公开(公告)号：US20090258436A1

公开(公告)日：2009-10-15

申请号：US12309310

申请日：2007-07-13

申请人： Peter Hornbeck ,  Valerie Goss ,  Ting-Lei Gu ,  Albrecht Moritz ,  Kimberly Lee

发明人： Peter Hornbeck ,  Valerie Goss ,  Ting-Lei Gu ,  Albrecht Moritz ,  Kimberly Lee

IPC分类号： G01N33/566 ,  C07K16/18

CPC分类号： C12Q1/485 ,  C07K16/18 ,  C07K16/40 ,  C07K16/44 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6845 ,  G01N33/6848 ,  G01N33/6872

摘要： The invention discloses novel phosphorylation sites identified in signal transduction proteins and pathways, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: adaptor/scaffold proteins, adhesion/extracellular matrix protein, apoptosis proteins, calcium binding proteins, cell cycle regulation proteins, chaperone proteins, chromatin, DNA binding/repair/replication proteins, cytoskeletal proteins, endoplasmic reticulum or golgi proteins, enzyme proteins, G/regulator proteins, inhibitor proteins, motor/contractile proteins, phosphatase, protease, Ser/Thr protein kinases, Protein kinase (Tyr)s, receptor/channel/cell surface proteins, RNA binding proteins, transcriptional regulators, tumor suppressor proteins, ubiquitan conjugating system proteins and proteins of unknown function.

摘要翻译： 本发明公开了在信号转导蛋白和途径中鉴定的新型磷酸化位点，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质，以及使用 用于此目的的试剂。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：衔接子/支架蛋白，粘附/细胞外基质蛋白，凋亡蛋白，钙结合蛋白，细胞周期调节蛋白，伴侣蛋白，染色质，DNA结合/修复/复制蛋白， 细胞骨架蛋白，内质网或高尔基体蛋白，酶蛋白，G /调节蛋白，抑制蛋白，运动/收缩蛋白，磷酸酶，蛋白酶，Ser / Thr蛋白激酶，蛋白激酶（Tyr），受体/通道/细胞表面蛋白， RNA结合蛋白，转录调节物，肿瘤抑制蛋白，泛素偶联系统蛋白和功能未知的蛋白。

公开(公告)号：US20090142777A1

公开(公告)日：2009-06-04

申请号：US11973019

申请日：2007-10-05

申请人： Valerie Goss ,  Albrecht Moritz ,  Ting-Lei Gu ,  Kimberly Lee ,  Roberto Polakiewicz

发明人： Valerie Goss ,  Albrecht Moritz ,  Ting-Lei Gu ,  Kimberly Lee ,  Roberto Polakiewicz

IPC分类号： G01N33/573 ,  G01N33/566 ,  C07K16/18 ,  C07K14/00 ,  C12N5/06

CPC分类号： C07K16/3061 ,  C07K16/40

摘要： The invention discloses 424 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Leukemia, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor/Scaffold proteins, Cytoskeletal proteins, Cellular Metabolism enzymes, G Protein/GTPase Activating/Guanine Nucleotide Exchange Factor proteins, Immunoglobulin Superfamily proteins, Inhibitor proteins, Lipid Kinases, Nuclear DNA Repair/RNA Binding/Transcription proteins, Serine/Threonine Protein Kinases, Tyrosine Kinases, Protein Phosphatases, and Translation/Transporter proteins.

摘要翻译： 本发明公开了在信号转导蛋白中鉴定的424个新的磷酸化位点和人类白血病的途径，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质 作为使用试剂用于此目的的方法。 所鉴定的磷酸化位点是以下蛋白质类型的位点：适配器/支架蛋白，细胞骨架蛋白，细胞代谢酶，G蛋白/ GTP酶激活/鸟嘌呤核苷酸交换因子蛋白，免疫球蛋白超家族蛋白，抑制蛋白，脂质激酶，核DNA 修复/ RNA结合/转录蛋白，丝氨酸/苏氨酸蛋白激酶，酪氨酸激酶，蛋白磷酸酶和翻译/转运蛋白。

公开(公告)号：US20080038752A1

公开(公告)日：2008-02-14

申请号：US11503335

申请日：2006-08-11

申请人： Albrecht Moritz ,  Kimberly Lee ,  John Rush ,  Roberto Polakiewicz

发明人： Albrecht Moritz ,  Kimberly Lee ,  John Rush ,  Roberto Polakiewicz

IPC分类号： G01N33/542 ,  G01N33/53

CPC分类号： G01N33/5041 ,  C07K16/18 ,  C07K16/3015 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/34 ,  G01N2333/91215

摘要： The invention discloses 102 novel phosphorylation sites identified in signal transduction proteins and pathways downstream of c-Src kinase, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor/Scaffold proteins, Actin Binding proteins, Cytoskeletal proteins, G protein/GTPase Activating protein/Guanine Nucleotide Exchange Factor proteins, Helicases, RNA Binding proteins, Transcription/Translation Factor or Initiation Complex proteins, Cellular Metabolism Enzymes, and Vesicle proteins.

