公开(公告)号：US20190309317A1

公开(公告)日：2019-10-10

申请号：US16471960

申请日：2017-12-20

Applicant: Altria Client Services LLC

Inventor： Andrew Carl Adams ,  Marcos Fernando de Godoy Lusso ,  Sreepriya Pramod ,  Dongmei Xu

IPC: C12N15/82 ,  A24B3/12 ,  A24B15/20 ,  A24B13/00

Abstract: The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US10435700B2

公开(公告)日：2019-10-08

申请号：US14875928

申请日：2015-10-06

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan Kudithipudi ,  Yanxin Shen ,  Dongmei Xu ,  Jesse Frederick ,  Jaemo Yang

IPC: C12N15/82 ,  C12N15/31 ,  C07K14/415

Abstract: This disclosure provides a number of sequences involved in axillary bud growth in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences or transgenes of such sequences, and tobacco products made from tobacco leaf harvested from such plants.

公开(公告)号：US10306915B2

公开(公告)日：2019-06-04

申请号：US14263530

申请日：2014-04-28

Applicant: Altria Client Services LLC

Inventor： John Kotrola ,  Ujwala Warek ,  James Arthur Strickland ,  Elisabeth Miller ,  Dongmei Xu

IPC: A24B3/14 ,  A24B15/12 ,  A24B15/28

Abstract: Methods of making reconstituted tobacco include contacting a mixture including tobacco pulp with one or more fluid streams including one or more peroxides (e.g., hydrogen peroxide), recovering one or more fluids from the tobacco pulp mixture, and reusing the recovered fluids in at least one subsequent tobacco pulp contacting step. In some cases, methods provided herein can recover one or more fluids including one or more peroxides, such that the at least one subsequent tobacco pulp contacting step includes contacting tobacco pulp with a fluid including one or more peroxides.

公开(公告)号：US20170298376A1

公开(公告)日：2017-10-19

申请号：US15486841

申请日：2017-04-13

Applicant: Altria Client Services LLC

Inventor： Yanxin Shen ,  Dongmei Xu

IPC: C12N15/82 ,  A24B13/00 ,  A01H1/06 ,  A01H5/12

CPC classification number: C12N15/8261 ,  A01H5/12 ,  A24B13/00 ,  C07K14/415 ,  C12N9/0073 ,  C12N15/8218 ,  C12N15/825 ,  C12N15/8269 ,  C12Y114/13122 ,  Y02A40/146

Abstract: This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.

公开(公告)号：US12270037B2

公开(公告)日：2025-04-08

申请号：US17766786

申请日：2020-10-09

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan Kudithipudi ,  Rajanikanth Govindarajulu ,  Nazmul Bhuiyan ,  Raja Payyavula ,  Yanxin Shen ,  Dongmei Xu

IPC: C12N15/82 ,  C07K14/415 ,  C12N15/113

Abstract: The present disclosure provides composition and methods for improving leaf quality of low-alkaloid tobacco plants. Also provided are the identification and genetic engineering of target genes (Leaf Quality Genes) for producing tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US12234469B2

公开(公告)日：2025-02-25

申请号：US17337161

申请日：2021-06-02

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Sreepriya Pramod ,  Marcos Fernando de Godoy Lusso ,  Jesse Frederick ,  Andrew Adams ,  Dongmei Xu

IPC: C12N15/82

Abstract: The present disclosure provides tobacco having genetic modification(s) in a group of ERF genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20250051794A1

公开(公告)日：2025-02-13

申请号：US18928836

申请日：2024-10-28

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: C12N15/82 ,  A24B3/12 ,  A24B15/20 ,  A61K38/00 ,  C12N9/88 ,  C12N15/10 ,  C12N15/66

Abstract: This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.

公开(公告)号：US12193474B2

公开(公告)日：2025-01-14

申请号：US17892239

申请日：2022-08-22

Applicant: Altria Client Services LLC

Inventor： Yahya Karga ,  James Powell ,  Dongmei Xu ,  James A. Strickland ,  Ujwala Warek

IPC: A24B15/22 ,  A24B13/00 ,  A24B15/10 ,  A24B15/18 ,  A24B15/28

Abstract: A stabilizing process for tobacco and tobacco products includes sterilizing the tobacco by applying an electron beam treatment to the tobacco. The process may also include treating the tobacco. The treating the tobacco includes pre-conditioning the tobacco, pasteurizing the tobacco, curing the tobacco, fermenting the tobacco, or combinations thereof. The method may also include forming a smokeless tobacco product containing the tobacco.

公开(公告)号：US11999961B2

公开(公告)日：2024-06-04

申请号：US17830072

申请日：2022-06-01

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland ,  Yanxin Shen

IPC: C12N15/82 ,  A01H6/82 ,  A24B13/00 ,  A24B15/10 ,  A24B15/24

CPC classification number: C12N15/8243 ,  A01H6/823 ,  A24B13/00 ,  A24B15/10 ,  A24B15/245 ,  C12N15/8251 ,  C12Y402/01091

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), increase phenylalanine, or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US11873500B2

公开(公告)日：2024-01-16

申请号：US17683227

申请日：2022-02-28

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan Kudithipudi ,  Alec J. Hayes ,  Robert Frank Hart ,  Yanxin Shen ,  Jesse Frederick ,  Dongmei Xu

IPC: A01H6/82 ,  A01H5/12 ,  A01H1/06 ,  A24B13/00 ,  C12N15/82 ,  A01H1/04 ,  C12Q1/6895

CPC classification number: C12N15/8261 ,  A01H1/045 ,  A01H1/06 ,  A01H5/12 ,  A01H6/823 ,  A24B13/00 ,  C12N15/8218 ,  C12N15/8243 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: Provided herein are genetic markers and a coding sequence associated with a low- or ultra-low anatabine trait in tobacco.