公开(公告)号：US06410337B1

公开(公告)日：2002-06-25

申请号：US08933181

申请日：1997-09-18

申请人： Terry Brady ,  Michael F. Corsello

发明人： Terry Brady ,  Michael F. Corsello

IPC分类号： G01N3386

CPC分类号： G01N33/86 ,  G01N2800/52

摘要： A method for determining platelet activation by utilizing numeric counts of platelets before a sample of platelets has been activated and after the activatable platelets are activated with a platelet activation agonist and using the difference between such counts as an indication of the platelet activity of the sample. There is also disclosed a method for using the electronic impedance cell counting technique for determining platelet activation wherein EDTA is used as a preservative by counting the platelets in an EDTA preserved sample using an electronic impedance cell counting technique and subtracting from that number the number of platelets remaining after the activatable platelets in a second sample have been activated with a platelet agonist in the absence of EDTA and using that difference as a measure of platelet activity.

摘要翻译： 一种在血小板样品被激活之前和在可活化的血小板用血小板活化激动剂活化并使用这些计数之间的差异作为样品的血小板活性指示之间的差异的情况下通过利用血小板的数值计算来确定血小板活化的方法。 还公开了一种使用电子阻抗细胞计数技术来确定血小板活化的方法，其中EDTA用作防腐剂，通过使用电子阻抗细胞计数技术对EDTA保存的样品中的血小板进行计数，并从该数目减去血小板数 在第二样品中的可激活血小板已经在不存在EDTA的情况下用血小板激动剂活化并且使用该差异作为血小板活性的量度之后剩余。

公开(公告)号：US06187594B1

公开(公告)日：2001-02-13

申请号：US09215167

申请日：1998-12-18

申请人： Michael Kraus ,  Carsten Schelp ,  Andreas Wiegand

发明人： Michael Kraus ,  Carsten Schelp ,  Andreas Wiegand

IPC分类号： G01N3386

CPC分类号： G01N33/86

摘要： The invention relates to a method and to a diagnostic agent for detecting hemostasis disturbances, wherein, as a consequence of blood platelet aggregation, clot formation and/or clot dissolution, substances are brought to a distance from each other which permits or prevents an interaction, in particular an energy transfer, between the substances, and the extent of the interaction is measured.

摘要翻译： 本发明涉及一种用于检测止血障碍的方法和诊断剂，其中，由于血小板聚集，凝块形成和/或凝块溶解的结果，使物质彼此间隔一段距离，这允许或阻止相互作用， 特别是物质之间的能量转移，并且测量相互作用的程度。

公开(公告)号：US06743398B2

公开(公告)日：2004-06-01

申请号：US10127461

申请日：2002-04-23

申请人： Teruaki Itoh

发明人： Teruaki Itoh

IPC分类号： G01N3386

CPC分类号： G01N33/491 ,  G01N33/86

摘要： A serum/clot separation surface determination apparatus includes a test tube formed of a non-magnetic material containing a blood sample which is separated into a serum and a clot by a silicon separating medium, a detection coil fitted on an outer surface of the test tube, a measurement signal supply unit which supplies a measurement signal having a given frequency to the detection coil, a drive control unit which variably controls a relative position between the detection coil and the test tube in a longitudinal direction of the test tube by moving at least one of the detection coil and the test tube, a signal level detector which detects a level of the measurement signal that varies with the relative position, and a determination unit which determines a position of a sample separation surface obtained by the silicon separating medium, based on the level of the detected measurement signal.

摘要翻译： 血清/凝块分离表面测定装置包括由含有通过硅分离介质分离成血清和凝块的血液样品的非磁性材料形成的试管，安装在试管外表面上的检测线圈 测量信号提供单元，其向检测线圈提供具有给定频率的测量信号;驱动控制单元，其通过至少移动来可变地控制检测线圈和试管在试管的纵向上的相对位置 检测线圈和试管中的一个，检测随相对位置变化的测量信号的电平的信号电平检测器，以及确定由硅分离介质获得的样品分离表面的位置的确定单元 在检测到的测量信号的电平上。

公开(公告)号：US06699718B1

公开(公告)日：2004-03-02

申请号：US09645786

申请日：2000-08-24

申请人： Berndt B. Bruegger

发明人： Berndt B. Bruegger

IPC分类号： G01N3386

CPC分类号： G01N33/86 ,  G01N2800/52 ,  Y10T436/10 ,  Y10T436/108331

摘要： The invention is a method, reagent and test cartridge for the determination of the clotting time of a blood sample by means of a reagent containing tissue factor and a sulfatide. In an alternative embodiment, the reagent may contain tissue factor and at least one of the group consisting of a phosphatide and a sulfatide. This invention is preferably used to monitor the effectiveness of heparin therapy in patients that have been administered low to moderate heparin doses to achieve blood heparin levels from 0 to about 3 U/mL, and may also be used for determining clotting time at higher heparin levels of up to about 6 U/mL.

