公开(公告)号：US10337041B2

公开(公告)日：2019-07-02

申请号：US15116006

申请日：2015-02-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02 ,  C12N9/44 ,  C12N9/24 ,  A23L29/30 ,  C13K1/06 ,  C12N9/26

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US20170349887A1

公开(公告)日：2017-12-07

申请号：US15679805

申请日：2017-08-17

Applicant: NOVOZYMES A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12C7/04 ,  C12G3/02 ,  C12P19/14 ,  C12C5/00 ,  C12N9/26 ,  C12P7/06 ,  C12P21/02

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

公开(公告)号：US20170159090A1

公开(公告)日：2017-06-08

申请号：US15116006

申请日：2015-02-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/44 ,  C12N9/34 ,  C12N9/26

CPC classification number: C12P19/14 ,  A23L29/35 ,  C12N9/2402 ,  C12N9/2411 ,  C12N9/2428 ,  C12N9/2457 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01041 ,  C13K1/06

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US20150337277A1

公开(公告)日：2015-11-26

申请号：US14653154

申请日：2013-12-13

Applicant: Novozymes A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12G3/02 ,  C12P7/06

CPC classification number: C12N9/242 ,  C12C5/004 ,  C12C7/04 ,  C12G3/02 ,  C12N9/2414 ,  C12P7/06 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

Abstract translation: 本公开涉及具有α-淀粉酶活性的分离的多肽，编码多肽的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生多肽的方法，以及使用多肽的方法，包括在乙醇生产过程 。

公开(公告)号：US11667938B2

公开(公告)日：2023-06-06

申请号：US17465098

申请日：2021-09-02

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

CPC classification number: C12P19/14 ,  A23L29/35 ,  C12N9/2402 ,  C12N9/2411 ,  C12N9/2428 ,  C12N9/2457 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01041 ,  C13K1/06

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US20210395791A1

公开(公告)日：2021-12-23

申请号：US17465098

申请日：2021-09-02

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US11136607B2

公开(公告)日：2021-10-05

申请号：US16403919

申请日：2019-05-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US10316308B2

公开(公告)日：2019-06-11

申请号：US15842247

申请日：2017-12-14

Applicant: NOVOZYMES A/S

Inventor： Jesper Vind ,  Esben Peter Friis ,  Thomas Agsersten Poulsen ,  Michael Skjøt ,  Peter Kamp Hansen ,  Frank Winther Rasmussen ,  Steen Krogsgaard ,  Joyce Craig ,  Guillermo Coward-Kelly ,  Keiichi Ayabe

IPC: C12N9/24 ,  C12N15/00 ,  C12N9/34 ,  C12N9/30 ,  C12N15/52 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24

Abstract: The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.

公开(公告)号：US20180030428A1

公开(公告)日：2018-02-01

申请号：US15714292

申请日：2017-09-25

Applicant: Novozymes A/S

Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

IPC: C12N9/82 ,  A23L5/20

CPC classification number: C12N9/82 ,  A23L5/25 ,  C12Y305/01001

Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

公开(公告)号：US09765317B2

公开(公告)日：2017-09-19

申请号：US14653154

申请日：2013-12-13

Applicant: Novozymes A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/26 ,  C12N9/30 ,  C12G3/02 ,  C12P19/14 ,  C12P21/02 ,  C12P7/06 ,  C12C7/04 ,  C12C5/00

CPC classification number: C12N9/242 ,  C12C5/004 ,  C12C7/04 ,  C12G3/02 ,  C12N9/2414 ,  C12P7/06 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.