公开(公告)号：US20170016060A1

公开(公告)日：2017-01-19

申请号：US15125124

申请日：2015-03-11

Applicant: ILLUMINA, INC.

Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

Abstract: The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

Abstract translation: 所公开的实施例涉及用于检测生物反应的微流体盒。 在一些实施方案中，微流控盒被配置为对核酸样品进行测序操作。 在一个方面，微流控筒包括限定用于处理要测序的核酸样品的通道和阀的流体层叠层，以及集成在堆中的固态CMOS生物传感器。 生物传感器具有被配置为检测生物反应的信号的有源区域，其中在操作期间基本上所有的有效区域可用于试剂递送和照明。 在另一方面，微流控盒包括：（a）流动池，包括包含一个或多个反应位点的反应位点区域; （b）用于将反应物输送到和/或从反应部位区域除去反应物的流体通道; （c）生物传感器，其具有被配置为检测反应部位区域中的生物反应信号的有源区域。 反应部位区域靠近生物传感器的有效区域，反应部位区域基本上跨越生物传感器的所有活性区域。 在一些实施例中，流体通道基本上与生物传感器的有效区域重叠。 还公开了用于制造和操作微流控盒的方法。

公开(公告)号：US12123833B2

公开(公告)日：2024-10-22

申请号：US18512284

申请日：2023-11-17

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

CPC classification number: G01N21/6428 ,  G01N21/6454 ,  G01N33/53 ,  G01N2021/6471 ,  G01N2201/08

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US20240118188A1

公开(公告)日：2024-04-11

申请号：US18542840

申请日：2023-12-18

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/6874

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N15/1404 ,  B01L2300/0654 ,  B01L2300/168

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US20220323956A1

公开(公告)日：2022-10-13

申请号：US17848840

申请日：2022-06-24

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Tarun Khurana ,  Behnam Javanmardi ,  Poorya Sabounchi ,  Majid Aghababazadeh

IPC: B01L3/00 ,  C12Q1/6874 ,  C12Q1/6869 ,  B01L7/00

Abstract: A flow cell including inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween. The flow channel includes a diffuser region and a field region that is located downstream from the diffuser region. The field region of the flow channel directs fluid along reaction sites where desired reactions occur. The fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction. The first and second flow directions being substantially perpendicular.

公开(公告)号：US11332788B2

公开(公告)日：2022-05-17

申请号：US17153379

申请日：2021-01-20

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Yan-You Lin ,  M. Shane Bowen ,  Cyril Delattre ,  Fabien Abeille ,  Tarun Khurana ,  Arnaud Rival ,  Poorya Sabounchi ,  Dajun Yuan ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/6874 ,  C12Q1/6806 ,  G03F7/00 ,  B05D3/06 ,  G03F7/20

Abstract: Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

公开(公告)号：US20210318999A1

公开(公告)日：2021-10-14

申请号：US17304758

申请日：2021-06-25

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

公开(公告)号：US11080248B2

公开(公告)日：2021-08-03

申请号：US15995767

申请日：2018-06-01

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

公开(公告)号：US10883937B2

公开(公告)日：2021-01-05

申请号：US16778935

申请日：2020-01-31

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US10767219B2

公开(公告)日：2020-09-08

申请号：US15125124

申请日：2015-03-11

Applicant: Illumina, Inc.

Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

IPC: B01L3/00 ,  C12Q1/6869 ,  B01L7/00 ,  C12Q1/6806 ,  C40B60/06

Abstract: The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

公开(公告)号：US10551317B2

公开(公告)日：2020-02-04

申请号：US16206564

申请日：2018-11-30

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.