摘要翻译： 本发明是用于通过含有组织因子和硫苷脂的试剂测定血液样品的凝血时间的方法，试剂和测试盒。 在替代实施方案中，试剂可以含有组织因子和由磷脂和硫苷脂组成的组中的至少一种。 本发明优选用于监测已经施用低至中等肝素剂量以实现0至约3U / mL的血液肝素水平的患者中肝素治疗的有效性，并且还可用于确定较高肝素水平的凝血时间 高达约6U / mL。

公开(公告)号：US06645768B1

公开(公告)日：2003-11-11

申请号：US09698589

申请日：2000-10-27

申请人： Liliana Tejidor ,  Trevor Baglin ,  Hema Doobay ,  Roger Luddington ,  Timothy J. Fischer

发明人： Liliana Tejidor ,  Trevor Baglin ,  Hema Doobay ,  Roger Luddington ,  Timothy J. Fischer

IPC分类号： G01N3386

CPC分类号： G01N33/86 ,  Y10T436/10

摘要： A reagent and kit are disclosed for determining if a patient is hypercoagulable, hypocoagulable or normal. The test involves providing a test sample from the patient and initiating coagulation in the sample in the presence of an activator, which is added to the sample in an amount which will result in intrinsic tenase-dependent fibrin. Then the formation of the intrinsic tenase-dependent fibrin polymerization is monitored over time so as to derive a time-dependent profile, with the results of the fibrin polymerization monitoring determining whether the patient is hypercoagulable, normal or hypocoagulable. The coagulation activator is added in an amount that triggers a thrombin explosion that is dependent on the propagation phase and amplification pathways. In this way, a single assay can assess the hemostatic potential of a sample.

摘要翻译： 公开了一种试剂和试剂盒，用于确定患者是否是高凝状态，低凝或正常。 该试验涉及提供来自患者的测试样品，并在活化剂存在下引发样品中的凝血，其以一定量导致固有的依赖于tenase的纤维蛋白加入到样品中。 然后，随着时间的推移监测内在的tenase依赖性纤维蛋白聚合的形成，从而导出时间依赖性分布，纤维蛋白聚合监测的结果确定患者是否是高凝状态，正常或低凝状态。 加入的凝血活化剂的量会触发依赖于繁殖期和扩增途径的凝血酶爆发。 以这种方式，单个测定可以评估样品的止血电位。

公开(公告)号：US06632679B1

公开(公告)日：2003-10-14

申请号：US09501118

申请日：2000-02-09

申请人： Enzo Breda

发明人： Enzo Breda

IPC分类号： G01N3386

CPC分类号： G01N15/05

摘要： Method and apparatus to determine the speed of sedimentation of blood and other parameters connected thereto, said method being carried out by detecting the development over time of the optical density, or absorbance, of a sample of blood, said sample being sent in the form of a flow inside a capillary container (12), said detection being made in correspondence with a point of said capillary container (12) and the relative data acquired being processed to obtain said speed of sedimentation and said connected parameters, said method providing to instantly interrupt the flow of the blood sample flowing inside said capillary container (12), in order to determine a thickening of the red cells in said blood sample and their consequent sedimentation, said detection being made substantially simultaneously with said instant interruption.

摘要翻译： 确定血液沉淀速度和与其相关的其他参数的方法和装置，所述方法通过检测血液样品的光密度或吸光度随时间的变化来进行，所述样品以 毛细管容器内的流动（ 12 ），所述检测与所述毛细管容器的一个点（ 12 >），并且获取的相对数据被获得以获得所述沉降速度和所述连接的参数，所述方法提供立即中断在所述毛细管容器内流动的血液样品的流动（ 12 PDAT>），为了确定所述血液样品中红细胞的增厚及其随后的沉淀，所述检测基本上与所述即时中断同时进行。

公开(公告)号：US06632678B2

公开(公告)日：2003-10-14

申请号：US09753974

申请日：2001-01-03

申请人： Jennifer C. Aiken ,  Jon H. Henderson ,  Barbara A. DeBiase

发明人： Jennifer C. Aiken ,  Jon H. Henderson ,  Barbara A. DeBiase

IPC分类号： G01N3386

CPC分类号： G01N33/86 ,  G01N2333/8117 ,  G01N2400/40

摘要： A coagulation test for determining the activated clotting time (ACT) of blood in the presence of heparin that produces test results that are substantially insensitive to the drug aprotinin. The activator is formulated to be a combination of celite and bentonite. The ACT results obtained with this formulation are similar to celite ACT tests on heparinized blood while simultaneously being unaffected by aprotinin. Additionally, a method for quantifying the aprotinin effect of different ACT formulations is disclosed.

