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47 条记录 (耗时 0.022 秒)

    Enveloped miroorganism
    34.
    发明申请
    Enveloped miroorganism 审中-公开
    包容的微生物

    公开(公告)号：US20050244374A1

    公开(公告)日：2005-11-03

    申请号：US10504944

    申请日：2003-02-13

    申请人： Werner Goebel ,  Ulf Rapp ,  Hans-Harald Sedlacek ,  Joachim Fensterle ,  Ivaylo Gentschev

    发明人： Werner Goebel ,  Ulf Rapp ,  Hans-Harald Sedlacek ,  Joachim Fensterle ,  Ivaylo Gentschev

    IPC分类号： C12N15/09 ,  A61K35/74 ,  A61K35/76 ,  A61K39/00 ,  A61K48/00 ,  A61P29/00 ,  A61P35/00 ,  A61P35/02 ,  A61P37/02 ,  A61P37/06 ,  C07K14/705 ,  C07K14/765 ,  C12N1/00 ,  C12N1/21 ,  C12N7/04 ,  C12N9/24 ,  C12N15/63 ,  C12N15/74

    CPC分类号： C12Y302/01031 ,  A61K48/00 ,  A61K48/0008 ,  C07K14/70575 ,  C07K14/765 ,  C07K2319/02 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/2434 ,  C12N15/74 ,  C12Y301/16001

    摘要： The invention relates to an enveloped microorganism in whose genome the following components are inserted and can be expressed: I) a nucleotide sequence that encodes a directly or indirectly, antiproliferatively or cytotoxically active expression product or a plurality of said expression products, II) a nucleotide sequence that encodes or is constitutively active for a blood plasma protein under the control of a activation sequence that can be activated in the microorganism, III) optionally, a nucleotide sequence that encodes or is constitutively active for a cell-specific ligand under the control of an activation sequence that can be activated in the microorganism, IV) a nucleotide sequence for a transport system that induces expression of the expression products of components I) and II) and optionally III) on the outer surface of the microorganism or that induces secretion of the expression products of component I) and expression of component II) and optionally component III) and that is preferably constitutively active, V) optionally a nucleotide sequence for a protein used for lysis of the microorganism in the cytosol of mammalian cells and for the intracellular release of plasmids with at least one or more components I) and VI) contained in the lysed microorganism, and VI) an activation sequence that can be activated in the microorganism, and/or that is tissue-specific, tumor cell-specific, function-specific or not cell-specific, for expressing component I). The inventive microorganism is further characterized in that any of components I) to VI) can be present either single or several times, and can be either identical or different.

    摘要翻译： 本发明涉及其包含的微生物，其基因组中插入以下组分并可以表达：I）编码直接或间接抗增殖或细胞活性的表达产物或多种所述表达产物的核苷酸序列，II）核苷酸 在可在微生物中活化的活化序列的控制下编码或组成型的血浆蛋白质的序列，III）任选地，编码或组成型活性的细胞特异性配体的控制下的核苷酸序列 可以在微生物中活化的活化序列，IV）诱导表达产物的转运体系的核苷酸序列，其诱导组分I）和II）和任选地III）的表达产物在微生物的外表面上，或诱导分泌 组分I）和组分II）和任选的组分III）和tha的表达产物 t优选是组成型活性的，V）任选的用于在哺乳动物细胞的细胞溶质中裂解微生物的蛋白质的核苷酸序列，以及用裂解的细胞内含有至少一种或多种组分I）和VI）的质粒的细胞内释放的核苷酸序列 微生物和VI）可以在微生物中活化的活化序列和/或组织特异性，肿瘤细胞特异性，功能特异性或非细胞特异性的表达组分I）的活化序列。 本发明的微生物的特征还在于组分I）至VI）中的任何一种可以单独或多次存在，并且可以是相同的或不同的。

    Complex-forming proteins
    35.
    发明授权
    Complex-forming proteins 失效
    复合物形成蛋白

