公开(公告)号：US08617888B2

公开(公告)日：2013-12-31

申请号：US12842448

申请日：2010-07-23

申请人： Shouguang Jin ,  Candace Bichsel

发明人： Shouguang Jin ,  Candace Bichsel

IPC分类号： C12N5/00 ,  C12N5/02 ,  C07H21/04 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00

CPC分类号： C12R1/385 ,  C07K14/21 ,  C07K14/4702 ,  C12N5/0696 ,  C12N15/87 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605

摘要： A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.

摘要翻译： 已经开发了经修饰的铜绿假单胞菌III型分泌系统，其有效地将选择的蛋白质递送到宿主细胞中。 在一个实例中，功能核Cre重组酶被注射到胚胎干（ES）细胞中，并且可以用于诱导多能干（iPS）细胞。 这种体外谱系定向分化的方法预防插入突变，并提供了选择的干细胞更新和基于细胞的疗法的途径。

公开(公告)号：US06764837B1

公开(公告)日：2004-07-20

申请号：US09958940

申请日：2002-01-16

申请人： Shouguang Jin ,  Scott M. Lohrke ,  Konstantin Severinov ,  Marie Chow

发明人： Shouguang Jin ,  Scott M. Lohrke ,  Konstantin Severinov ,  Marie Chow

IPC分类号： C12N1509

CPC分类号： C07H21/00 ,  C12N9/1247 ,  C12N15/67

摘要： The present invention is directed to a method for expression of at least one heterologous gene in a host cell comprising transforming a host cell with at least one nucleic acid construct comprising a complete &agr; subunit of an RNA polymerase or a portion thereof of a hybrid nucleic acid containing a portion of the &agr; subunit of an RNA polymerase obtained from the same genus as the heterologous gene, and transforming the host cell, with at least one heterologous gene; and culturing the transformed host cell. The present invention further is directed to nucleic acid molecules used in the present method, vectors containing these nucleic acid molecules, and host cells containing the nucleic acid molecules. The nucleic acid encoding the &agr; subunit of an Agrobacterium RNA polymerase and the corresponding amino acid sequence and portions thereof is disclosed.

摘要翻译： 本发明涉及用于在宿主细胞中表达至少一种异源基因的方法，包括用至少一种核酸构建体转化宿主细胞，所述核酸构建体包含RNA聚合酶的完整α亚基或其混合核酸的一部分 含有与异源基因相同属的RNA聚合酶的α亚基的一部分，并用至少一个异源基因转化宿主细胞; 并培养转化的宿主细胞。 本发明还涉及本方法中使用的核酸分子，含有这些核酸分子的载体和含有核酸分子的宿主细胞。 公开了编码农杆菌RNA聚合酶的α亚基的核酸和相应的氨基酸序列及其部分。

公开(公告)号：US20120021517A1

公开(公告)日：2012-01-26

申请号：US12842448

申请日：2010-07-23

申请人： SHOUGUANG JIN ,  CANDACE BICHSEL

发明人： SHOUGUANG JIN ,  CANDACE BICHSEL

IPC分类号： C12N5/00 ,  C12N1/21

CPC分类号： C12R1/385 ,  C07K14/21 ,  C07K14/4702 ,  C12N5/0696 ,  C12N15/87 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605

摘要： A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.

摘要翻译： 已经开发了经修饰的铜绿假单胞菌III型分泌系统，其有效地将选择的蛋白质递送到宿主细胞中。 在一个实例中，功能核Cre重组酶被注射到胚胎干（ES）细胞中，并且可以用于诱导多能干（iPS）细胞。 这种体外谱系定向分化的方法预防插入突变，并提供了选择的干细胞更新和基于细胞的疗法的途径。

公开(公告)号：US07271005B2

公开(公告)日：2007-09-18

申请号：US10425241

申请日：2003-04-29

申请人： Shouguang Jin ,  Unhwan Ha

发明人： Shouguang Jin ,  Unhwan Ha

IPC分类号： C12N15/74 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/21

摘要： Nucleic acids and polypeptides involved in regulation of membrane permeability in bacteria are disclosed. Also disclosed are methods of increasing sensitivity to antibiotics in multi-drug resistant bacteria by increasing expression of PprA or PprB proteins in bacterial cells, and methods for identifying compounds that modulate PprA/PprB expression.

摘要翻译： 公开了涉及细菌膜通透性调节的核酸和多肽。 还公开了通过增加细菌细胞中PprA或PprB蛋白的表达以及用于鉴定调节PprA / PprB表达的化合物的方法来增加多重耐药细菌中对抗生素的敏感性的方法。