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23 条记录 (耗时 0.019 秒)

    Process for the highly specific detection of nucleic acids in solid
    7.
    发明授权
    Process for the highly specific detection of nucleic acids in solid 失效
    在溶液中高度特异性检测核酸的方法

    公开(公告)号：US5354657A

    公开(公告)日：1994-10-11

    申请号：US993820

    申请日：1992-12-18

    申请人： Hans J. Holtke ,  Rudolf Seibl ,  Christoph Kessler ,  Ralf Mattes ,  Hermann Graf

    发明人： Hans J. Holtke ,  Rudolf Seibl ,  Christoph Kessler ,  Ralf Mattes ,  Hermann Graf

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C07H21/00 ,  C12Q1/6804

    摘要： The present invention provides a process for the detection of nucleic acids of definite sequence by hybridisation with two single-stranded nucleic acid probes present in the same solution phase complementary to different regions of the nucleic acid to be detected, one nucleic acid probe serving as detector probe and containing as labelling at least one hapten bound via a chemical linkage and the other nucleic acid probe serving as capturing probe and being bound to a solid matrix, wherein, as hapten, a steroid is used which on at least one position of the detector probe, which does not participate in the hydrogen bridge formation with the nucleic acid to be detected, is covalently bound via a bridge of at least four atoms, the nucleic acid to be detected, present in solution, is incubated with the detector probe and the capturing probe in any desired sequence, whereby the binding of the capturing probe on the matrix with the nucleic acid to be detected is brought about before, during or after the incubation, the remaining solution is separated off from the matrix-bound complex of the capturing probe, nucleic acid to be detected and detector probe and the complex detected via an anti-hapten antibody which is labelled.

    摘要翻译： 本发明提供了通过与存在于​​与要检测的核酸的不同区域互补的相同溶液相中的两个单链核酸探针杂交来检测确定序列的核酸的方法，一个用作检测器的核酸探针 探针并包含作为标记至少一个通过化学连接结合的半抗原，并且另一个核酸探针用作捕获探针并结合到固体基质上，其中作为半抗原，使用类固醇，其在检测器的至少一个位置上 不参与与要检测的核酸的氢桥形成的探针通过至少四个原子的桥共价结合，将存在于溶液中的要检测的核酸与检测器探针和 以任何期望的顺序捕获探针，由此捕获探针在基质上与待检测的核酸的结合在 r后，将剩余的溶液与捕获探针的基质结合复合物，待检测的核酸和检测器探针分离，并通过经标记的抗半抗原抗体检测复合物。

    Process for producing recombinant proteins using a gene for tRNA
    10.
    发明授权
    Process for producing recombinant proteins using a gene for tRNA 失效
    使用tRNA基因生产重组蛋白的方法

    公开(公告)号：US06270988B1

    公开(公告)日：2001-08-07

    申请号：US08009423

    申请日：1993-01-27

    申请人： Ulrich Brinkmann ,  Ralf Mattes ,  Stephan Fischer

    发明人： Ulrich Brinkmann ,  Ralf Mattes ,  Stephan Fischer

    IPC分类号： C12P2102

    CPC分类号： C12N9/6456 ,  C12N15/11 ,  C12N15/67 ,  C12N15/70

    摘要： The present invention provides a process for the expression of a recombinant gene, which contains AGA and/or AGG codons for arginine, in Escherichia coli after transformation with an expression vector which contains the recombinant gene, wherein the amount of t-RNA present in the E. coli cells which incorporates arginine and recognises the codons AGG and AGA is increased to at least fivefold of the amount normally occurring in these cells.

    摘要翻译： 本发明提供了在含有重组基因的表达载体转化后，在大肠杆菌中表达含有精氨酸的AGA和/或AGG密码子的重组基因的方法，其中存在于 将精氨酸并且识别密码子AGG和AGA的大肠杆菌细胞增加至通常在这些细胞中发生的量的至少5倍。