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    Process for producing recombinant proteins using a gene for tRNA
    31.
    发明授权
    Process for producing recombinant proteins using a gene for tRNA 失效
    使用tRNA基因生产重组蛋白的方法

    公开(公告)号：US06270988B1

    公开(公告)日：2001-08-07

    申请号：US08009423

    申请日：1993-01-27

    申请人： Ulrich Brinkmann ,  Ralf Mattes ,  Stephan Fischer

    发明人： Ulrich Brinkmann ,  Ralf Mattes ,  Stephan Fischer

    IPC分类号： C12P2102

    CPC分类号： C12N9/6456 ,  C12N15/11 ,  C12N15/67 ,  C12N15/70

    摘要： The present invention provides a process for the expression of a recombinant gene, which contains AGA and/or AGG codons for arginine, in Escherichia coli after transformation with an expression vector which contains the recombinant gene, wherein the amount of t-RNA present in the E. coli cells which incorporates arginine and recognises the codons AGG and AGA is increased to at least fivefold of the amount normally occurring in these cells.

    摘要翻译： 本发明提供了在含有重组基因的表达载体转化后，在大肠杆菌中表达含有精氨酸的AGA和/或AGG密码子的重组基因的方法，其中存在于 将精氨酸并且识别密码子AGG和AGA的大肠杆菌细胞增加至通常在这些细胞中发生的量的至少5倍。

    Mutant of the Erythrina caffra type inhibitor and the use of the said mutant for purifying serine proteases
    32.
    发明授权
    Mutant of the Erythrina caffra type inhibitor and the use of the said mutant for purifying serine proteases 失效
    Erythrina caffra型抑制剂的突变体和所述突变体用于纯化丝氨酸蛋白酶的用途

    公开(公告)号：US5973118A

    公开(公告)日：1999-10-26

    申请号：US943814

    申请日：1997-10-03

    申请人： Ulrich Kohnert ,  Anne Stern ,  Manfred Wozny ,  Stephan Fischer

    发明人： Ulrich Kohnert ,  Anne Stern ,  Manfred Wozny ,  Stephan Fischer

    IPC分类号： C12N15/09 ,  C07K1/22 ,  C07K14/81 ,  C12N1/21 ,  C12N9/64 ,  C12N9/72 ,  C12N9/99 ,  C12N15/29 ,  C12P21/02 ,  C12R1/19 ,  C07K1/00 ,  A61K35/78 ,  A61K38/00

    CPC分类号： C12N9/6459 ,  C07K14/8114 ,  C12Y304/21069

    摘要： A polypeptide which has the activity of an inhibitor DE-3 from Erythrina caffra and which reversibly and selectively binds serine proteases from a protein mixture is obtainable by culturing prokaryotic or eukaryotic host cells which have been transformed or transfected with a nucleic acid that codes for the said polypeptide in a manner that allows the host cells to express the said polypeptide under suitable nutrient conditions and isolating the said polypeptide, wherein the polypeptide has an amino acid sequence which is functionally analogous to SEQ ID NO:2, has a partial region that is more than 85% homologous to the region of amino acids 39-139 of this sequence, has two disulfide bridges and begins N-terminally with SEQ ID NO:4 or with a SEQ ID NO:4 extended N-terminally by methionine and has a binding capacity for tissue plasminogen activators of 1.25 MU/ml and more and is particularly suitable for purifying plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).

    摘要翻译： 通过培养原核或真核宿主细胞获得具有来自艾瑞氏菌（Erythrina caffra）的抑制剂DE-3并且可逆地和选择性地结合蛋白质混合物的丝氨酸蛋白酶的多肽，所述原核或真核宿主细胞已经用编码 所述多肽以允许宿主细胞在合适的营养条件下表达所述多肽并分离所述多肽的方式，其中所述多肽具有功能上类似于SEQ ID NO：2的氨基酸序列，其部分区域是 与该序列的氨基酸39-139的区域同源超过85％具有两个二硫键，并与SEQ ID NO：4进行N-末端或与蛋氨酸N-末端扩增的SEQ ID NO：4，并具有 组织纤溶酶原激活剂的结合能力为1.25 MU / ml以上，特别适用于纯化纤溶酶原激活剂如组织纤溶酶原激活剂（t-PA和de rivatives）。

    Thrombolytically active non-glycosylated protein
    33.
    发明授权
    Thrombolytically active non-glycosylated protein 失效
    紫外线活性非糖蛋白

    公开(公告)号：US5223256A

    公开(公告)日：1993-06-29

    申请号：US585129

    申请日：1990-09-28

    申请人： Anne Stern ,  Ulrich Kohnert ,  Rainer Rudolph ,  Stephan Fischer ,  Ulrich Martin

    发明人： Anne Stern ,  Ulrich Kohnert ,  Rainer Rudolph ,  Stephan Fischer ,  Ulrich Martin

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61K38/48 ,  A61K38/49 ,  A61P7/02 ,  C07K1/113 ,  C07K14/745 ,  C12N9/64 ,  C12N9/72 ,  C12N9/88 ,  C12N15/58

    CPC分类号： C12N9/6459 ,  C07K1/1133 ,  C12Y304/21069 ,  A61K38/00

    摘要： A new thrombolytically active protein is not glycosylated and consists of the following amino acid sequence: ##STR1## or said amino acid sequence with an additional N-terminal methionine. DNA sequences encoding the thrombolytically active, non-glycosylated protein and pharmaceutical compositions containing said protein are also disclosed. The protein possesses particularly favorable properties when used to dissolve blood clots.

