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41.发明授权Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same 有权突变体EGIII纤维素酶，编码这种EGIII组合物的DNA及其获得方法公开(公告)号：US06187732B1
公开(公告)日：2001-02-13
申请号：US09146770
申请日：1998-09-03
申请人： Timothy Fowler , Colin Mitchinson
发明人： Timothy Fowler , Colin Mitchinson
IPC分类号： C11D3386
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： The present invention relates to variant EGIII cellulases which have improved stability and/or performance. The variant cellulases have replacements at sensitive residues to improve stability and/or performance.
摘要翻译： 本发明涉及具有改善的稳定性和/或性能的变体EGIII纤维素酶。 变体纤维素酶在敏感残基中具有替代物以改善稳定性和/或性能。
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公开(公告)号：US08765440B2
公开(公告)日：2014-07-01
申请号：US13072516
申请日：2011-03-25
IPC分类号： C12N9/42 , C12P19/00 , C12P21/06 , C12P19/34 , C12N15/00 , C12N1/00 , C12N1/20 , C07H21/04 , C07K1/00 , C11D3/02 , C11D3/00
CPC分类号： C12Y302/01091 , C12N9/2437
摘要： Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
摘要翻译： 公开了许多Hypocrea jecorina Cel7A（以前的里氏木霉纤维二糖水解酶I或CBH1）的同系物和变体，编码它们的核酸及其制备方法。 同系物和变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代和/或缺失。
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公开(公告)号：US08637294B2
公开(公告)日：2014-01-28
申请号：US11728219
申请日：2007-03-22
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译： 本文描述的是H.jecorina CBH I，Cel7酶的变体。 本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。
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44.发明申请USE OF CELLULASE AND GLUCOAMYLASE TO IMPROVE ETHANOL YIELDS FROM FERMENTATION 审中-公开纤维素酶和谷氨酰胺酶从发酵中提高乙醇含量公开(公告)号：US20120276593A1
公开(公告)日：2012-11-01
申请号：US13458597
申请日：2012-04-27
申请人： Mian Li , Colin Mitchinson
发明人： Mian Li , Colin Mitchinson
摘要： An improved saccharification process comprises the use of a glucoamylase and at least one cellulase. The improved saccharification process results in improved yields of fermentations products, such as ethanol. In one embodiment, the improved saccharification process results in an increased yield of up to 0.5% to 1% ethanol using commercially available cellulases. Also provided are improved simultaneous saccharification and fermentation (SSF) processes, and compositions comprising a liquefied starch slurry, a glucoamylase, and a cellulase.
摘要翻译： 改进的糖化方法包括使用葡糖淀粉酶和至少一种纤维素酶。 改进的糖化过程导致发酵产物如乙醇的产率提高。 在一个实施方案中，改进的糖化过程导致使用市售的纤维素酶增加高达0.5％至1％乙醇的产量。 还提供了改进的同时糖化和发酵（SSF）方法，以及包含液化淀粉浆料，葡糖淀粉酶和纤维素酶的组合物。
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公开(公告)号：US20120064579A1
公开(公告)日：2012-03-15
申请号：US13264905
申请日：2010-04-30
申请人： Aaron Kelley , Chuanbin Liu , Colin Mitchinson
发明人： Aaron Kelley , Chuanbin Liu , Colin Mitchinson
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/02 , C12P19/12 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091
摘要： This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hemicellulolytic activity.
摘要翻译： 本公开涉及用于从细胞培养物，特别是可以优先增加由木聚糖酶基因启动子序列控制下的基因产生的蛋白质表达的培养物组分和条件改进蛋白质生产的方法。 改进的方法可用于生产具有增强的木聚糖酶和半纤维素分解活性的酶组合物。
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公开(公告)号：US20110294165A1
公开(公告)日：2011-12-01
申请号：US13092086
申请日：2011-04-21
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
摘要翻译： 披露的是灰腐质霉Cel7A（CBH1.1），H.jecorina CBH1变体或嗜热链球菌CBH1的变体，编码它们的核酸及其生产方法。 变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代。
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公开(公告)号：US08008056B2
公开(公告)日：2011-08-30
申请号：US11317110
申请日：2005-12-22
申请人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Brad Kelemen , Robert Caldwell , Pauline Teunissen
发明人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Brad Kelemen , Robert Caldwell , Pauline Teunissen
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译： 本文描述了H.cecorina CBH2，Cel6A酶的变体。 本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。
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公开(公告)号：US20110177561A1
公开(公告)日：2011-07-21
申请号：US13072516
申请日：2011-03-25
CPC分类号： C12Y302/01091 , C12N9/2437
摘要： Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
摘要翻译： 公开了许多Hypocrea jecorina Cel7A（以前的里氏木霉纤维二糖水解酶I或CBH1）的同系物和变体，编码它们的核酸及其制备方法。 同系物和变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代和/或缺失。
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公开(公告)号：US07951571B2
公开(公告)日：2011-05-31
申请号：US12259843
申请日：2008-10-28
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
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公开(公告)号：US07951570B2
公开(公告)日：2011-05-31
申请号：US12250227
申请日：2008-10-13
CPC分类号： C12Y302/01091 , C12N9/2437
摘要： Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
摘要翻译： 公开了许多Hypocrea jecorina Cel7A（以前的里氏木霉纤维二糖水解酶I或CBH1）的同系物和变体，编码它们的核酸及其制备方法。 同系物和变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代和/或缺失。
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