公开(公告)号：US10066235B2

公开(公告)日：2018-09-04

申请号：US15258901

申请日：2016-09-07

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/84 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

公开(公告)号：US10006035B2

公开(公告)日：2018-06-26

申请号：US15151383

申请日：2016-05-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/84 ,  C12R1/41 ,  C12N15/82 ,  G01N33/50

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12R1/41 ,  G01N33/5097

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

公开(公告)号：US20170342426A1

公开(公告)日：2017-11-30

申请号：US15670668

申请日：2017-08-07

Applicant: Monsanto Technology LLC

Inventor： Toni Armstrong ,  Kenneth Barton ,  Laura J. Crow ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian J. Martinell ,  Michael W. Petersen ,  Kenneth J. Piller ,  Qi Wang ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

公开(公告)号：US20170009246A1

公开(公告)日：2017-01-12

申请号：US15258901

申请日：2016-09-07

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Abstract translation: 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US20150232865A1

公开(公告)日：2015-08-20

申请号：US14642674

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/82

CPC classification number: C12N15/8205

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

Abstract translation: 提供了用于改善细菌介导的植物转化的方法和组合物。 这些方法通常允许具有减少的载体骨架整合和高频率的低拷贝转化事件的植物转化。 描述了用于实现这些结果的载体，以及它们使用的方法。

公开(公告)号：US20150052642A1

公开(公告)日：2015-02-19

申请号：US14455747

申请日：2014-08-08

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Michael W. Petersen ,  Larry A. Gilbertson ,  David Walters ,  Susan J. Johnson ,  Shihshieh Huang ,  Paul S. Chomet

IPC: C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/6895 ,  A01H1/04 ,  C12N9/1085 ,  C12N15/8205 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

Abstract translation: 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

公开(公告)号：US11713465B2

公开(公告)日：2023-08-01

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/00 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

公开(公告)号：US20230057290A1

公开(公告)日：2023-02-23

申请号：US17818913

申请日：2022-08-10

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Anna Mary O'Keefe ,  David Alan Somers ,  Edward James Williams ,  Xudong Ye

IPC: C12N15/82

Abstract: Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and/or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.

公开(公告)号：US20220340916A1

公开(公告)日：2022-10-27

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/82

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

公开(公告)号：US20210163977A1

公开(公告)日：2021-06-03

申请号：US17079348

申请日：2020-10-23

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Shishieh Huang ,  Susan J. Johnson ,  Michael W. Petersen ,  David Walters ,  Paul S. Chomet

IPC: C12N15/82 ,  C12N9/10

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.