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公开(公告)号：US08715997B2
公开(公告)日：2014-05-06
申请号：US13178227
申请日：2011-07-07
申请人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
发明人： Wolfgang Aehle , Frits Goedegebuur , Lydia Dankmeyer , Colin Mitchinson , Paulien Neefe , Bradley Kelemen , Robert Caldwell , Pauline Teunissen
IPC分类号： C12N9/42 , C12N9/14 , C12P21/06 , C12P19/34 , C12N15/00 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译： 本文描述了H.cecorina CBH2，Cel6A酶的变体。 本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。
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公开(公告)号：US08377659B2
公开(公告)日：2013-02-19
申请号：US13092068
申请日：2011-04-21
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
摘要翻译： 披露的是灰腐质霉Cel7A（CBH1.1），H.jecorina CBH1变体或嗜热链球菌CBH1的变体，编码它们的核酸及其生产方法。 变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代。
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公开(公告)号：US20120270270A1
公开(公告)日：2012-10-25
申请号：US13542482
申请日：2012-07-05
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
摘要翻译： 披露的是灰腐质霉Cel7A（CBH1.1），H.jecorina CBH1变体或嗜热链球菌CBH1的变体，编码它们的核酸及其生产方法。 变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代。
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公开(公告)号：US08202704B2
公开(公告)日：2012-06-19
申请号：US13029919
申请日：2011-02-17
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译： 本文描述了从里氏木霉分离的新基因序列。 描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。 序列CIP1和CIP2含有纤维素结合结构域。 这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。
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公开(公告)号：US20110294164A1
公开(公告)日：2011-12-01
申请号：US13092068
申请日：2011-04-21
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
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公开(公告)号：US20110251073A1
公开(公告)日：2011-10-13
申请号：US12996538
申请日：2009-06-03
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C11D3/38681 , C11D11/0017 , C12N9/52 , C12Y304/21062
摘要： The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein. The present invention also provides variant subtilisins suitable for various uses.
摘要翻译： 本发明提供蛋白质工程方法。 具体地说，本发明提供了使用位点评估库来设计优化蛋白质的两个或多个性质的文库的方法。 本发明还提供适用于各种用途的变体枯草杆菌蛋白酶。
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7.发明申请OVER EXPRESSION OF FOLDASES AND CHAPERONES IMPROVES PROTEIN PRODUCTION 有权过度表达FOLDASES和CHAPERONES改进蛋白质生产公开(公告)号：US20100144013A1
公开(公告)日：2010-06-10
申请号：US12531030
申请日：2008-03-21
CPC分类号： C12N9/6483 , C07K14/37 , C12P21/02 , C12Y304/23004
摘要： The present teachings provide methods for increasing protein secretion, e.g., chymosin in filamentous fungi by co-expressing certain chaperone(s) and/or foldase(s). The present teachings also provide filamentous fungi containing certain chaperone(s) and/or foldase(s) and a protein of interest for increased secretion.
摘要翻译： 本教导提供了通过共同表达某些伴侣和/或折叠酶来增加蛋白质分泌的方法，例如丝状真菌中的凝乳酶。 本教导还提供含有某些伴侣和/或折叠酶的丝状真菌和用于增加分泌的目标蛋白质。
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公开(公告)号：US07727754B2
公开(公告)日：2010-06-01
申请号：US11900519
申请日：2007-09-12
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2445 , C12P7/06 , Y02E50/17
摘要： The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl5, and the corresponding BGL5 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL5, recombinant BGL5 proteins and methods for producing the same.
摘要翻译： 本发明提供了一种命名为bgl5的新型的葡糖苷酶核酸序列和相应的BGL5氨基酸序列。 本发明还提供了包含编码BGL5的核酸序列，重组BGL5蛋白的表达载体和宿主细胞及其制备方法。
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公开(公告)号：US07666648B2
公开(公告)日：2010-02-23
申请号：US10555358
申请日：2004-05-28
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry. cip1 cDNA sequence (SEQ ID NO: 1) GACTAGTTCA TAATACAGTA GTTGAGTTCA TAGCAACTTC50 ACTCTCTAGC TGAACAAATT ATCTGCGCAA ACATGGTTCG CCGGACTGCT100 CTGCTGGCCC TTGGGGCTCT CTCAACGCTC TCTATGGCCC AAATCTCAGA150 CGACTTCGAG TCGGGCTGGG ATCAGACTAA ATGGCCCATT TCGGCACCAG200 ACTGTAACCA GGGCGGCACC GTCAGCCTCG ACACCACAGT AGCCCACAGC250 GGCAGCAACT CCATGAAGGT CGTTGGTGGC CCCAATGGCT ACTGTGGACA300 CATCTTCTTC GGCACTACCC AGGTGCCAAC TGGGGATGTA TATGTCAGAG350 CTTGGATTCG GCTTCAGACT GCTCTCGGCA GCAACCACGT CACATTCATC400 ATCATGCCAG ACACCGCTCA GGGAGGGAAG CACCTCCGAA TTGGTGGCCA450 AAGCCAAGTT CTCGACTACA ACCGCGAGTC CGACGATGCC ACTCTTCCGG500 ACCTGTCTCC CAACGGCATT GCCTCCACCG TCACTCTGCC TACCGGCGCG550 TTCCAGTGCT TCGAGTACCA CCTGGGCACT GACGGAACCA TCGAGACGTG600 GCTCAACGGC AGCCTCATCC CGGGCATGAC CGTGGGCCCT GGCGTCGACA650 ATCCAAACGA CGCTGGCTGG ACGAGGGCCA GCTATATTCC GGAGATCACC700 GGTGTCAACT TTGGCTGGGA GGCCTACAGC GGAGACGTCA ACACCGTCTG750 GTTCGACGAC ATCTCGATTG CGTCGACCCG CGTGGGATGC GGCCCCGGCA800 GCCCCGGCGG TCCTGGAAGC TCGACGACTG GGCGTAGCAG CACCTCGGGC850 CCGACGAGCA CTTCGAGGCC AAGCACCACC ATTCCGCCAC CGACTTCCAG900 GACAACGACC GCCACGGGTC CGACTCAGAC ACACTATGGC CAGTGCGGAG1000 GGATTGGTTA CAGCGGGCCT ACGGTCTGCG CGAGCGGCAC GACCTGCCAG1050 GTCCTGAACC CATACTACTC CCAGTGCTTA TAAGGGGATG AGCATGGAGT1100 GAAGTGAAGT GAAGTGGAGA GAGTTGAAGT GGCATTGCGC TCGGCTGGGT1150 AGATAAAAGT CAGCAGCTAT GAATACTCTA TGTGATGCTC ATTGGCGTGT1200 ACGTTTTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA1250 AAAAAAAAAA AAAAAAAAAG GGGGCGGCCG C1271
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公开(公告)号：US20090170181A1
公开(公告)日：2009-07-02
申请号：US12250316
申请日：2008-10-13
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38636 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12N2310/11 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2201/00 , C12Y302/01004 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
摘要翻译： 本发明提供了一种命名为egl7的新型内切葡聚糖酶核酸序列和相应的EGVII氨基酸序列。 本发明还提供了包含编码EGVII的核酸序列，重组EGVII蛋白的表达载体和宿主细胞及其制备方法。
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