公开(公告)号：US20080206845A1

公开(公告)日：2008-08-28

申请号：US11709946

申请日：2007-02-23

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: C12M1/36 ,  C12M1/38 ,  C12M3/00

CPC classification number: C12M41/22 ,  C12M23/10 ,  C12M23/48 ,  C12M23/50 ,  C12M41/48

Abstract: A bioreactor analysis system for incubating and analysis of a bioreactive material. The system comprises at least one bioreactor, preferably controlled environment bioreactors. The bioreactor may be held in a sleeve, and multiple sleeves may form a series that moving bioreactors into various storage and interventional positions. At least one interventional assembly interacts with the bioreactor while in the sleeve, and alternately, additional interventional assemblies may interact with the bioreactor while out of the sleeve. A jacket with an access port may surround the bioreactor, which may include a temperature management system. Alternately, a plurality of bioreactors may be joined to a storage array by the cooperation of intrinsic structures in the bioreactors and array. A control system allows for multiple individualized commands to be directed to any one or many of the bioreactors, and may utilize programs resident in the system or in remote locations far from the system.

Abstract translation: 用于孵化和分析生物反应物质的生物反应器分析系统。 该系统包括至少一个生物反应器，优选受控环境的生物反应器。 生物反应器可以保持在套筒中，并且多个套筒可以形成将生物反应器移动到各种存储和介入位置的系列。 至少一个介入组件在套管中与生物反应器相互作用，并且交替地，另外的介入组件可能在与套筒之间的生物反应器相互作用。 具有进入端口的护套可围绕生物反应器，其可以包括温度管理系统。 或者，多个生物反应器可以通过生物反应器和阵列中的固有结构的协作而连接到存储阵列。 控制系统允许将多个单独命令定向到任何一个或多个生物反应器，并且可以利用驻留在系统中或远离系统的远程位置的程序。

公开(公告)号：US07244413B2

公开(公告)日：2007-07-17

申请号：US11301050

申请日：2005-12-12

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61B10/00

CPC classification number: C12Q1/6816 ,  B82Y15/00 ,  C12Q1/6818 ,  G01N33/586 ,  G01N33/588 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  C12Q2563/161 ,  C12Q2563/107 ,  C12Q2523/107

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

Abstract translation: 提供了一种官能化包封的荧光纳米晶体，其包含其中封装有一种或多种荧光纳米晶体的脂质体; 在检测系统中使用官能化封装的荧光纳米晶体; 以及制备官能化包封的荧光纳米晶体的方法。 使用具有结合到其上的亲和分子的官能化包封的荧光纳米晶体的方法包括使官能化的包封的荧光纳米晶体与样品接触，使得在官能化的包封的荧光纳米晶体和亲本分子具有结合特异性的底物之间形成复合物，如果底物是 当下; 将检测系统中的复合物暴露于激发光源，并检测从复合物发射的荧光峰（如果存在）。

公开(公告)号：US20060039991A1

公开(公告)日：2006-02-23

申请号：US11208231

申请日：2005-08-19

Applicant: Alberto Gorrochategui Barrueta ,  Josu Simon Elizundia ,  Emilio Barbera-Guillem

Inventor： Alberto Gorrochategui Barrueta ,  Josu Simon Elizundia ,  Emilio Barbera-Guillem

IPC: A61K35/16

CPC classification number: A61K35/16

Abstract: A biological tissue regenerative agent and method for preparing the same. The agent comprises the compounds that are usually found sequestered within platelets, along with platelet cytosolic components, and serum. The agent is prepared by preparing two quanta of blood. The first is clotted, the cells discarded and the serum retained. The second quantum undergoes concentration and lysis of the platelets therein, followed by recombination of the lysed platelets and platelet internal products with serum to form the agent. In a preferred embodiment, lysis of platelets is accomplished by providing an effective amount of calcium. The avoidance of a platelet release reaction and the presence of alpha-2 macroglobulin in the serum suppresses active TGF-β in the agent. The agent may be further purified and may be frozen or freeze dried for storage.

Abstract translation: 一种生物组织再生剂及其制备方法。 该试剂包括通常发现在血小板内隔离的化合物，以及血小板胞质成分和血清。 该药剂是通过制备两种血液量来制备的。 首先凝结，弃去细胞，保留血清。 第二个量子在其中进行血小板的浓缩和裂解，然后将裂解的血小板和血小板内部产物与血清重组以形成试剂。 在优选的实施方案中，通过提供有效量的钙来完成血小板的溶解。 在血清中避免血小板释放反应和α-2巨球蛋白的存在抑制了药物中的活性TGF-β。 可以进一步纯化试剂并将其冷冻或冷冻干燥以储存。