    公开(公告)号：US06495346B1

    公开(公告)日：2002-12-17

    申请号：US09481593

    申请日：2000-01-12

    申请人： Valerie Jerome ,  Hans-Harald Sedlacek ,  Rolf Müller

    发明人： Valerie Jerome ,  Hans-Harald Sedlacek ,  Rolf Müller

    IPC分类号： C12N1562

    CPC分类号： C07K7/08 ,  A61K38/00 ,  C07K14/82 ,  C07K2319/00 ,  C07K2319/09 ,  C07K2319/50 ,  C07K2319/71 ,  C07K2319/73 ,  C07K2319/81 ,  C12N15/1055 ,  C12N15/62

    摘要： The invention relates to a complex of specifically complex-forming proteins which are not naturally occurring, comprising the following components: a) at least one ligand specific for a target structure, b) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component c) and the component b) being connected covalently to the component a), c) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component b) and the component c) is linked covalently to the component d), and d) at least one effector. In addition, the invention relates to the use and preparation of these complexes, and to nucleic acid constructs coding for the proteins mentioned and use thereof.

    摘要翻译： 本发明涉及不是天然存在的特异性复合物形成蛋白质的复合物，其包含以下组分：a）至少一种对靶结构特异的配体，b）至少一种包含突变二聚化结构域的蛋白质，所述突变的二聚化 已经通过天然存在的二聚化结构域的突变产生的结构域，该突变的二聚化结构域可能与组分c）特异性相互作用，并且组分b）与组分a）共价连接，c）至少一种蛋白质，其包含 突变的二聚化结构域，突变的二聚化结构域是通过天然存在的二聚化结构域的突变产生的，该突变的二聚化结构域可能与组分b）特异性相互作用，并且组分c）与组分d）共价连接， d）至少一个效应器。 此外，本发明涉及这些复合物的使用和制备，以及编码所述蛋白质的核酸构建体及其用途。

    Target cell-specific non-viral vectors for inserting genes into cells, pharmaceutical compositions comprising such vectors and their use
    39.
    发明授权
    Target cell-specific non-viral vectors for inserting genes into cells, pharmaceutical compositions comprising such vectors and their use 失效
    用于将基因插入细胞的靶细胞特异性非病毒载体，包含这种载体的药物组合物及其用途

    公开(公告)号：US06358524B1

    公开(公告)日：2002-03-19

    申请号：US09280068

    申请日：1999-03-29

    申请人： Hans-Harald Sedlacek ,  Hans-Dieter Klenk ,  Thomas Kissel ,  Rolf Müller

    发明人： Hans-Harald Sedlacek ,  Hans-Dieter Klenk ,  Thomas Kissel ,  Rolf Müller

    IPC分类号： A61K9127

    CPC分类号： C12N15/88 ,  A61K47/51 ,  A61K48/00 ,  C12N15/87

    摘要： Target cell-specific, non-viral vectors for inserting genes into cells, pharmaceuticals composition comprising such vectors, and methods of their use. Target cell-specific, non-viral vectors for inserting at least one gene into cells of an organism, comprising a complex comprising the following components: a) a non-viral carrier for the gene to be inserted, b) a ligand which can bind specifically to the desired target cell, c) a fusion protein for the penetration of the vector into the cytoplasm of the target cell, and d) the gene to be introduced are disclosed. Vectors of this nature are used, for example, in gene therapy.

    摘要翻译： 用于将基因插入细胞的靶细胞特异性非病毒载体，包含这种载体的药物组合物及其使用方法。 用于将至少一个基因插入生物体细胞的靶细胞特异性非病毒载体，其包含包含以下组分的复合物：a）待插入基因的非病毒载体，b）可结合的配体 具体涉及所需的靶细胞，c）用于将载体渗透入靶细胞的细胞质的融合蛋白，以及d）将要引入的基因。 这种性质的载体用于例如基因治疗。