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公开(公告)号：US11597940B2
公开(公告)日：2023-03-07
申请号：US17026618
申请日：2020-09-21
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
IPC: C12N15/82
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
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公开(公告)号：US20210087580A1
公开(公告)日：2021-03-25
申请号：US16988589
申请日：2020-08-07
Applicant: Monsanto Technology LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US20210010011A1
公开(公告)日：2021-01-14
申请号：US16931315
申请日：2020-07-16
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Michael W. Petersen
Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
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4.发明申请公开(公告)号：US20190002904A1
公开(公告)日：2019-01-03
申请号：US16035556
申请日：2018-07-13
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Larry A. Gilbertson , Xudong Ye
IPC: C12N15/82
Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
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公开(公告)号：US09540700B2
公开(公告)日：2017-01-10
申请号：US14455747
申请日：2014-08-08
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Michael W. Petersen , Larry A. Gilbertson , David Walters , Susan J. Johnson , Shihshieh Huang , Paul S. Chomet
CPC classification number: C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
Abstract translation: 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。
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Patent Agency Ranking
公开(公告)号：US20160237453A1
公开(公告)日：2016-08-18
申请号：US15130684
申请日：2016-04-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
CPC classification number: C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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7.发明申请Methods For Enhancing Segregation of Transgenes in Plants and Compositions Thereof 审中-公开用于增强植物及其组合物中转基因分离的方法公开(公告)号：US20160024512A1
公开(公告)日：2016-01-28
申请号：US14862734
申请日：2015-09-23
Applicant: Monsanto Technology LLC
Inventor： Toni Armstrong , Kenneth Barton , Laura J. Crow , Larry A. Gilbertson , Shihshieh Huang , Yong Huang , Brian J. Martinell , Michael W. Petersen , Kenneth J. Piller , Qi Wang , Xudong Ye
IPC: C12N15/82
CPC classification number: C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
Abstract translation: 提供的组合物和方法增强了在不同物理和遗传基因座处整合到植物基因组中的具有两个T-DNA分子的转基因植物的选择。 组合物是包含含有感兴趣的基因，阳性选择标记基因和条件致死基因的T-DNA分子的新配置的DNA构建体。 本文公开的方法包括将植物细胞转化为包含本发明的DNA构建体，将植物细胞再生成植物并鉴定独立的转基因基因座，其中可选择标记基因或转基因元件可以在后代中分离。
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公开(公告)号：US20130074213A1
公开(公告)日：2013-03-21
申请号：US13677607
申请日：2012-11-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Edwards Allen , Larry A. Gilbertson , James K. Roberts , Nancy M. Houmard , Shihshieh Huang , Sergey Ivashuta
CPC classification number: C12N15/8218 , A01H1/02 , A01H5/00 , A01H5/10 , C12N15/8287 , C12N15/8289 , C12N15/829
Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
Abstract translation: 本发明提供了生产非天然杂种种子的方法。 还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。
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公开(公告)号：US12077770B2
公开(公告)日：2024-09-03
申请号：US17689613
申请日：2022-03-08
Applicant: Monsanto Technology LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. Larosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
CPC classification number: C12N15/8286 , A01N63/50 , A01N63/60 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/146
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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公开(公告)号：US11685930B2
公开(公告)日：2023-06-27
申请号：US16988589
申请日：2020-08-07
Applicant: Monsanto Technology LLC
Inventor： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
CPC classification number: C12N15/8286 , A01N63/50 , A01N63/60 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/146 , A01N63/50 , A01N63/23
Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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