公开(公告)号：US20050148036A1

公开(公告)日：2005-07-07

申请号：US10997648

申请日：2004-11-24

Applicant: Emilio Barbera-Guillem ,  M. Nelson

Inventor： Emilio Barbera-Guillem ,  M. Nelson

IPC: G01N33/569 ,  C12N5/00 ,  G01N33/574

CPC classification number: G01N33/56972 ,  G01N2800/52

Abstract: A method for screening an individual for a pathological condition comprising a pro-tumor immune response by assaying a clinical sample, obtained from the individual, with a plurality of affinity ligands for detecting and determining an amount of mononuclear cell phenotype. The amount of mononuclear cell phenotype determined in the clinical sample is then compared to a reference value for the mononuclear cell phenotype, wherein a difference in the amount of mononuclear cell phenotype determined from the clinical sample as compared to the reference value comprises an indicator of the presence of a pro-tumor immune response.

Abstract translation: 一种用于通过用多个亲和配体测定从个体获得的临床样品检测和测定单核细胞表型的量来筛选个体的病理状况的方法，其包括前肿瘤免疫应答。 然后将临床样品中确定的单核细胞表型的量与单核细胞表型的参考值进行比较，其中与参考值相比，从临床样品确定的单核细胞表型的量的差异包括 存在前肿瘤免疫反应。

公开(公告)号：US20050079174A1

公开(公告)日：2005-04-14

申请号：US10626213

申请日：2003-07-24

Applicant: Emilio Barbera-Guillem ,  M. Nelson

Inventor： Emilio Barbera-Guillem ,  M. Nelson

IPC: A61K39/395 ,  A61P25/28 ,  C07K16/28 ,  G01N33/569

CPC classification number: G01N33/56972 ,  A61K39/39541 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/2896 ,  G01N2333/70596 ,  A61K2300/00

Abstract: Methods are provided for reducing a pro-multiple sclerosis immune response by administering to an individual a composition comprising an affinity ligand which binds to B cell determinant, and which is administered in an amount effective to reduce B cells.

Abstract translation: 提供了通过向个体施用包含与B细胞决定簇结合的亲和配体的组合物并且以有效降低B细胞的量给药的方法来减少多发性硬化症免疫应答的方法。

公开(公告)号：US06821772B2

公开(公告)日：2004-11-23

申请号：US10183132

申请日：2002-06-25

Applicant: Emilio Barbera-Guillem ,  M. Bud Nelson

Inventor： Emilio Barbera-Guillem ,  M. Bud Nelson

IPC: C12M112

CPC classification number: C12N13/00 ,  C12M23/10 ,  C12M23/22 ,  C12M23/24 ,  C12M23/58 ,  C12M25/02 ,  C12M47/04 ,  C12N15/87

Abstract: Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; at least one gas permeable membrane securedly sealed in a leak-proof sealing to a frame and an opposing surface comprising a rigid surface in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.

Abstract translation: 提供培养细胞的细胞培养装置，任选地，用于进行期望培养的细胞的磁分离。 细胞培养装置优选包括框架; 将至少一个透气膜固定地密封在框架的防漏密封件中，以及包括在其间形成培养室的刚性表面的相对表面; 和至少一个可重新密封的孔，以允许物质被引入或从培养室中取出。

公开(公告)号：US06761877B2

公开(公告)日：2004-07-13

申请号：US09783469

申请日：2001-02-12

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61B1000

CPC classification number: C12Q1/6816 ,  B82Y15/00 ,  C12Q1/6818 ,  G01N33/586 ,  G01N33/588 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  C12Q2563/161 ,  C12Q2563/107 ,  C12Q2523/107

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

Abstract translation: 提供了一种官能化包封的荧光纳米晶体，其包含其中封装有一种或多种荧光纳米晶体的脂质体; 在检测系统中使用官能化封装的荧光纳米晶体; 以及制备官能化包封的荧光纳米晶体的方法。 使用具有结合到其上的亲和分子的官能化包封的荧光纳米晶体的方法包括使官能化的包封的荧光纳米晶体与样品接触，使得在官能化的包封的荧光纳米晶体和亲本分子具有结合特异性的底物之间形成复合物，如果底物是 当下; 将检测系统中的复合物暴露于激发光源，并检测从复合物发射的荧光峰（如果存在）。

公开(公告)号：US06749750B2

公开(公告)日：2004-06-15

申请号：US09975889

申请日：2001-10-11

Applicant: Emilio Barbera-Guillem ,  Marlin O. Thurston

Inventor： Emilio Barbera-Guillem ,  Marlin O. Thurston

IPC: B01D3506

CPC classification number: C12M47/04 ,  B03C1/288 ,  B32B7/12 ,  C12M23/10 ,  H01F7/0231 ,  Y10S428/90 ,  Y10T428/14 ,  Y10T428/2848 ,  Y10T428/32

Abstract: A magnetic sheet assembly for application to a device adapted for magnetic separation, the magnetic sheet assembly comprising: a magnetic sheet; a permanent, pressure sensitive adhesive; a carrier sheet; and a non-permanent pressure sensitive adhesive. Also provided is a method of mounting and removably adhering the magnetic sheet assembly to a surface of a device for magnetic separation, the method comprising the steps of aligning the magnetic sheet assembly with the surface of the device; and pressing the aligned magnetic sheet assembly in placing its non-permanent adhesive face into adhesive contact with the surface of the device.

