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    MULTIPLEXED EXTERNAL CALIBRATOR AND CONTROL FOR SCREENING AND DIAGNOSTIC ASSAYS
    1.
    发明申请
    MULTIPLEXED EXTERNAL CALIBRATOR AND CONTROL FOR SCREENING AND DIAGNOSTIC ASSAYS 有权

    公开(公告)号：US20220308066A1

    公开(公告)日：2022-09-29

    申请号：US17419844

    申请日：2019-10-16

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Jason Cournoyer ,  Subhakar Dey ,  Amy E. Trudel ,  Subhasish Purkayastha

    IPC: G01N33/68 ,  G01N33/74

    Abstract: Methods, compositions, kits, and apparatuses for MS-based quantification of a target analyte (e.g., a peptide, a hormone) in a sample are provided for increasing the productivity and/or throughput of samples by reducing the time and/or cost associated with conventional techniques for external instrument calibration that typically utilize a series of calibrators that are sequentially run through the same analytical process as a batch of samples. In various aspects, calibration mixtures are provided containing a known quantity of a plurality of calibrants for a target analyte of interest, with each calibrant being distinguishable in the calibration mixture by mass spectrometry such that a complete calibration curve can be generated from a single external calibration run.

    Microbial identification and quantitation using MS cleavable tags
    3.
    发明授权
    Microbial identification and quantitation using MS cleavable tags 有权

    公开(公告)号：US10281468B2

    公开(公告)日：2019-05-07

    申请号：US15118850

    申请日：2015-02-25

    Applicant: DH Technologies Development Pte. Ltd.

    Inventor： Subhasish Purkayastha ,  Subhakar Dey

    IPC: G01N33/569 ,  G01N30/88 ,  C12Q1/04 ,  C12Q1/02 ,  C12Q1/68 ,  G01N30/72 ,  C12Q1/686 ,  G01N33/68

    Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to select a peptide labeled with a first tag of a known microbe, fragment the labeled peptide of the known microbe, and monitor for an intensity of the first tag in an MRM method using a processor. An ion source provides a beam of ions from a sample that includes peptides labeled with the first tag. The first tag binds to a peptide of a known microbe and is cleaved from the peptide of the known microbe during mass spectrometry. The mass spectrometer receives the beam of ions and is adapted to perform the MRM method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample using the processor.

    ENHANCEMENT OF SENSITIVITY AND SPECIFICITY OF KETOSTEROIDS AND KETO OR ALDEHYDE CONTAINING ANALYTES
    4.
    发明申请
    ENHANCEMENT OF SENSITIVITY AND SPECIFICITY OF KETOSTEROIDS AND KETO OR ALDEHYDE CONTAINING ANALYTES 审中-公开
    提高酮类和包含分析物的酮或酮酸的灵敏度和特异性

    公开(公告)号：US20140349885A1

    公开(公告)日：2014-11-27

    申请号：US14368358

    申请日：2012-12-06

    Applicant: DH Technologies development Pte. Ltd.

    Inventor： Subhakar Dey ,  Subhasish Purkayastha

    IPC: G01N33/74 ,  G01N33/58

    CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/58 ,  G01N2030/042 ,  G01N2458/15 ,  G01N2560/00 ,  Y10T436/200833

    Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

    Abstract translation: 提供酮或醛化合物的相对定量，绝对定量或两者的方法，标记试剂，组标记试剂和标记技术，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物基质中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

    QUANTITATIVE ANALYSIS OF VITAMIN D3, VITAMIN D2, AND METABOLITES THEREOF
    5.
    发明申请
    QUANTITATIVE ANALYSIS OF VITAMIN D3, VITAMIN D2, AND METABOLITES THEREOF 审中-公开
    维生素D3，维生素D2及其代谢物的定量分析

    公开(公告)号：US20140127825A1

    公开(公告)日：2014-05-08

    申请号：US14104117

    申请日：2013-12-12

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

    IPC: G01N33/82

    CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

    Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

    Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。

    Microbial identification and quantitation using MS cleavable tags
    6.
    发明授权
    Microbial identification and quantitation using MS cleavable tags 有权

    公开(公告)号：US11549944B2

    公开(公告)日：2023-01-10

    申请号：US16358533

    申请日：2019-03-19

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： Subhasish Purkayastha ,  Subhakar Dey

    IPC: G01N33/569 ,  C12Q1/02 ,  G01N30/72 ,  C12Q1/68 ,  C12Q1/04 ,  C12Q1/686 ,  G01N30/88 ,  G01N33/68

    Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

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