公开(公告)号：US20130011875A1

公开(公告)日：2013-01-10

申请号：US13503707

申请日：2010-10-25

申请人： Michael Meehl ,  Heping Lin ,  Byung-Kwon Choi

发明人： Michael Meehl ,  Heping Lin ,  Byung-Kwon Choi

IPC分类号： C12N1/19 ,  C12P21/00

CPC分类号： C07K14/39 ,  C12N15/67 ,  C12N15/81 ,  C12P21/005 ,  C12P21/02

摘要： The present invention is related to methods and for producing higher titers of recombinant protein in a modified yeast host cell, for example Pichia pastoris, wherein the modified yeast cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified yeast host cell of the same species. In particular embodiments vacuolar sorting activity is reduced or eliminated by deletion or disruption of a gene encoding Vps10 or a Vps10 homolog. The invention is also related to the modified yeast cells which are modified in accordance with the methods disclosed herein.

摘要翻译： 本发明涉及在修饰的酵母宿主细胞例如巴斯德毕赤酵母中产生较高滴度的重组蛋白的方法，其中所述修饰的酵母细胞相对于未修饰的酵母宿主细胞缺乏液泡分选活性或具有降低的液泡分选活性 相同的物种 在具体实施方案中，通过缺失或破坏编码Vps10或Vps10同源物的基因来减少或消除液泡分选活性。 本发明还涉及根据本文公开的方法修饰的修饰的酵母细胞。

公开(公告)号：US08715963B2

公开(公告)日：2014-05-06

申请号：US13579972

申请日：2011-02-23

申请人： Natarajan Sethuraman ,  Byung-Kwon Choi ,  Bianka Prinz ,  Michael Meehl ,  Terrance Stadheim

发明人： Natarajan Sethuraman ,  Byung-Kwon Choi ,  Bianka Prinz ,  Michael Meehl ,  Terrance Stadheim

IPC分类号： C12P21/06 ,  C12P21/04 ,  C12P1/02 ,  C12N15/74 ,  C12N5/07 ,  C12N1/00

CPC分类号： C07K16/2863 ,  C07K14/44 ,  C07K16/1027 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/41 ,  C12N15/81 ,  C12P21/005

摘要： Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex. The method is useful for both producing therapeutic glycoproteins with increased N-glycosylation site occupancy in lower eukaryote cells such as yeast and filamentous fungi and in higher eukaryote cells such as plant and insect cells and mammalian cells.

摘要翻译： 描述了增加如本文所述修饰的重组宿主细胞中产生的治疗性糖蛋白的N-糖基化位点占有率的方法，并且与在重组宿主细胞中产生的治疗性糖蛋白的N-糖基化位点占据相比，进行遗传工程改造以表达糖蛋白 未经本文所述修饰。 特别地，该方法提供过表达异源单亚基寡糖基转移酶的重组宿主细胞，其在特定实施方案中能够功能性地抑制酵母寡糖转移酶（OTase）复合物的至少一种必需蛋白质的突变的致死表型，例如 ，利什曼原虫主要STT3D蛋白，在表达编码内源性OTase复合物的宿主细胞基因的存在下。 该方法对于在低等真核生物细胞如酵母和丝状真菌以及高等真核细胞如植物和昆虫细胞和哺乳动物细胞中产生具有增加的N-糖基化位点占有的治疗性糖蛋白是有用的。

公开(公告)号：US08507224B2

公开(公告)日：2013-08-13

申请号：US13057807

申请日：2009-08-10

申请人： Byung-Kwon Choi

发明人： Byung-Kwon Choi

IPC分类号： C12P21/00 ,  C12N1/15 ,  C12N1/19

CPC分类号： C07K16/00 ,  C12N15/815 ,  C12P21/005

摘要： Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.

