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183 条记录 (耗时 0.051 秒)

    Variants of glycoside hydrolases
    41.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US08383385B2

    公开(公告)日：2013-02-26

    申请号：US13009524

    申请日：2011-01-19

    申请人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C12S3/00 ,  C12S3/02 ,  C12P19/14 ,  C11D3/386

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Method for stonewashing fabrics using cellulase
    45.
    发明授权
    Method for stonewashing fabrics using cellulase 有权
    使用纤维素酶染色织物的方法

    公开(公告)号：US08198068B2

    公开(公告)日：2012-06-12

    申请号：US12811533

    申请日：2009-02-19

    申请人： Guifang Wu ,  Sarah Teter ,  Paul Harris ,  Weijian Lai

    发明人： Guifang Wu ,  Sarah Teter ,  Paul Harris ,  Weijian Lai

    IPC分类号： D06M16/00 ,  C09B67/00 ,  C12N9/62

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  D06B11/0096 ,  D06P5/15 ,  D06P5/158

    摘要： A method of forming localized variation of color density in the surface of a dyed cellulosic fabric with reducing back staining, with a composition comprising a cellulose having the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having at least 75% sequence identity with SEQ ID NO: 2 is provided. A method for biopolishing a cellulose-containing fabric by using the new endoglucanase is also provided.

    摘要翻译： 一种在具有减少反向染色的染色纤维素织物的表面中形成颜色密度的局部变化的方法，其中组合物包含具有SEQ ID NO：2的氨基酸序列的纤维素或具有至少75％序列的氨基酸序列 提供了与SEQ ID NO：2的同一性。 还提供了通过使用新的内切葡聚糖酶来生物抛光含纤维素的织物的方法。

    Methods for increasing homologous recombination of a nucleic acid sequence
    46.
    发明授权
    Methods for increasing homologous recombination of a nucleic acid sequence 有权
    增加核酸序列同源重组的方法

    公开(公告)号：US08148155B2

    公开(公告)日：2012-04-03

    申请号：US10420035

    申请日：2003-04-21

    申请人： Paul Harris ,  Howard Brody

    发明人： Paul Harris ,  Howard Brody

    IPC分类号： C12N15/00 ,  C12N1/15 ,  C12N15/64 ,  C12N15/80

    CPC分类号： C12P21/00 ,  C12N15/80 ,  C12N15/902

    摘要： The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.

    摘要翻译： 本发明涉及用于增加引入宿主细胞的核酸序列的同源重组的方法，其包括：（a）向丝状真菌宿主细胞群体中引入编码重组蛋白和第二核酸序列的第一核酸序列 包括与丝状真菌宿主细胞的基因组同源的一个或多个区域，其中（i）重组蛋白促进一个或多个区域与宿主基因组中的相应同源区域的重组，以掺入第二核酸序列 通过同源重组，和（ii）与没有第一核酸序列的相同群体相比，包含所述群体中掺入的第二核酸序列的宿主细胞的数量增加至少20％; （b）并从群体中分离包含掺入的第二核酸序列的丝状真菌细胞。

    Polypeptides having xylanase activity from Aspergillus fumigatus
    49.
    发明授权
    Polypeptides having xylanase activity from Aspergillus fumigatus 有权
    具有来自烟曲霉的木聚糖酶活性的多肽

    公开(公告)号：US07960160B2

    公开(公告)日：2011-06-14

    申请号：US11054191

    申请日：2005-02-09

    申请人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

    发明人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

    IPC分类号： C12N9/24 ,  C12N15/56

    CPC分类号： C12N9/2482 ,  C12N9/248 ,  C12Y302/01008

    摘要： The present invention relates to an isolated polypeptide having xylanase activity from Aspergillus fumigatus with a molecular weight of approximately 50 kDa by SDS-PAGE and homologous polypeptides thereof comprising an amino acid sequence having at least 95% identity with amino adds 20 to 397 of SEQ ID NO: 6; encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with nucleotides 107 to 1415 of SEQ ID NO: 5: or encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 107 to 1415 of SEQ ID NO: 5, (ii) the cDNA sequence of (i), or (iii) a full-length complementary strand of (i) or (ii). The present invention also relates to a method for producing such polypeptides having xylanase activity comprising: (a) cultivating a wild-type Aspergillus fumigatus cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

    摘要翻译： 本发明涉及通过SDS-PAGE分析重量为约50kDa的烟曲霉具有木聚糖酶活性的分离多肽，并且其同源多肽包含与氨基具有至少95％同一性的氨基酸序列，添加SEQ ID NO：20至397 NO：6; 由包含与SEQ ID NO：5的核苷酸107至1415具有至少95％同一性的核苷酸序列的多核苷酸编码，或由在高严格条件下与（i）SEQ ID NO：5的核苷酸107至1415杂交的多核苷酸编码。 5，（ii）（i）或（iii）（i）或（ii）的全长互补链的cDNA序列。 本发明还涉及生产具有木聚糖酶活性的多肽的方法，其包括：（a）在有利于产生多肽的条件下培养野生型烟曲霉细胞; 和（b）回收多肽。