公开(公告)号：US20220057412A1

公开(公告)日：2022-02-24

申请号：US17417572

申请日：2019-12-23

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar N Dey ,  Michal Weinstock

IPC: G01N33/74 ,  G01N30/04 ,  G01N30/72 ,  G01N1/34 ,  G01N1/40

Abstract: The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.

公开(公告)号：US20140004616A1

公开(公告)日：2014-01-02

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N27/62

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US20240420939A1

公开(公告)日：2024-12-19

申请号：US18704242

申请日：2022-10-26

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Michal Weinstock ,  Yongquan Lai ,  Subhasish Purkayastha ,  Scott Daniels

IPC: H01J49/04 ,  H01J49/00

Abstract: The presently claimed and described technology provides a sample processing system comprising at least one sample introduction device, wherein the at least one sample introduction device is configured to receive a sample; a mass analyzer coupled to the sample introduction device; a control system configured to at least control the at least one sample introduction device and/or the mass analyzer, wherein the mass analyzer is configured to perform a first mass analysis on the sample, wherein the first mass analysis is mass screening for an analyte of interest in the sample, and wherein if the analyte of interest is detected in the sample, the mass analyzer is configured to perform a second mass analysis, wherein the second mass analysis is a quantitative analysis, comprising: ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte; determining intensity and/or abundance of the at least one product ion transition and/or the at least one isotopic ion transition; and quantifying the at least one analyte present in the sample using the intensity and/or abundance of the at least one product ion transition and/or isotopic ion transition.

公开(公告)号：US10094809B2

公开(公告)日：2018-10-09

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N30/72 ,  G01N33/74 ,  H01J49/16 ,  H01J49/00

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US11879898B2

公开(公告)日：2024-01-23

申请号：US17417572

申请日：2019-12-23

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar N Dey ,  Michal Weinstock

IPC: G01N33/00 ,  G01N33/74 ,  G01N1/34 ,  G01N1/40 ,  G01N30/04 ,  G01N30/72 ,  G01N30/02 ,  G01N30/06

CPC classification number: G01N33/743 ,  G01N1/34 ,  G01N1/4077 ,  G01N30/04 ,  G01N30/72 ,  G01N2001/4083 ,  G01N2001/4088 ,  G01N2030/027 ,  G01N2030/045 ,  G01N2030/067 ,  G01N2560/00

Abstract: The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.

公开(公告)号：US10078091B2

公开(公告)日：2018-09-18

申请号：US14401427

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd. ,  Oregon Health and Science University

Inventor： Andrea DeBarber ,  Subhasish Purkayastha ,  Robert D. Steiner ,  Michal Weinstock

IPC: G01N33/00 ,  G01N33/92 ,  G01N33/58 ,  H01J49/00 ,  G01N33/68

CPC classification number: G01N33/92 ,  G01N33/58 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2333/90251 ,  G01N2400/00 ,  G01N2800/04 ,  G01N2800/065 ,  G01N2800/385 ,  H01J49/0031

Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the analysis of ketosterol biomarkers such as bile acid precursors from human plasma, serum or whole blood. This method is used for new born screening for Cerebrotendinous Xanthomatosis (CTX). Methods for labeling, analyzing, and quantifying ketosterol biomarkers are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US20170115258A1

公开(公告)日：2017-04-27

申请号：US15339119

申请日：2016-10-31

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillai ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N30/72 ,  G01N33/74

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US09500622B2

公开(公告)日：2016-11-22

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N27/62 ,  G01N30/72 ,  G01N33/64 ,  G01N33/74 ,  G01N30/04 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。