摘要翻译： 用于确定在肝素存在下的血液的活化凝血时间（ACT）的凝血试验，其产生对药物抑肽酶基本不敏感的测试结果。 活化剂配制成硅藻土和膨润土的组合。 用该制剂获得的ACT结果与肝素化血液的硅藻土ACT测试相似，同时不受抑肽酶的影响。 另外，公开了一种用于量化不同ACT制剂的抑肽酶作用的方法

公开(公告)号：US06566140B2

公开(公告)日：2003-05-20

申请号：US09844209

申请日：2001-04-27

申请人： Kenneth G. Mann ,  Mathew D. Rand ,  Kevin M. Cawthern

发明人： Kenneth G. Mann ,  Mathew D. Rand ,  Kevin M. Cawthern

IPC分类号： G01N3386

CPC分类号： G01N33/86 ,  G01N2333/811

摘要： The present invention provides methods for inhibiting blood clotting. In general, the methods include adding corn trypsin inhibitor (CTI) to blood or a blood product in an amount sufficient to inhibit the clotting. The CTI can be used alone or in combination with other anti-coagulants. In one aspect, the invention features plasma clotting assays featuring substantially prolonged clotting times. Clotting assays using whole or minimally altered blood are also provided. Further provided are methods for storing blood or blood products at low temperature with the CTI.

公开(公告)号：US06537819B2

公开(公告)日：2003-03-25

申请号：US09845222

申请日：2001-04-30

申请人： Eli Cohen ,  Peter R. Delmenico ,  Gabriel Raviv ,  William R. George ,  John A. Lake

发明人： Eli Cohen ,  Peter R. Delmenico ,  Gabriel Raviv ,  William R. George ,  John A. Lake

IPC分类号： G01N3386

CPC分类号： G01N11/162 ,  G01N11/167 ,  G01N33/4905

摘要： A method and apparatus are provided for measuring hemostasis. The apparatus includes a torque sensing column having a torque sensing element and a drive ring disposed around a body of the column and in registration with the column so as to allow rotation of the drive ring around a longitudinal axis of the column. The apparatus further includes a first guide shaft rigidly secured to the drive ring, the guide shaft extending parallel to the longitudinal axis of the column and a cup holder movably attached to the guide shaft, allowing the cup holder to move parallel to the longitudinal axis of the column. The apparatus also includes a sample cup adapted to engage the cup holder on a outer surface and the torque sensing element of the torque sensing column on an inner surface.

摘要翻译： 提供了一种用于测量止血的方法和装置。 该装置包括具有转矩检测元件的转矩感测柱和设置在柱体上并与柱对准的驱动环，以便允许驱动环围绕柱的纵向轴线旋转。 该装置还包括刚性地固定到驱动环的第一引导轴，平行于柱的纵向轴线延伸的引导轴和可移动地连接到引导轴的杯架，允许杯架平行于纵向轴线 列。 该装置还包括适于在外表面上接合杯架的样品杯和内表面上的转矩感测塔的转矩感测元件。

公开(公告)号：US06475801B1

公开(公告)日：2002-11-05

申请号：US09385249

申请日：2000-05-12

申请人： Mikiko Nishizaki ,  Yasumasa Akai ,  Sayaka Nakamura

发明人： Mikiko Nishizaki ,  Yasumasa Akai ,  Sayaka Nakamura

IPC分类号： G01N3386

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/108331

摘要： An anticoagulant for blood cell counting containing at least two kinds of the following antiplatelet agents: theophyllines, adenosines and dipyridamoles. The anticoagulant also contains citric acid, an alkali metal salt thereof or a mixture of citric acid and an alkali metal salt thereof.

摘要翻译： 含有至少两种以下抗血小板药物的血细胞计数抗凝剂：茶碱，腺苷和双嘧达莫。 抗凝剂还含有柠檬酸，其碱金属盐或柠檬酸及其碱金属盐的混合物。