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公开(公告)号：US07871805B2
公开(公告)日：2011-01-18
申请号：US12351177
申请日：2009-01-09
IPC分类号： C12N9/10
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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公开(公告)号：US07494794B2
公开(公告)日：2009-02-24
申请号：US11694540
申请日：2007-03-30
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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公开(公告)号：US20060062999A1
公开(公告)日：2006-03-23
申请号：US11081537
申请日：2005-03-17
IPC分类号： B32B5/16
CPC分类号： C09K11/7706 , C01G49/009 , C01P2004/03 , C01P2004/62 , C01P2006/42 , C01P2006/60 , C09K11/06 , G01N33/5434 , G01N33/582 , Y10T428/2982 , Y10T428/2991 , Y10T428/2993
摘要： The present invention provides composite particles which have magnetism and simultaneously emit fluorescence with a variety of wavelengths, and which are suitable for use in the fields of biology, biochemistry or the like. The composite particles of the present invention comprise ferromagnetic iron oxide particles, fluorescent pigment particles and silica, and have an average particle size of 1 to 10 μm, a coercive force of 2.39 to 11.94 kA/m (30 to 150 oersted), saturation magnetization of 0.5 to 40 A.m2/kg (0.5 to 40 emu/g). The peak value of the wavelength of fluorescence from the composite particle is in the range of 350 to 750 nm, when the composite particle is excited by light with a wavelength of 250 to 600 nm.
摘要翻译： 本发明提供具有磁性并且同时发出具有各种波长的荧光并且适用于生物学，生物化学等领域的复合颗粒。 本发明的复合颗粒包含铁磁性氧化铁颗粒，荧光颜料颗粒和二氧化硅，平均粒径为1-10μm，矫顽力为2.39至11.94kA / m（30至150奥斯特），饱和磁化强度 为0.5〜40Am 2 / kg（0.5〜40emu / g）。 当复合颗粒被波长为250至600nm的光激发时，来自复合颗粒的荧光波长的峰值在350至750nm的范围内。
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34.发明授权Protein having .alpha.-glucosidase activity, DNA having genetic information thereof, and production of .alpha.-glucosidase 失效具有α-葡糖苷酶活性的蛋白质，具有遗传信息的DNA和α-葡糖苷酶的产生
公开(公告)号：US5795766A
公开(公告)日：1998-08-18
申请号：US611361
申请日：1996-03-06
申请人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
发明人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
CPC分类号： C12N9/2414 , C12N9/2408
摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No. 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.
摘要翻译： 具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1的本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。 根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。
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35.发明授权DNA encoding .alpha.-glucosidase and method of producing same by genetic engineering 失效编码α-葡萄糖苷酶的DNA及其基因工程生产方法
公开(公告)号：US5550046A
公开(公告)日：1996-08-27
申请号：US39777
申请日：1993-03-22
申请人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
发明人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
CPC分类号： C12N9/2414 , C12N9/2408
摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No: 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.
摘要翻译： 具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1中本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。 根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。
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36.发明授权公开(公告)号：US08728771B2
公开(公告)日：2014-05-20
申请号：US13139205
申请日：2009-12-10
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
CPC分类号： C12N9/80 , C12P13/04 , C12P13/06 , C12P13/222 , C12P41/007
摘要： The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.
摘要翻译： 本发明提供了一种L-琥珀酰基酰化酶，其由以下组成：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 或（d）由氨基酸序列组成的蛋白质，其中一个或多个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。 该酶能够产生空间庞大的非天然氨基酸如L-叔亮氨酸等，其可用作药物的中间体。
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公开(公告)号：US20080020426A1
公开(公告)日：2008-01-24
申请号：US11694540
申请日：2007-03-30
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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38.发明申请Device for separation of biological components, and method of separation of biological components using the device 审中-公开用于分离生物成分的装置，以及使用该装置分离生物成分的方法公开(公告)号：US20060186055A1
公开(公告)日：2006-08-24
申请号：US10564563
申请日：2004-07-13
IPC分类号： C02F1/48
CPC分类号： B03C1/01
摘要： The present invention provides a device relating to separation (extraction, purification) of a biological component such as nucleic acid, protein and the like from a liquid sample containing the biological component, and, as a method, a device for separating a biological component, which contains magnetically responsive particles and a chip obtained by adhering a pair of substrates, which contains one or multiple grooves formed on at least one surface thereof, with the groove(s) placed inside, and a method of separating a biological component from a liquid sample, which uses this device, and includes the following steps (a)-(d): (a) a step of holding the device such that the adhesion surface of the pair of substrates is about perpendicular to the horizontal direction, (b) a step of adsorbing the biological component to magnetically responsive particles by contacting the magnetically responsive particles with the liquid sample containing the biological component, (c) a step of separating the magnetically responsive particles containing the biological component adsorbed thereto from the liquid sample, and (d) a step of separating the biological component from the magnetically responsive particles.
摘要翻译： 本发明提供了从含有生物成分的液体样品中分离（提取，纯化）生物成分如核酸，蛋白质等的装置，作为分离生物成分的装置， 其包含磁响应性颗粒和通过将包含形成在其至少一个表面上的一个或多个凹槽的一对基板与设置在内部的凹槽粘附而获得的芯片，以及将生物组分与液体分离的方法 （a） - （d）：（a）保持所述装置的步骤，使得所述一对基板的粘附表面垂直于水平方向，（b）使用该装置的样品，并且包括以下步骤 通过使磁响应性颗粒与含有生物成分的液体样品接触，将生物成分吸附到磁响应性颗粒上的步骤，（c）分离步骤 g含有从液体样品吸附到其上的生物成分的磁响应颗粒，和（d）将生物成分与磁响应颗粒分离的步骤。
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