Bacillus thuringiensis toxins active against scarab pests
    33.
    发明授权
    Bacillus thuringiensis toxins active against scarab pests 失效
    苏云金芽孢杆菌毒素对抗虫虫有活性

    公开(公告)号:US5554534A

    公开(公告)日:1996-09-10

    申请号:US315468

    申请日:1994-09-30

    摘要: Certain isolates of Bacillus thuringiensis (B.t.) have been found to have activity against scarab pests. These isolates are designated B.t. PS86B1, B.t. PS43F and B.t. PS50C. These isolates, or transformed hosts containing the gene expressing a scarab-active toxin obtained from the isolates, can be used to control scarab-active pests, e.g., masked chafer, Cyclocephala sp., June beetle, Cotinis sp., northern masked chafer, Cyclocephala borealis, Japanese beetle, Popillia japonica, and Pasadena masked chafer, Cyclocephala pasadenae, in various environments.

    摘要翻译: 已经发现某些苏云金芽孢杆菌(B.t.)的分离株具有抗金龟虫的活性。 这些分离株被命名为B.t. PS86B1,B.t. PS43F和B.t. PS50C。 这些分离物或含有表达从分离物获得的金丝雀毒素活性毒素的基因的转化宿主可以用于控制金龟子活性害虫,例如掩蔽的胎膜缠绕,Cyclocephala sp。,June甲虫,Cotinis sp。,北部掩蔽的胎圈包布, Cyclocephala borealis,日本甲虫,Popillia japonica和Pasadena在各种环境中掩盖了潮湿发髻,Cyclocephala pasadenae。

    Modified Cry35 proteins
    37.
    发明授权
    Modified Cry35 proteins 有权
    修饰的Cry35蛋白

    公开(公告)号:US07939651B2

    公开(公告)日:2011-05-10

    申请号:US12715744

    申请日:2010-03-02

    IPC分类号: C07H19/16 C07K14/00 C12N15/09

    CPC分类号: C07K14/325

    摘要: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

    摘要翻译: 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维(3D)结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸,以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫(包括根虫)的方法。 本发明的修饰蛋白包括涉及交换的片段,结构域和基序的嵌合毒素,如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。

    Modified Cry35 proteins having altered protease stability
    39.
    发明授权
    Modified Cry35 proteins having altered protease stability 有权
    具有改变的蛋白酶稳定性的修饰的Cry35蛋白

    公开(公告)号:US07737263B2

    公开(公告)日:2010-06-15

    申请号:US11943814

    申请日:2007-11-21

    IPC分类号: C07H19/16 C07K14/00 C12N15/09

    CPC分类号: C07K14/325

    摘要: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

    摘要翻译: 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维(3D)结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸,以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫(包括根虫)的方法。 本发明的修饰蛋白包括涉及交换的片段,结构域和基序的嵌合毒素,如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。