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192 条记录 (耗时 0.029 秒)

    Haemophilus adhesin protein
    32.
    发明授权
    Haemophilus adhesin protein 失效
    嗜血杆菌粘附素蛋白

    公开(公告)号：US06218147B1

    公开(公告)日：2001-04-17

    申请号：US09456287

    申请日：1999-12-08

    申请人： Clifford A. Lingwood

    发明人： Clifford A. Lingwood

    IPC分类号： C12N1509

    CPC分类号： C07K14/285 ,  A61K39/102

    摘要： An adhesin protein which binds specifically to phosphatidylethanolamine (PE), gangliotriaosylceramide (Gg3) and gangliotetraosylceramide (Gg4) has been isolated and purified from H. influenzae. Also provided are immunogenic compositions and methods of protecting susceptible mammals from diseases caused by bacterial pathogens having the adhesin as a surface protein.

    摘要翻译： 已经从流感嗜血杆菌中分离和纯化了与磷脂酰乙醇胺（PE），神经节苷脂神经酰胺（Gg3）和神经节四糖基神经酰胺（Gg4）特异性结合的粘附素蛋白。 还提供了免疫原性组合物和保护易感染哺乳动物免受具有粘附素作为表面蛋白质的细菌病原体引起的疾病的方法。

    UDP-galactose: &bgr;-N-acetyl-glucosamine &bgr;1,3galactosyltransferases, &bgr;3Gal-T5
    34.
    发明授权
    UDP-galactose: &bgr;-N-acetyl-glucosamine &bgr;1,3galactosyltransferases, &bgr;3Gal-T5 失效
    UDP-半乳糖：β-N-乙酰葡糖胺β1,3半乳糖转移酶，β3Gal-T5

    公开(公告)号：US06800468B1

    公开(公告)日：2004-10-05

    申请号：US09831630

    申请日：2001-05-10

    申请人： Henrik Clausen ,  Margarida Amado

    发明人： Henrik Clausen ,  Margarida Amado

    IPC分类号： C12N1509

    CPC分类号： C12N9/1051 ,  C07K2319/00

    摘要： A novel gene defining a novel enzyme in the UDP-D-galactose: &bgr;-N-acetylglucosamine/&bgr;-N-acetylgalactosamine &bgr;1,3galactosyltransferase family, termed &bgr;3Gal-T5, with unique enzymatic properties is disclosed. The enzymatic activity of &bgr;3Gal-T5 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding &bgr;3Gal-T5 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting &bgr;3Gal-T5 activity, as well as cloning and expression vectors including such DNA, cells tranfected with the vectors, and recombinant methods for providing &bgr;3Gal-T5. The enzyme &bgr;3Gal-T5 and &bgr;3Gal-T5-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of &bgr;3Gal-T5. Further, the invention discloses methods of obtaining &bgr;1,3galactosyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active &bgr;3Gal-T5 protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active &bgr;3Gal-T5 protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification of DNA sequence variations in the &bgr;3Gal-T5 gene by isolating DNA from a patient, amplifying &bgr;3Gal-T5-coding exons by PCR, and detecting the presence of DNA sequence variation, are disclosed.

    摘要翻译： 公开了一种在UDP-D-半乳糖：β-N-乙酰氨基葡萄糖/β-N-乙酰半乳糖胺β1,3半乳糖转移酶家族中定义新型酶的新基因，称为β3Gal-T5，具有独特的酶学性质。 β3Gal-T5的酶活性显示与以前鉴定的该基因家族酶的活性不同。 本发明公开了通过氨基酸缺失，取代或插入显示β3Gal-T5活性的编码β3Gal-T5及其衍生物的分离的DNA分子和DNA构建体，以及包含这些DNA的克隆和表达载体，用载体转染的细胞，以及 提供beta3Gal-T5的重组方法。 公开了酶β3Gal-T5和β3Gal-T5-活性衍生物，特别是包含β3Gal-T5的催化活性结构域的可溶性衍生物。 此外，本发明公开了通过使用酶活性β3Gal-T5蛋白或其融合蛋白或通过使用包含编码酶活性β3Gal-T5蛋白的DNA的载体稳定转染的细胞获得β1,3半乳糖基糖基化糖，糖肽或糖蛋白的方法 作为重组生产这种糖肽或糖蛋白的表达系统。 还公开了通过从患者中分离DNA，通过PCR扩增β3Gal-T5编码外显子并检测DNA序列变异的存在来鉴定β3Gal-T5基因中的DNA序列变异的方法。

    Agrobacterium tumefaciens rpoA gene
    36.
    发明授权
    Agrobacterium tumefaciens rpoA gene 失效
    根癌土壤杆菌rpoA基因

    公开(公告)号：US06764837B1

    公开(公告)日：2004-07-20

    申请号：US09958940

    申请日：2002-01-16

    申请人： Shouguang Jin ,  Scott M. Lohrke ,  Konstantin Severinov ,  Marie Chow

    发明人： Shouguang Jin ,  Scott M. Lohrke ,  Konstantin Severinov ,  Marie Chow

    IPC分类号： C12N1509

    CPC分类号： C07H21/00 ,  C12N9/1247 ,  C12N15/67

    摘要： The present invention is directed to a method for expression of at least one heterologous gene in a host cell comprising transforming a host cell with at least one nucleic acid construct comprising a complete &agr; subunit of an RNA polymerase or a portion thereof of a hybrid nucleic acid containing a portion of the &agr; subunit of an RNA polymerase obtained from the same genus as the heterologous gene, and transforming the host cell, with at least one heterologous gene; and culturing the transformed host cell. The present invention further is directed to nucleic acid molecules used in the present method, vectors containing these nucleic acid molecules, and host cells containing the nucleic acid molecules. The nucleic acid encoding the &agr; subunit of an Agrobacterium RNA polymerase and the corresponding amino acid sequence and portions thereof is disclosed.