摘要翻译： 描述了内源或异源Ca2 + ATP酶过表达的低等真核生物宿主细胞。 还描述了其中钙网蛋白和/或ERp57蛋白过表达的低等真核细胞宿主细胞。 这些宿主细胞可用于产生具有降低的O-糖基化的重组糖蛋白。

公开(公告)号：US07890446B2

公开(公告)日：2011-02-15

申请号：US11852047

申请日：2007-09-07

申请人： Joon Koo Kim ,  Kwang-Choon Kim ,  Byung-Kwon Choi

发明人： Joon Koo Kim ,  Kwang-Choon Kim ,  Byung-Kwon Choi

IPC分类号： G06N5/00

CPC分类号： G06N3/004 ,  G06F8/65 ,  G06N3/126

摘要： A software robot apparatus (sobot apparatus) with a genetic code is disclosed. The sobot apparatus includes an application module, an artificial animated object engine, a sobot protocol module, an update agent, an update protocol module, a network interface, a sobot player, a script engine, a player database, a repository interface, a sobot graphic/audio interface, a 3D engine, a system software, a memory device, a display part, and an audio output part.

摘要翻译： 公开了具有遗传密码的软件机器人装置（sobot装置）。 sobot装置包括应用模块，人造动画对象引擎，sobot协议模块，更新代理，更新协议模块，网络接口，sobot玩家，脚本引擎，玩家数据库，存储库界面，sobot 图形/音频接口，3D引擎，系统软件，存储设备，显示部分和音频输出部分。

公开(公告)号：US20100035867A1

公开(公告)日：2010-02-11

申请号：US12307150

申请日：2007-07-06

申请人： Richard L. Guerrant ,  Alexander Y. Kots ,  Ferid Murad ,  Byung-Kwon Choi

发明人： Richard L. Guerrant ,  Alexander Y. Kots ,  Ferid Murad ,  Byung-Kwon Choi

IPC分类号： A61K31/5415 ,  A61K31/519

CPC分类号： A61K31/519 ,  Y02A50/471 ,  Y02A50/473

摘要： We disclose a method of inhibiting activity of adenylyl cyclase or guanylyl cyclase in a mammal by administering to the mammal an amount of a composition effective to inhibit the activity, wherein the composition contains at least one compound selected from the group consisting of structural formulae (Ia) and (Ib) and salts thereof, wherein R1 is —H or has the structure —C(═O)R8; R2 is ═O or has the structure —OC(═O)R9; and R3, R4, R5, R6, and R7 are each independently selected from the group consisting of —H, —NO2, formula (I), -halogen, —OC(═O)R9, —OR9, —OH, —R8OH, —CH3, —OC(═O)CH2Ph, formulae (II), (III), (IV), —OPh, —CF3, —R8, —C(═O)OR9, -Ph, —R8Ph, formulae (V), (VI), (VII), (VIII), (IX), (X), (XI), (XII), (XIII), (XIV), (XV), (XVI), (XVII), (XVIII), (XIX), (XX), and (XXI), wherein each R8 is independently a linear or branched hydrocarbon group having from 1 to 4 carbon atoms and each R9 is independently a hydrocarbon group having from 1 to 2 carbon atoms. Administering the composition can be used to treat a disease in a mammal mediated by activity of adenylyl cyclase or guanylyl cyclase and effected by a toxin produced by a pathogenic organism or to reduce cyclic AMP or cyclic GMP levels in a mammal in need of reduction thereof. The composition can also be administered to mammalian cells in vitro. The above methods of inhibiting activity of adenylyl cyclase or guanylyl cyclase and treating diseases via such inhibition can be effective without prolonged treatment, have reversible effects, have low or no toxicity, are highly potent, are unlikely to have side effects, do not act on purinergic receptors, or can negate pathogenic toxins independently of whether the pathogenic organism survives.

