Virtual-machine-based application-service provision of front-end versions of back-end applications
    41.
    发明授权
    Virtual-machine-based application-service provision of front-end versions of back-end applications 有权
    基于虚拟机的应用服务提供前端应用的前端版本

    公开(公告)号:US08763005B2

    公开(公告)日:2014-06-24

    申请号:US13259590

    申请日:2009-07-24

    IPC分类号: G06F9/46 G06F9/455

    摘要: Embodiments of the present invention are directed to application-service provision by application-service-provider centers to remote application-service consumers, including small businesses, medium-sized businesses, and other similarly-sized institutions and organizations, as well as to users of individual computers, such as home computers. Rather than executing applications at an application-service-provider center, the applications are instead distributed to remote client sites for execution by virtual machines controlled and managed by an application-service-provider center. Execution of the applications by virtual machines at remote client sites alleviates constraints and bottlenecks associated with communications between client computers and remotely executing applications, while providing management capabilities and security that allow the application-service center to manage application execution as if the remotely executing applications were executing within the application-service center.

    摘要翻译: 本发明的实施例涉及应用服务提供商中心向远程应用服务消费者(包括小型企业,中型企业和其他类似规模的机构和组织)提供的应用服务,以及用户 个人电脑,如家用电脑。 应用程序不是在应用程序服务提供商中心执行应用程序,而是分配到远程客户端站点,以便由应用程序服务提供商中心控制和管理的虚拟机执行。 通过远程客户端站点的虚拟机执行应用程序可减轻与客户端计算机和远程执行应用程序之间的通信相关联的约束和瓶颈,同时提供管理功能和安全性,允许应用程序服务中心管理应用程序执行,就好像远程执行的应用程序 在应用服务中心内执行。

    Method of determining types I, II, III, IV or V or methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample
    43.
    发明授权
    Method of determining types I, II, III, IV or V or methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample 有权
    在生物样品中测定I,II,III,IV或V型或耐甲氧西林金黄色葡萄球菌(MRSA)的方法

    公开(公告)号:US08715936B2

    公开(公告)日:2014-05-06

    申请号:US12930663

    申请日:2011-01-13

    IPC分类号: C12Q1/68 C12P19/34 C07H21/04

    摘要: Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.

    摘要翻译: 公开了用于确定哺乳动物生物样品中耐甲氧西林金黄色葡萄球菌(MRSA)亚型的诊断方法。 方法包括提供哺乳动物的生物样品,对生物样品进行PCR分析,并根据其大小对PCR扩增子进行分析,以确定I型,II型,III型,IV型或V型MRSA, 可能存在于生物样品中。 另外的实施例包括在PCR分析中使用至少一个mecA引物对和/或使用至少一种金黄色葡萄球菌引物对。 进一步披露的是用于筛选MRSA群体的方法以及治疗IV型MRSA阳性的哺乳动物的方法。 还公开了用于测定哺乳动物中MRSA亚型的试剂盒和可用于本发明方法和试剂盒的分离的引物。

    Oligonucleotides Useful in Methods for Detecting and Characterizing Aspergillus fumigatus
    44.
    发明申请
    Oligonucleotides Useful in Methods for Detecting and Characterizing Aspergillus fumigatus 有权
    用于检测和表征烟曲霉的方法中的寡核苷酸

    公开(公告)号:US20130230848A1

    公开(公告)日:2013-09-05

    申请号:US13461122

    申请日:2012-09-17

    IPC分类号: C12Q1/68

    摘要: Methods for using oligonucleotides in the detection of Aspergillus fumigatus are disclosed. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (the cyp51A protein) of Aspergillus fumigatus. The oligonucleotides include primers capable of producing amplicons specific to cyp51A in polymerase chain reactions using nucleic acids isolated from Aspergillus fumigatus as templates. The oligonucleotides also include probes capable of detecting these cyp51A-specific amplicons. The oligonucleotides of the invention also include primers for nucleotide sequencing reactions to determine whether an isolate of Aspergillus fumigatus is more tolerant than wild-type Aspergillus fumigatus to a triazole.

    摘要翻译: 公开了在检测烟曲霉中使用寡核苷酸的方法。 本发明的寡核苷酸具有衍生自编码烟曲霉的细胞色素P45014α-固醇脱甲基酶(cyp51A蛋白)的基因的核苷酸序列。 寡核苷酸包括能够使用从烟曲霉分离的核酸作为模板在聚合酶链反应中产生cyp51A特异性扩增子的引物。 寡核苷酸还包括能够检测这些cyp51A特异性扩增子的探针。 本发明的寡核苷酸还包括用于核苷酸测序反应的引物,以确定烟曲霉分离物是否比野生型烟曲霉对三唑的耐受性更高。

    System and method for integrating a plurality of software applications
    46.
    发明授权
    System and method for integrating a plurality of software applications 有权
    用于集成多个软件应用的系统和方法

    公开(公告)号:US08352875B2

    公开(公告)日:2013-01-08

    申请号:US12620520

    申请日:2009-11-17

    IPC分类号: G06F13/00 G06F15/00

    CPC分类号: G06F9/45512

    摘要: A method for integrating a plurality of existing software applications is disclosed. The method comprises executing the plurality of existing software applications. A composite graphical user interface (GUI) is created containing selected objects. A user's interactions with each of the plurality of existing software applications are recorded to record at least one function from each of the plurality of existing software applications. The at least one recorded function from each of the plurality of existing software applications is assigned to at least one of the selected objects in the composite GUI.

    摘要翻译: 公开了一种用于集成多个现有软件应用的方法。 该方法包括执行多个现有的软件应用。 创建包含所选对象的复合图形用户界面(GUI)。 记录用户与多个现有软件应用中的每一个的交互以记录来自多个现有软件应用中的每一个的至少一个功能。 来自多个现有软件应用程序中的每一个的至少一个记录功能被分配给复合GUI中的所选择的对象中的至少一个。

    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents
    49.
    发明申请
    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents 有权
    与之相关的方法和组合物用于检测和鉴定来自致病因子的不同种类的核酸

    公开(公告)号:US20120064509A1

    公开(公告)日:2012-03-15

    申请号:US13199380

    申请日:2011-08-26

    IPC分类号: C12Q1/70

    CPC分类号: C12Q1/705

    摘要: Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

    摘要翻译: 在本文中描述了用于在单个容器中,例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法,所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物,从而在物种间限定PCR靶区域,并提供对靶区域内的核酸序列特异的第一寡核苷酸探针, 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针,其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记,进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签,从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的,例如,其中感染剂是疱疹病毒科家族的成员。