    摘要翻译： 本发明涉及用于在宿主细胞中表达至少一种异源基因的方法，包括用至少一种核酸构建体转化宿主细胞，所述核酸构建体包含RNA聚合酶的完整α亚基或其混合核酸的一部分 含有与异源基因相同属的RNA聚合酶的α亚基的一部分，并用至少一个异源基因转化宿主细胞; 并培养转化的宿主细胞。 本发明还涉及本方法中使用的核酸分子，含有这些核酸分子的载体和含有核酸分子的宿主细胞。 公开了编码农杆菌RNA聚合酶的α亚基的核酸和相应的氨基酸序列及其部分。

    Site-specific cell perforation technique
    37.
    发明授权
    Site-specific cell perforation technique 失效
    位点特异性细胞穿孔技术

    公开(公告)号：US06753171B2

    公开(公告)日：2004-06-22

    申请号：US09623970

    申请日：2000-12-28

    申请人： Isao Karube ,  Takashi Saitoh

    发明人： Isao Karube ,  Takashi Saitoh

    IPC分类号： C12N1509

    CPC分类号： C12N15/89 ,  C12M35/00 ,  C12N13/00

    摘要： A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.

    摘要翻译： 开发了除物理剪切力之外控制膜变性反应的技术。 例如，本发明提供了通过使诸如光的刺激与由刺激激活的化合物反应而使特定部位的膜破裂的方法，其中反应发生在诸如生物膜的膜上。 还提供了通过本方法获得的膜结构，例如其中特异性位点已被破坏的细胞。 使用这种膜结构也可以引入基因等物质。 还提供了用于破坏特定部位的膜的破坏薄膜的部件。 因此，本发明能够例如使用通常难以用于穿透细胞膜的微电极，显微操纵器和显微注射器的构成的薄膜穿透。

    Maize H3C4 promoter combined with the first intron of rice actin, chimeric gene comprising it and transformed plant
    38.
    发明授权
    Maize H3C4 promoter combined with the first intron of rice actin, chimeric gene comprising it and transformed plant 失效
    玉米H3C4启动子与水稻肌动蛋白的第一内含子，包含它的嵌合基因和转化的植物组合

    公开(公告)号：US06750378B2

    公开(公告)日：2004-06-15

    申请号：US09037531

    申请日：1998-03-10

    申请人： Richard Derose ,  Georges Freyssinet

    发明人： Richard Derose ,  Georges Freyssinet

    IPC分类号： C12N1509

    CPC分类号： C12N15/8222 ,  C12N15/8216 ,  C12N15/8274

    摘要： The present invention relates to a DNA sequence, a 5′ regulatory element allowing the expression of a heterologous gene in a plant cell from a monocotyledonous plant, characterized in that it comprises, in the direction of transcription, a first DNA sequence, which is a functional fragment of the sequence of the maize H3C4 promoter, and a second DNA sequence, which is a functional fragment of the sequence of the first intron of rice actin. The invention also relates to a chimeric gene comprising the said DNA sequence and the plants transformed with the said gene.

    摘要翻译： 本发明涉及一种DNA序列，一种允许从单子叶植物植物细胞中表达异源基因的5'调节元件，其特征在于其在转录方向上包含第一DNA序列，其为 玉米H3C4启动子序列的功能片段和第二DNA序列，其是水稻肌动蛋白的第一内含子的序列的功能片段。本发明还涉及包含所述DNA序列和转化的植物的嵌合基因 与该基因。

    Modification of PI-TA gene conferring fungal disease resistance to plants
    39.
    发明授权
    Modification of PI-TA gene conferring fungal disease resistance to plants 失效
    修饰PI-TA基因，赋予植物真菌抗性

    公开(公告)号：US06743969B2

    公开(公告)日：2004-06-01

    申请号：US09993170

    申请日：2001-11-14

    申请人： Gregory Bryan ,  Barbara Valent

    发明人： Gregory Bryan ,  Barbara Valent

    IPC分类号： C12N1509

    CPC分类号： C12N15/8282 ,  C07K14/415

    摘要： This invention concerns the preparation and use of an isolated nucleic acid fragments in order to confer a resistance gene mediated defense response in plants against a fungus comprising in its genome virulent and/or avirulent AVR-Pita alleles. Chimeric genes incorporating such fragments and suitable regulatory sequences can be used to create transgenic plants which can produce a resistance gene mediated defense response against a fungus comprising in its genome virulent and/or avirulent AVR-Pita alleles.

    摘要翻译： 本发明涉及分离的核酸片段的制备和使用，以便在植物中赋予抗性基因介导的针对包含其基因组毒性和/或无毒AVR-Pita等位基因的真菌的防御反应。 可以使用掺入这种片段和合适的调节序列的嵌合基因来产生转基因植物，其可以产生针对包含其基因组毒性和/或无毒AVR-Pita等位基因的真菌的抗性基因介导的防御反应。