Abstract translation: 一种用于应用于适于磁分离的装置的磁片组件，所述磁片组件包括：磁片; 永久性压敏粘合剂; 载体片; 和非永久性压敏粘合剂。 还提供了一种将磁性片组件安装和可移除地粘附到用于磁选的装置的表面的方法，该方法包括以下步骤：将磁片组件与装置的表面对准; 并且将所述对准的磁性片组件压入其非永久性粘合剂表面与所述装置的表面粘合接触。

公开(公告)号：US06680211B2

公开(公告)日：2004-01-20

申请号：US10367063

申请日：2003-02-14

Applicant: Emilio Barbera-Guillem ,  Stephanie L. Castro

Inventor： Emilio Barbera-Guillem ,  Stephanie L. Castro

IPC: G01N33546

CPC classification number: G07F7/086 ,  A61K9/167 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/588 ,  G06K19/06 ,  G07D7/1205 ,  Y10S428/916 ,  Y10T428/24802 ,  Y10T428/24893 ,  Y10T428/24901 ,  Y10T428/25 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2998

Abstract: Provided are a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals embedded in a polymeric microsphere; a kit prepared from the fluorescent microspheres; and a method of producing the fluorescent microspheres comprising swelling the polymeric microsphere so that into its pores can enter fluorescent nanocrystals, and then unswelling the polymeric micropsheres so that the fluorescent nanocrystals become physically entrapped in the pores of the unswelled polymeric microsphere. Also provided is a method of using the fluorescent microspheres comprising affinity ligand for determining the presence or absence of a predetermined number of analytes in a sample by contacting the sample with the fluorescent microspheres, and detecting the fluorescence signal pattern of excited fluorescent microspheres bound to one or more analytes of the predetermined number of analytes, if present in the sample.

Abstract translation: 提供一种荧光微球，其由嵌入聚合物微球中的多个荧光纳米晶体组成; 由荧光微球制备的试剂盒; 以及制备荧光微球的方法，其包括使聚合物微球溶胀，使得其进入其孔可以进入荧光纳米晶体，然后使聚合物微球不通溶，使得荧光纳米晶体物理地截留在未密封的聚合物微球的孔中。 还提供了使用包含亲和配体的荧光微球的方法，用于通过使样品与荧光微球接触来检测样品中是否存在预定数量的分析物，并且检测结合到一个的激发的荧光微球的荧光信号图案 或更多分析物，如果存在于样品中。

公开(公告)号：US06548171B1

公开(公告)日：2003-04-15

申请号：US09633953

申请日：2000-08-08

Applicant: Emilio Barbera-Guillem ,  Stephanie L. Castro

Inventor： Emilio Barbera-Guillem ,  Stephanie L. Castro

IPC: B32B1502

CPC classification number: G07F7/086 ,  A61K9/167 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/588 ,  G06K19/06 ,  G07D7/1205 ,  Y10S428/916 ,  Y10T428/24802 ,  Y10T428/24893 ,  Y10T428/24901 ,  Y10T428/25 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2998

Abstract: Provided are a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals embedded in a polymeric microsphere; a kit prepared from the fluorescent microspheres; and a method of producing the fluorescent microspheres comprising swelling the polymeric microsphere so that into its pores can enter fluorescent nanocrystals, and then unswelling the polymeric micropsheres so that the fluorescent nanocrystals become physically entrapped in the pores of the unswelled polymeric microsphere. Also provided is a method of using the fluorescent microspheres comprising affinity ligand for determining the presence or absence of a predetermined number of analytes in a sample by contacting the sample with the fluorescent microspheres, and detecting the fluorescence signal pattern of excited fluorescent microspheres bound to one or more analytes of the predetermined number of analytes, if present in the sample.

Abstract translation: 提供一种荧光微球，其由嵌入聚合物微球中的多个荧光纳米晶体组成; 由荧光微球制备的试剂盒; 以及制备荧光微球的方法，其包括使聚合物微球溶胀，使得其进入其孔可以进入荧光纳米晶体，然后使聚合物微球不通溶，使得荧光纳米晶体物理地截留在未密封的聚合物微球的孔中。 还提供了使用包含亲和配体的荧光微球的方法，用于通过使样品与荧光微球接触来检测样品中是否存在预定数量的分析物，并且检测结合到一个的激发的荧光微球的荧光信号图案 或更多分析物，如果存在于样品中。