摘要翻译： 我们公开了通过向哺乳动物施用一定量的有效抑制活性的组合物来抑制腺苷酸环化酶或鸟苷酸环化酶的活性的方法，其中组合物含有至少一种选自结构式（Ia ）和（Ib）及其盐，其中R 1是-H或具有-C（-O）R 8的结构; R2是-O或具有-OC（-O）R9的结构; 并且R 3，R 4，R 5，R 6和R 7各自独立地选自-H，-NO 2，式（I）， - 卤素，-OC（-O）R 9，-OR 9，-OH，-R 8 OH ，-CH 3，-OC（-O）CH 2 Ph，式（II），（III），（IV），-OPh，-CF 3，-R 8，-C（ - ）OR 9，-Ph，-R 8 Ph， （ⅩⅢ），（ⅩⅣ），（ⅩⅤ），（ⅩⅥ），（ⅩⅦ），（ⅩⅥ），（ⅩⅥ） ，（XVIII），（XIX），（XX）和（XXI），其中每个R 8独立地为具有1至4个碳原子的直链或支链烃基，并且每个R 9独立地为具有1至2个碳原子的烃基 原子 施用组合物可用于治疗由腺苷酸环化酶或鸟苷酸环化酶的活性介导的哺乳动物疾病，并且由病原生物产生的毒素影响，或减少需要还原的哺乳动物中的环AMP或环GMP水平。 组合物也可以在体外施用于哺乳动物细胞。 上述抑制腺苷酸环化酶或鸟苷酸环化酶的活性和通过这种抑制作用治疗疾病的方法可以在没有延长治疗的情况下有效，具有可逆作用，具有低毒性或无毒性，高度有效，不会产生副作用，不起作用 嘌呤能受体，或者可以否定致病性毒素，而不管病原菌是否存活。

公开(公告)号：US20080299616A1

公开(公告)日：2008-12-04

申请号：US11658113

申请日：2005-07-22

申请人： Byung-Kwon Choi

发明人： Byung-Kwon Choi

IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/19

CPC分类号： C07H21/04 ,  C12N9/88 ,  C12N15/80 ,  C12Y203/03009

摘要： The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, Pichia pastoris. The present invention further relates to the expression of heterologous proteins which have been introduced into a P. pastoris host cell and can be expressed under the regulation of the MLS1 5′ regulatory promoter and MLS1 3′regulatory terminator regions. The MLS1 promoter is repressed in media containing glucose and derepressed under glucose starvation conditions or when acetate is present.

摘要翻译： 本发明涉及从酵母毕赤酵母（Pichia pastoris）分离苹果酸合酶1基因（MLS1）的DNA调节区。 本发明还涉及已经被引入巴斯德毕赤酵母宿主细胞中的异源蛋白质的表达，并且可以在MLS5'调节启动子和MLS1 3调控终止区的调节下表达。 MLS1启动子在含葡萄糖的培养基中被抑制并在葡萄糖饥饿条件下或当存在乙酸盐时除去。

公开(公告)号：US20060271238A1

公开(公告)日：2006-11-30

申请号：US11395768

申请日：2006-03-31

申请人： Byung-Kwon Choi ,  Hyun-Sik Shim ,  Joon-Koo Kim

发明人： Byung-Kwon Choi ,  Hyun-Sik Shim ,  Joon-Koo Kim

IPC分类号： G06F19/00

CPC分类号： B25J9/1689 ,  G05B2219/40174

摘要： Provided are a network-based robot control system and a robot velocity control method in the network-based robot control system. A client calculates a robot control velocity according to its reception state of video data frames captured by a robot, generates a robot control message including the calculated robot control velocity, and transmits the robot control message to the robot. The robot then changes its velocity according to the robot control velocity included in the received robot control message. In this way, the velocity of the robot is controlled according to the video data reception state of the client, thereby allowing a user to easily control the robot regardless of the performance of the client.

摘要翻译： 提供了一种基于网络的机器人控制系统和基于网络的机器人控制系统中的机器人速度控制方法。 客户端根据机器人捕获的视频数据帧的接收状态来计算机器人控制速度，生成包含所计算的机器人控制速度的机器人控制消息，并将机器人控制消息发送给机器人。 然后机器人根据包含在接收的机器人控制消息中的机器人控制速度改变其速度。 以这种方式，根据客户端的视频数据接收状态来控制机器人的速度，从而允许用户容易地控制机器人，而不管客户端的性能如何。