摘要:
Noven B.t. genes encoding toxins active against nematode pests have been cloned. The DNA encoding the B.t. toxin can be used to transform various hosts to express the B.t. toxin.
摘要:
Novel B.t. genes encoding toxins active against nematode pests have been cloned. The DNA encoding the B.t. toxin can be used to transform various hosts to express the B.t. toxin.
摘要:
Certain isolates of Bacillus thuringiensis (B.t.) have been found to have activity against scarab pests. These isolates are designated B.t. PS86B1, B.t. PS43F and B.t. PS50C. These isolates, or transformed hosts containing the gene expressing a scarab-active toxin obtained from the isolates, can be used to control scarab-active pests, e.g., masked chafer, Cyclocephala sp., June beetle, Cotinis sp., northern masked chafer, Cyclocephala borealis, Japanese beetle, Popillia japonica, and Pasadena masked chafer, Cyclocephala pasadenae, in various environments.
摘要:
This invention concerns novel gene probes which can be used to identify DNA from Bacillus thuringiensis microbes which encode protein endotoxins. The invention probes greatly facilitate the search for useful microbes hosting genes which encode toxins.
摘要:
A novel B.t. gene encoding a toxin which is toxic to coleopteran insects has been cloned from a coleopteran-active B. thurningiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control coleopteran insects in various environments.
摘要:
This invention relates to the field of lignin degradation. More specifically the invention relates to methods for selecting and isolating microorganism from nature that are capable of degrading lignin, processes for cloning a gene segment from such an organism, and methods of using the enzyme product of the gene segment to provide valuable chemical feedstocks, methanol and the like from a lignin source material.
摘要:
This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.
摘要:
The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.
摘要:
This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.
摘要:
The subject invention provides KB59A4-6 pesticidal proteins and preferred methods of using these proteins to control lepidoteran pests. This invention provides Bacillus thuringiensis isolate KB59A4-6.