公开(公告)号：US10894949B2

公开(公告)日：2021-01-19

申请号：US16058938

申请日：2018-08-08

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Li Zhong

IPC: A61K48/00 ,  A61K38/17 ,  C12N15/113 ,  C12N15/86 ,  C12N7/00 ,  C12N15/864

Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

公开(公告)号：US10829783B2

公开(公告)日：2020-11-10

申请号：US15367708

申请日：2016-12-02

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Jun Xie

IPC: C12N15/86 ,  C12N15/113 ,  C12N7/00

Abstract: Some aspects of this invention provide nucleic acid constructs for transgene expression. Some aspects of this invention provide multicistronic nucleic acid constructs, for example, comprising an expression cassette encoding a hairpin RNA and a reporter expression cassette. Some aspects of this invention provide nucleic acid constructs comprising two or more self-complementary nucleic acid sequences, for example, hairpin RNA encoding nucleic acid sequences and AAV inverse terminal repeats. Methods for the use of the constructs in therapy and research are also provided.

公开(公告)号：US10689420B2

公开(公告)日：2020-06-23

申请号：US16019636

申请日：2018-06-27

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Terence Flotte ,  Jun Xie

IPC: C12N15/00 ,  C12N7/00 ,  A61K39/23 ,  C07K14/005 ,  C12N15/86 ,  A61K31/7088 ,  A61K35/76 ,  A61K48/00

Abstract: The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

公开(公告)号：US20190194688A1

公开(公告)日：2019-06-27

申请号：US16234819

申请日：2018-12-28

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Hongwei Zhang ,  Hongyan Wang ,  Zuoshang Xu

IPC: C12N15/86 ,  A61K31/713 ,  A61K38/50 ,  C12N7/00 ,  A61K48/00 ,  C12N9/80 ,  C12N15/864 ,  C12N15/113

CPC classification number: C12N15/86 ,  A61K31/713 ,  A61K38/50 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  A61P25/00 ,  C12N7/00 ,  C12N9/80 ,  C12N15/1137 ,  C12N15/635 ,  C12N15/8645 ,  C12N2310/141 ,  C12N2750/14133 ,  C12N2750/14141 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2750/14162 ,  C12N2810/10 ,  C12N2840/007 ,  C12Y305/01015

Abstract: The invention in some aspects relates to recombinant adeno-associated viruses useful for targeting transgenes to CNS tissue, and compositions comprising the same, and methods of use thereof. In some aspects, the invention provides methods and compositions for treating CNS-related disorders.

公开(公告)号：US20190085301A1

公开(公告)日：2019-03-21

申请号：US16058938

申请日：2018-08-08

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Li Zhong

IPC: C12N7/00 ,  A61K38/17 ,  A61K48/00 ,  C12N15/113 ,  C12N15/86

Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

公开(公告)号：US20180362592A1

公开(公告)日：2018-12-20

申请号：US16019636

申请日：2018-06-27

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Terence Flotte ,  Jun Xie

IPC: C07K14/005 ,  C12N15/86 ,  C12N7/00 ,  A61K31/7088 ,  A61K35/76

Abstract: The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

公开(公告)号：US20180311380A1

公开(公告)日：2018-11-01

申请号：US15769953

申请日：2016-10-21

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Jianzhong Ai ,  Hong Li ,  Qiang Wei

IPC: A61K48/00 ,  C12N15/86 ,  A61P35/00

CPC classification number: C07K14/005 ,  A61K9/0019 ,  A61K9/0034 ,  A61K38/1709 ,  A61K48/00 ,  C07K14/075 ,  C07K14/47 ,  C12N5/10 ,  C12N15/86 ,  C12N2750/10041 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14145

Abstract: The disclosure relates to compositions and methods for rAAV-mediated delivery of a transgene to a subject. In some embodiments, the rAAV transduces the prostate tissue of a subject. In some embodiments, the methods are useful for treatment of prostate disease (e.g., prostatitis, BPH, prostate cancer).

公开(公告)号：US20180214576A1

公开(公告)日：2018-08-02

申请号：US15747801

申请日：2016-07-28

Applicant: University of Massachusetts

Inventor： Katherine A. Fitzgerald ,  Stefan A. Schattgen ,  Guangping Gao

IPC: A61K48/00 ,  C12N15/63 ,  C12N9/22 ,  A61K9/00 ,  A61P37/02

CPC classification number: A61K48/0058 ,  A61K9/007 ,  A61K38/46 ,  A61P37/02 ,  C12N9/22 ,  C12N15/63 ,  C12N2750/14143

Abstract: The disclosure relates to compositions and methods for AAV-mediated delivery of a nuclease to a subject. In some embodiments, the nuclease is DNAse I. In some embodiments, the methods are useful for treatment of lung-associated diseases.

公开(公告)号：US09284357B2

公开(公告)日：2016-03-15

申请号：US14246560

申请日：2014-04-07

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Terence Flotte ,  Jun Xie

IPC: A61K39/23 ,  A61K39/235 ,  C12N15/00 ,  C12P21/06 ,  C07K14/005 ,  C12N15/86

CPC classification number: C07K14/005 ,  A61K31/7088 ,  A61K35/76 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14145

Abstract: The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

Abstract translation: 本发明在某些方面涉及具有不同组织靶向能力的重组腺相关病毒。 在一些方面，本发明涉及使用重组腺相关病毒的基因转移方法。 在一些方面，本发明涉及分离的AAV衣壳蛋白和编码其的分离的核酸。

公开(公告)号：US20240243975A1

公开(公告)日：2024-07-18

申请号：US18432467

申请日：2024-02-05

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Phillip Tai ,  Elisabet C. Mandon ,  Jianzhong Ai

IPC: H04L41/12 ,  H04L41/22

CPC classification number: H04L41/12 ,  H04L41/22

Abstract: In some aspects, the disclosure provides recombinant AAV and nucleic acid constructs having novel inverted terminal repeats (ITRs), cap, and/or rep genes. In some aspects, the disclosure relates to gene transfer methods using rAAVs described herein.