    摘要翻译： PCT No.PCT / EP90 / 00194 Sec。 371 1990年9月28日第 102（e）1990年9月28日PCT PCT 1990年2月6日PCT公布。 第WO90 / 09437号公报 日期8月23日，1990.A新血栓溶解活性蛋白未被糖基化，它包括以下氨基酸序列组成：1SYQGNSDCYFGNGSAYRGTHSLTESGASCL PWNSMILIGKVYTAQNPSAQ 51ALGLGKHNYCRNPDGDAKPWCHVLKNRRLT WEYCDVPSCSTCGLRQYSQP 101QFRIKGGLFADIASHPWQAAIFAKHRRSPG ERFLCGGILISSCWILSAAH 151CFQERFPPHHLTVILGRTYRVVPGEEEQKF EVEKYIVHKEFDDDTYDNDI 201ALLQLKSDSSRCAQESSVVRTVCLPPADLQ LPDWTECELSGYGKHEALSP 251FYSERLKEAHVRLYPSSRCTSQHLLNRTVT DNMLCAGDTRSGGPQANLHD 301ACQGDSGGPLVCLNDGRMTLVGIISWGLGC GQKDVPGVYTKVTNYLDWIR 351DNMRP或所述具有附加的N末端氨基酸序列 甲硫氨酸 还公开了编码血栓溶解活性的非糖基化蛋白质的DNA序列和含有所述蛋白质的药物组合物。 当用于溶解血块时，蛋白质具有特别有利的特性。

    Device for cooling a metal strip
    34.
    发明授权
    Device for cooling a metal strip 有权
    用于冷却金属条的装置

    公开(公告)号：US08434338B2

    公开(公告)日：2013-05-07

    申请号：US12449138

    申请日：2007-12-17

    申请人： Jürgen Armenat ,  Stephan Fischer

    发明人： Jürgen Armenat ,  Stephan Fischer

    IPC分类号： B21B27/06

    CPC分类号： B21B45/0218

    摘要： The invention relates to a device (1) for cooling a metal strip (2) between two rolling stands (3), wherein the strip (2) is led over a leading-over element (4) of a flat form and arranged under the leading-over element (4) is a spraying element (5), which directs cooling medium (7) onto the underside (8) of the strip (2) through at least one opening (6) in the leading-over element (4). To achieve an improved spray pattern, the invention provides that at least two openings (6) are introduced into the leading-over element (4), said openings being arranged next to each other in a direction transverse (Q) to the conveying direction (F) of the strip (2) and having an elongated form, wherein the longitudinal axis (9) of the opening (6) is aligned at an angle (α) with respect to the conveying direction (F) of the strip.

    摘要翻译： 本发明涉及一种用于冷却两个轧机机座（3）之间的金属带（2）的装置（1），其中，带（2）被引导在平面形式的引导元件（4）上， 引导元件（4）是喷射元件（5），其通过引导元件（4）中的至少一个开口（6）将冷却介质（7）引导到条带（2）的下侧（8） ）。 为了实现改进的喷雾图案，本发明提供了至少两个开口（6）被引入到引导元件（4）中，所述开口沿着与输送方向横向（Q）的方向彼此相邻地布置 （2）的F）并且具有细长形式，其中开口（6）的纵向轴线（9）相对于条带的输送方向（F）成一角度（α）对准。

    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL)
    35.
    发明申请
    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 审中-公开
    产生胰岛素样生长因子-1和聚（乙二醇）的方法

    公开(公告)号：US20100035817A1

    公开(公告)日：2010-02-11

    申请号：US12576266

    申请日：2009-10-09

    申请人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    发明人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    IPC分类号： A61K38/18 ,  A61P25/28

    CPC分类号： C07K14/65 ,  A61K47/60 ,  C07K2319/21 ,  C07K2319/50

    摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.

    摘要翻译： 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。

    Dispenser for razor cartridges
    37.
    发明授权
    Dispenser for razor cartridges 有权
    剃须刀盒分配器

    公开(公告)号：US07413078B2

    公开(公告)日：2008-08-19

    申请号：US11396802

    申请日：2006-04-03

    申请人： Stephan Fischer ,  Jochen Thoene

    发明人： Stephan Fischer ,  Jochen Thoene

    IPC分类号： A45C11/26

    CPC分类号： A45D27/225 ,  B26B21/40

    摘要： According to one aspect of the present invention, a dispenser for one or more razor cartridges includes a base, two side walls, a front wall, a rear wall, a first divider, a second divider, a cover, and a side spring. The first divider is located between the front wall and the rear wall. The second divider is located between the first divider and the front wall, forming a chamber between the second divider and the first divider. The chamber is sized and shaped to receive at least a portion of a razor cartridge. The cover extends partially over the chamber between the first divider and second divider, and is sized to provide an opening through which the razor cartridge can be inserted into the chamber. The side spring extends widthwise into the chamber. The cover and the side spring frictionally engage the razor cartridge when inserted into the chamber.

    摘要翻译： 根据本发明的一个方面，用于一个或多个剃刀刀片架的分配器包括底座，两个侧壁，前壁，后壁，第一分隔件，第二分隔件，盖和侧弹簧。 第一个分隔器位于前壁和后壁之间。 第二分隔件位于第一分隔件和前壁之间，在第二分隔件和第一分隔件之间形成一个室。 该腔室的尺寸和形状被设计成容纳至少一部分剃须刀片架。 盖子部分地延伸在第一分隔件和第二分隔件之间的腔室之上，并且其尺寸被设计成提供开口，通过该开口可以将剃刀刀片架插入腔室。 侧面弹簧沿宽度方向延伸进入腔室。 当插入腔室时，盖子和侧面弹簧摩擦地接合剃刀刀片架。