摘要翻译： 本发明公开了在c-Src激酶下游的信号转导蛋白和途径中鉴定的102个新的磷酸化位点，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质 ，以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是以下蛋白质类型的位点：适配器/支架蛋白，肌动蛋白结合蛋白，细胞骨架蛋白，G蛋白/ GTPase激活蛋白/鸟嘌呤核苷酸交换因子蛋白，Helicases，RNA结合蛋白，转录因子/翻译因子或 起始复合蛋白​​，细胞代谢酶和囊泡蛋白。

公开(公告)号：US20070072235A1

公开(公告)日：2007-03-29

申请号：US11503096

申请日：2006-08-11

申请人： Albrecht Moritz ,  Kimberly Lee ,  John Rush ,  Roberto Polakiewicz

发明人： Albrecht Moritz ,  Kimberly Lee ,  John Rush ,  Roberto Polakiewicz

IPC分类号： G01N33/53

CPC分类号： G01N33/6842 ,  C07K16/44

摘要： The invention discloses 211 novel phosphorylation sites identified in signal transduction proteins and pathways underlying Anaplastic Large Cell Lymphoma (ALCL) involving the ALK-NPM translocation/fusion, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein Kinases (including Receptor Tyrosine Kinases), Adaptor/Scaffold Proteins, Cellular Metabolism or Miscellaneous Enzymes, Oxidoreductases, Transcription Factors, Cytoskeletal Proteins, Translation Initiation Complexes, RNA Binding Proteins, Proteases, Acetyltransferases, G protein regulators/GTPases, Helicases, Apoptosis/Cell Cycle Regulation proteins, and Hydrolases.

摘要翻译： 本发明公开了在信号转导蛋白中鉴定的211个新的磷酸化位点和涉及ALK-NPM易位/融合的间变性大细胞淋巴瘤（ALCL）的途径，并且为磷酸化位点特异性抗体和重同位素标记的肽（AQUA肽）提供了 这些磷酸化位点/蛋白质的选择性检测和定量，以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是以下蛋白质类型发生的位点：蛋白激酶（包括受体酪氨酸激酶），适配器/支架蛋白，细胞代谢或其他酶，氧化还原酶，转录因子，细胞骨架蛋白，翻译起始复合物，RNA结合蛋白， 蛋白酶，乙酰转移酶，G蛋白调节剂/ GTP酶，Helicases，细胞凋亡/细胞周期调控蛋白和水解酶。

公开(公告)号：US11877947B2

公开(公告)日：2024-01-23

申请号：US17216824

申请日：2021-03-30

申请人： Kimberly Lee

发明人： Kimberly Lee

IPC分类号： A61F5/451 ,  A61F5/44 ,  A61M1/00 ,  A61F5/453

CPC分类号： A61F5/451 ,  A61F5/4405 ,  A61F5/453 ,  A61M1/69 ,  A61M1/84 ,  A61M2202/0496

摘要： An external catheter system is provided. The system includes an elongated sheath having an open upper end and an open lower end. An inflatable ring is affixed about the open upper end. In some embodiments, the inflatable ring is removably securable to the open upper end of the elongated sheath. An inflation tube is in fluid communication with an interior volume of the inflatable ring. A syringe valve is affixed to a distal end of the inflation tube, wherein the syringe valve operably connects to a syringe. Upon depression of a plunger of the syringe, air is dispensed into the inflatable ring.

