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公开(公告)号：US10094809B2
公开(公告)日：2018-10-09
申请号：US15339119
申请日：2016-10-31
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Subhakar Dey , Sasi Pillai , Brian L. Williamson , Subhasish Purkayastha , Michal Weinstock
Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
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2.发明申请公开(公告)号：US20170115258A1
公开(公告)日：2017-04-27
申请号：US15339119
申请日：2016-10-31
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Subhakar Dey , Sasi Pillai , Brian L. Williamson , Subhasish Purkayastha , Michal Weinstock
CPC classification number: G01N30/72 , G01N27/62 , G01N33/64 , G01N33/743 , G01N2030/045 , G01N2030/884 , H01J49/00 , H01J49/0045 , H01J49/164 , Y10T436/147777 , Y10T436/200833
Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
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3.发明授权Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow 有权使用试剂化合物，标记策略和质谱工作流程对酮和醛分析物进行具体分析公开(公告)号：US09500622B2
公开(公告)日：2016-11-22
申请号：US13897628
申请日：2013-05-20
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Subhakar Dey , Sasi Pillal , Brian L. Williamson , Subhasish Purkayastha , Michal Weinstock
CPC classification number: G01N30/72 , G01N27/62 , G01N33/64 , G01N33/743 , G01N2030/045 , G01N2030/884 , H01J49/00 , H01J49/0045 , H01J49/164 , Y10T436/147777 , Y10T436/200833
Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。
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4.发明授权公开(公告)号：US09188596B2
公开(公告)日：2015-11-17
申请号：US14104117
申请日：2013-12-12
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Subhakar Dey , Sasi Pillal , Brian L. Williamson , Subhasish Purkayastha
CPC classification number: G01N33/82 , Y10T436/20 , Y10T436/200833 , Y10T436/203332 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/25875
Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.
Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。
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Patent Agency Ranking
5.发明申请QUANTITATIVE ANALYSIS OF VITAMIN D3, VITAMIN D2, AND METABOLITES THEREOF 审中-公开维生素D3，维生素D2及其代谢物的定量分析公开(公告)号：US20140127825A1
公开(公告)日：2014-05-08
申请号：US14104117
申请日：2013-12-12
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Subhakar Dey , Sasi Pillal , Brian L. Williamson , Subhasish Purkayastha
IPC: G01N33/82
CPC classification number: G01N33/82 , Y10T436/20 , Y10T436/200833 , Y10T436/203332 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/25875
Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.
Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。
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6.发明申请公开(公告)号：US20150045256A1
公开(公告)日：2015-02-12
申请号：US14465460
申请日：2014-08-21
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Brian L. Williamson , Subhasish Purkayastha
CPC classification number: C12Q1/26 , C12N9/0077 , G01N30/7233 , G01N33/502 , G01N33/5023 , G01N33/5067 , G01N33/6848 , G01N2030/8831 , G01N2333/90245 , G01N2500/04 , G01N2500/10 , G01N2500/20 , G01N2560/00 , Y10T436/24
Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.
Abstract translation: 提供了用于筛选细胞色素P450（CYP）诱导的药物的方法，并且可以包括将药物与含有微粒体的生物样品孵育，然后定量至少一种细胞色素P450同种型。 同种型可以选自2B6,3A4,1A2和3A5同种型。 在一些实施方案中，该方法使用液相色谱串联质谱（LC-MSMS）。 可以将定量值与阈值进行比较，并且当定量值不超过阈值时，可以确定药物表现出可接受的CYP诱导电位。 还提供了分离的肽。
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7.发明申请SPECIFIC ANALYSIS OF KETONE AND ALDEHYDE ANALYTES USING REAGENT COMPOUNDS, LABELING STRATEGIES, AND MASS SPECTROMETRY WORKFLOW 审中-公开使用试剂化合物，标记策略和大量光谱仪工作流程的酮和醛分析仪的具体分析公开(公告)号：US20140004616A1
公开(公告)日：2014-01-02
申请号：US13897628
申请日：2013-05-20
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Subhakar Dey , Sasi Pillal , Brian L. Williamson , Subhasish Purkayastha , Michal Weinstock
IPC: G01N27/62
CPC classification number: G01N30/72 , G01N27/62 , G01N33/64 , G01N33/743 , G01N2030/045 , G01N2030/884 , H01J49/00 , H01J49/0045 , H01J49/164 , Y10T436/147777 , Y10T436/200833
Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。
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公开(公告)号：US09546393B2
公开(公告)日：2017-01-17
申请号：US14602917
申请日：2015-01-22
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Brian L. Williamson , Subhasish Purkayastha
CPC classification number: C12Q1/26 , C12N9/0077 , G01N30/7233 , G01N33/502 , G01N33/5023 , G01N33/5067 , G01N33/6848 , G01N2030/8831 , G01N2333/90245 , G01N2500/04 , G01N2500/10 , G01N2500/20 , G01N2560/00 , Y10T436/24
Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.
Abstract translation: 提供了用于筛选细胞色素P450（CYP）诱导的药物的方法，并且可以包括将药物与含有微粒体的生物样品孵育，然后定量至少一种细胞色素P450同种型。 同种型可以选自2B6,3A4,1A2和3A5同种型。 在一些实施方案中，该方法使用液相色谱串联质谱（LC-MSMS）。 可以将定量值与阈值进行比较，并且当定量值不超过阈值时，可以确定药物表现出可接受的CYP诱导电位。 还提供了分离的肽。
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9.发明申请MASS SPECTROMETRY QUANTITATION OF P450 ISOFORMS IN HEPATOCYTES 审中-公开P450 ISOFORMS在HEPATOCYTES中的质谱分析公开(公告)号：US20150140600A1
公开(公告)日：2015-05-21
申请号：US14602917
申请日：2015-01-22
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Brian L. Williamson , Subhasish Purkayastha
CPC classification number: C12Q1/26 , C12N9/0077 , G01N30/7233 , G01N33/502 , G01N33/5023 , G01N33/5067 , G01N33/6848 , G01N2030/8831 , G01N2333/90245 , G01N2500/04 , G01N2500/10 , G01N2500/20 , G01N2560/00 , Y10T436/24
Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.
Abstract translation: 提供了用于筛选细胞色素P450（CYP）诱导的药物的方法，并且可以包括将药物与含有微粒体的生物样品孵育，然后定量至少一种细胞色素P450同种型。 同种型可以选自2B6,3A4,1A2和3A5同种型。 在一些实施方案中，该方法使用液相色谱串联质谱（LC-MSMS）。 可以将定量值与阈值进行比较，并且当定量值不超过阈值时，可以确定药物表现出可接受的CYP诱导电位。 还提供了分离的肽。
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