ,  Chang Liu ,  Subhasish Purkayastha

    IPC: G01N33/543 ,  B01F13/08 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38

    CPC classification number: G01N33/54326 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N1/38 ,  G01N27/745 ,  G01N35/0098 ,  G01N2001/386

    Abstract: Methods and apparatus for processing fluids on a macro- or micro-scale are described. In various aspects, a fluid may have a plurality of elongated (i.e., substantially rod-shaped) magnetic elements disposed therein within a fluid container. An illustrative fluid container is an actuator electrode or a processing vial of a microfluidic device, such as a digital microfluidic device. A magnet component may be configured to generate a magnetic force sufficient to influence the movement of the plurality of elongated magnetic elements within the fluid to be processed. For example, the magnetic force (or magnetic force gradient) may influence the plurality of elongated magnetic elements to rotate, spin, and/or move laterally side-to-side. The shape and movements of the plurality of elongated magnetic elements facilitate the rapid and efficient processing of the fluid, such as fluid mixing and/or fluid separation.

    Abstract translation: 描述了在宏观或微观尺度上处理流体的方法和装置。 在各个方面，流体可以具有设置在流体容器内的多个细长（即基本棒状）的磁性元件。 说明性的流体容器是诸如数字微流体装置的微流体装置的致动器电极或处理小瓶。 磁体部件可以被配置为产生足以影响待处理流体中的多个细长磁性元件的运动的磁力。 例如，磁力（或磁力梯度）可以影响多个细长的磁性元件以使它们在侧面上相互旋转，旋转和/或横向移动。 多个细长磁性元件的形状和运动有助于流体的快速和有效的处理，例如流体混合和/或流体分离。

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