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31.发明授权公开(公告)号：US08173867B2
公开(公告)日：2012-05-08
申请号：US12276961
申请日：2008-11-24
申请人： Dwight T. Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
发明人： Dwight T. Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
IPC分类号： C12N15/82
CPC分类号： C12N15/827 , C07K14/415
摘要： The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.
摘要翻译： 本发明提供调节植物开花时间的组合物和方法。 公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。 还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。 该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。 这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。 提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。
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32.发明授权公开(公告)号：US07928287B2
公开(公告)日：2011-04-19
申请号：US10367417
申请日：2003-02-13
申请人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
发明人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
CPC分类号： C12N15/1082
摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
摘要翻译： 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。
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公开(公告)号：US07741534B2
公开(公告)日：2010-06-22
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20090227013A1
公开(公告)日：2009-09-10
申请号：US12463450
申请日：2009-05-11
申请人： Timothy G. Helentjaris , Xiaomu Niu
发明人： Timothy G. Helentjaris , Xiaomu Niu
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明一般涉及植物分子生物学领域。 更具体地，本发明涉及用于时间和/或空间调节基因表达的方法和试剂，特别是在植物种子和相关的雌性生殖组织中。 组合物包含称为eep2的种子优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US07091398B2
公开(公告)日：2006-08-15
申请号：US10080114
申请日：2002-02-21
CPC分类号： C12N9/1062 , C12N15/8246 , C12N15/8261 , Y02A40/146
摘要： The invention provides a novel isolated sucrose synthase nucleic acid and its encoded protein. The present invention also provides methods and compositions relating to altering sucrose synthase levels in plants, and in particular, in plant stalks and/or plant seeds. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seeds.
摘要翻译： 本发明提供了一种新的分离的蔗糖合酶核酸及其编码的蛋白质。 本发明还提供了与植物中特别是在植物茎和/或植物种子中改变蔗糖合成酶水平相关的方法和组合物。 本发明还提供了重组表达盒，宿主细胞，转基因植物和转基因种子。
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公开(公告)号：US20050198702A1
公开(公告)日：2005-09-08
申请号：US10138014
申请日：2002-05-03
申请人： Shuping Jiao , Jeffrey Habben , Xiaomu Niu
发明人： Shuping Jiao , Jeffrey Habben , Xiaomu Niu
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/8234
摘要： The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a seed-preferred promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the seed-preferred promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。 该组合物是种子优选启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为含有与本发明的种子优选启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US09205465B2
公开(公告)日：2015-12-08
申请号：US13154141
申请日：2011-06-06
申请人： Daniel M. Goldman , Xiaomu Niu
发明人： Daniel M. Goldman , Xiaomu Niu
摘要： The present invention provides a method, apparatus, and system for efficient, high throughput, consistent cleaning of soil from the root system of a plant. In one embodiment, the method generally comprises positioning a plant adjacent a sprayer assembly having at least one nozzle angled so as to impact the root system at a predetermined position, providing a stream of water from a reservoir through the nozzle of the sprayer assembly, and rotating at least one of the root system and the nozzle relative to the other to remove the soil from the root system. The present invention further describes a recycling system adapted to recycle water expended during the cleaning process to minimize losses and to capture and return dislodged soil to the field from which the plant is extracted.
摘要翻译： 本发明提供了一种用于从植物根系中有效，高通量，一致地清洁土壤的方法，装置和系统。 在一个实施例中，该方法通常包括将具有至少一个喷嘴的喷雾器组件相邻的设备定位成角度以便在预定位置冲击根系，从储存器通过喷雾器组件的喷嘴提供水流;以及 使根系和喷嘴中的至少一个相对于另一个旋转，以从根系中去除土壤。 本发明进一步描述了一种循环系统，其适于循环在清洁过程中消耗的水以使损失最小化并捕获并返回移出的土壤到植物被提取的田地。
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公开(公告)号：US08247649B2
公开(公告)日：2012-08-21
申请号：US12208592
申请日：2008-09-11
申请人： Xiaomu Niu , Bruce J Drummond
发明人： Xiaomu Niu , Bruce J Drummond
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a root-preferred promoter and terminator isolated from the maize MSY coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从玉米MSY编码区分离的根优选启动子和终止子的新型核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US07544857B2
公开(公告)日：2009-06-09
申请号：US11589656
申请日：2006-10-30
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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公开(公告)号：US06921815B2
公开(公告)日：2005-07-26
申请号：US10109488
申请日：2002-03-28
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
IPC分类号： C07K14/415 , C12N9/06 , C12N15/82 , C12N15/00 , C12N15/09
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8223 , C12N15/8295
摘要： The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。 该组合物是启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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