公开(公告)号：US20050227345A1

公开(公告)日：2005-10-13

申请号：US11014621

申请日：2004-12-15

申请人： Marvin Whiteley ,  Kimberly Lee ,  E. Greenberg ,  Ute Muh

发明人： Marvin Whiteley ,  Kimberly Lee ,  E. Greenberg ,  Ute Muh

IPC分类号： C07K14/21 ,  C12N1/21 ,  C07H21/04 ,  C12N15/74

CPC分类号： C07K14/21

摘要： The invention provides methods for identifying a modulator of quorum sensing signaling in bacteria, and for identifying a quorum sensing controlled gene in bacteria. In addition, the invention provides quorum sensing controlled genetic loci in Pseudomas aeruginosa. Novel indicator strains and vectors for engineering the strains for use in the method of the invention are also provided.

公开(公告)号：US20240033120A1

公开(公告)日：2024-02-01

申请号：US18485027

申请日：2023-10-11

申请人： Kimberly Lee

发明人： Kimberly Lee

IPC分类号： A61F5/453 ,  A61F5/44

CPC分类号： A61F5/453 ,  A61F5/4405

摘要： An external catheter system is provided. The external catheter system includes an elongated sheath having an open upper end and an open lower end and a check valve. An inflatable ring is affixed about the open upper end. In some embodiments, the inflatable ring is removably securable to the open upper end of the elongated sheath. An inflation tube is in fluid communication with an interior volume of the inflatable ring. A syringe valve is affixed to a distal end of the inflation tube, wherein the syringe valve operably connects to a syringe. Upon depression of a plunger of the syringe, air is dispensed into the inflatable ring. The check valve has a generally conical shape and is inserted into the elongated sheath and is disposed before the drainage tube and sits within the elongated sheath.

公开(公告)号：US20210307953A1

公开(公告)日：2021-10-07

申请号：US17216824

申请日：2021-03-30

申请人： Kimberly Lee

发明人： Kimberly Lee

IPC分类号： A61F5/451 ,  A61F5/44 ,  A61M1/00

摘要： An external catheter system is provided. The system includes an elongated sheath having an open upper end and an open lower end. An inflatable ring is affixed about the open upper end. In some embodiments, the inflatable ring is removably securable to the open upper end of the elongated sheath. An inflation tube is in fluid communication with an interior volume of the inflatable ring. A syringe valve is affixed to a distal end of the inflation tube, wherein the syringe valve operably connects to a syringe. Upon depression of a plunger of the syringe, air is dispensed into the inflatable ring.

公开(公告)号：US20090263832A1

公开(公告)日：2009-10-22

申请号：US12086609

申请日：2006-11-29

申请人： Roberto Polakiewicz ,  Ting-Lei Gu ,  Valerie Goss ,  Kimberly Lee

发明人： Roberto Polakiewicz ,  Ting-Lei Gu ,  Valerie Goss ,  Kimberly Lee

IPC分类号： G01N33/573 ,  C12Q1/48 ,  C07K16/18 ,  C12N5/16 ,  C12N5/18

CPC分类号： G01N33/57426

摘要： The invention discloses nearly 123 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Leukemia, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: protein kinases, adaptor/scaffold proteins, phosphatase/phospholipases, G proteins/GTPase activating proteins/guanine nucleotide exchange factors, cellular metabolism enzymes, DNA binding proteins, cytoskeletal proteins, cell cycle regulation proteins, proteases, RNA binding proteins, transcription proteins, translation initiation complex proteins, transferases, ubiquitin conjugating system proteins, vesicle proteins, actin binding proteins, apoptosis proteins, chemokine proteins, enzyme proteins extra cellular matrix proteins, helicases, hydrolases, immunoglobin superfamily proteins, inhibitor proteins, isomerases, ligases, lipid binding proteins, methyltransferases, motor proteins, receptor proteins, and chaperone proteins.

摘要翻译： 本发明公开了在信号转导蛋白中识别的近123个新的磷酸化位点和人类白血病的途径，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白，如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：蛋白激酶，衔接子/支架蛋白，磷酸酶/磷脂酶，G蛋白/ GTPase激活蛋白/鸟嘌呤核苷酸交换因子，细胞代谢酶，DNA结合蛋白，细胞骨架蛋白，细胞 循环调节蛋白，蛋白酶，RNA结合蛋白，转录蛋白，翻译起始复合蛋白​​，转移酶，泛素缀合系统蛋白，囊泡蛋白，肌动蛋白结合蛋白，凋亡蛋白，趋化因子蛋白，酶蛋白超细胞基质蛋白，解旋酶，水解酶，免疫球蛋白 超家族蛋白，抑制蛋白，异构酶，连接酶，脂质结合蛋白，甲基转移酶，运动蛋白，受体蛋白和伴侣蛋白。