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公开(公告)号：US09593345B2
公开(公告)日：2017-03-14
申请号：US14435699
申请日：2013-10-14
发明人： Takashi Yamamoto , Michi Izumi Willcoxon , Lu Liu , Ute Schellenberger , Eric Schepers , Yi Zheng
IPC分类号： A01H5/00 , C12N15/32 , C12N15/82 , C07K14/325 , A01N37/18 , A01N63/02 , C07K14/245
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/245 , C07K14/325 , C07K2319/55
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests including, but not limited to, insect pests belonging to the orders Coleoptera and Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, polynucleotide constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供了从编号多肽的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其对昆虫害虫具有杀虫活性，包括但不限于属于鞘翅目和鳞翅目鳞翅目的昆虫害虫。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，多核苷酸构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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公开(公告)号：US20140007292A1
公开(公告)日：2014-01-02
申请号：US13792861
申请日：2013-03-11
发明人： David C. Cerf , James J. English , Carol A. Hendrick , Lu Liu , Jarred K. Oral , Phillip A. Patten , Barbara A. Rosen , Ute Schellenberger , Ingrid A. Udranszky , Jun-Zhi Wei , Genhai Zhu
CPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/1214 , C07K2319/24 , G01N33/68
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译： 提供了控制害虫的组成和方法。 该方法涉及用编码杀虫蛋白的核酸序列转化生物体。 特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫的核酸和细菌物种的蛋白质。 这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为用于分离其他同源（或部分同源的）基因的探针。 杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫种群，并用于生产具有杀虫活性的组合物。
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公开(公告)号：US12031142B2
公开(公告)日：2024-07-09
申请号：US16952193
申请日：2020-11-19
发明人： Jennifer Kara Barry , Ryan Michael Gerber , Lu Liu , Amy Lum , Eric Schepers , Nasser Yalpani , Genhai Zhu
CPC分类号： C12N15/8286 , A01N37/18 , A01N37/46 , A01N63/10 , A01N63/50 , A01N65/04 , C07K14/415 , Y02A40/146
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US11325949B2
公开(公告)日：2022-05-10
申请号：US16841168
申请日：2020-04-06
发明人： Scott Diehn , James J English , Lu Liu , Azalea S Ong , Jarred Kenneth Oral , Barbara Rosen , Ute Schellenberger , Ingrid Udranszky , Jun-Zhi Wei , Weiping Xie , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US11174295B2
公开(公告)日：2021-11-16
申请号：US16461495
申请日：2017-11-29
发明人： Steven D Gruver , Lu Liu , Barbara Rosen , Ute Schellenberger , Jun-Zhi Wei , Weiping Xie , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US11162112B2
公开(公告)日：2021-11-02
申请号：US16809931
申请日：2020-03-05
发明人： Jennifer Kara Barry , Ryan Michael Gerber , Lu Liu , Amy Lum , Eric Schepers , Nasser Yalpani , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US10294490B2
公开(公告)日：2019-05-21
申请号：US15274758
申请日：2016-09-23
发明人： Daniel J Altier , Jennifer K Barry , Carol A Hendrick , Lu Liu , Phillip A Patten , Claudia D Perez-Ortega , Eric J Schepers , Weiping Xie , Nasser Yalpani , Jianzhou Zhao , Xiaohong Zhong , Genhai Zhu
IPC分类号： C12N15/82 , C07K14/195 , C07K14/225 , A01N63/02 , A01N37/46
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US10006045B2
公开(公告)日：2018-06-26
申请号：US14912356
申请日：2014-08-14
发明人： Lu Liu , Jessica O'Rear , Young Jun Park , Barbara Rosen , Ute Schellenberger , Eric Schepers , Jun-Zhi Wei , Weiping Xie , Nasser Yalpani , Xiaohong Zhong , Genhai Zhu
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/21 , C12R1/40 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US09909110B2
公开(公告)日：2018-03-06
申请号：US15289993
申请日：2016-10-11
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
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公开(公告)号：US09688730B2
公开(公告)日：2017-06-27
申请号：US13792861
申请日：2013-03-11
发明人： David C. Cerf , James J. English , Carol A. Hendrick , Lu Liu , Jarred K. Oral , Phillip A. Patten , Barbara A. Rosen , Ute Schellenberger , Ingrid A. Udranszky , Jun-Zhi Wei , Genhai Zhu
CPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/1214 , C07K2319/24 , G01N33/68
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
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