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公开(公告)号：US09090711B2
公开(公告)日：2015-07-28
申请号：US13301484
申请日：2011-11-21
申请人： Nozomu Matsukawa
发明人： Nozomu Matsukawa
摘要： A method of selectively arraying ferritin and inorganic particles on a silicon oxide substrate having a chromium, niobium or tungsten portion. An aspect of the method includes steps of: preparing a solution which contains ferritin modified at an N-terminal part of a subunit with a peptide set out in SEQ ID NO: 1, and a nonionic surfactant; and a binding step of bringing the solution in contact with the silicon oxide substrate to selectively array peptide-modified ferritin to the chromium, niobium or, tungsten portion. Another aspect of the method includes selectively arraying ferritin modified with the peptide set out in SEQ ID NO: 1, and the inorganic particles contained in ferritin at the chromium, niobium, or tungsten portion by removing the solution.
摘要翻译： 在具有铬，铌或钨部分的氧化硅衬底上选择性排列铁蛋白和无机颗粒的方法。 该方法的一个方面包括以下步骤：制备含有在亚基的N-末端部分修饰的铁蛋白与SEQ ID NO：1所示肽的溶液和非离子表面活性剂; 以及使溶液与氧化硅衬底接触以选择性地将肽修饰的铁蛋白排列到铬，铌或钨部分的结合步骤。 该方法的另一方面包括通过除去溶液来选择性地排列用SEQ ID NO：1所示的肽修饰的铁蛋白和包含在铬，铌或钨部分的铁蛋白中的无机颗粒。
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公开(公告)号：US20120309033A1
公开(公告)日：2012-12-06
申请号：US13536202
申请日：2012-06-28
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
IPC分类号： G01N21/78
CPC分类号： G01N33/542 , C12Q1/26 , G01N33/54373 , G01N2333/90287
摘要： The present invention relates to a method for detecting an antigen with use of an antibody and an enzyme. Specifically, the present invention provides a method for detecting an antigen without use of a labeled-antibody. the method comprises immersing particles in a first buffer solution which is predicted to contain the antigen; wherein an antibody and a multi-copper oxidase CueO are immobilized on each surface of the particles, and the antibody reacts specifically with the antigen. The method further comprises the following steps recovering the obtained particles; mixing the particles recovered, an oxidation-reduction indicator (reductant), and a second buffer solution so as to prepare a reaction solution; measuring an activity degree of the multi-copper oxidase CueO contained in the reaction solution; determining that the first buffer solution contains the antigen based on the above activity degree.
摘要翻译： 本发明涉及使用抗体和酶检测抗原的方法。 具体而言，本发明提供了不使用标记抗体来检测抗原的方法。 该方法包括将颗粒浸入预测含有抗原的第一缓冲溶液中; 其中抗体和多铜氧化酶CueO固定在颗粒的每个表面上，并且抗体与抗原特异性反应。 该方法还包括以下步骤：回收所获得的颗粒; 混合回收的颗粒，氧化还原指示剂（还原剂）和第二缓冲溶液以制备反应溶液; 测定反应溶液中所含的多铜氧化酶CueO的活度; 基于上述活性度确定第一缓冲溶液含有抗原。
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公开(公告)号：US08309345B2
公开(公告)日：2012-11-13
申请号：US13296879
申请日：2011-11-15
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
CPC分类号： G01N33/54306 , C12Q1/26
摘要： Provided is a method for detecting an antigen without use of a labeled-antibody. A support having an antibody and a multi-copper oxidase CueO immobilized thereon is brought into contact with a first buffer solution containing the antigen, a current is measured by a potentiostat method using the support and a second buffer solution, and when the measured current is greater than or equal to 1.5×(blank value), it is determined that the antigen exists. The second buffer solution contains a substrate of the CueO and has an ionic strength falling within a range of not less than 0.3 mM and not more than 1.0 mM.
摘要翻译： 提供了不使用标记抗体来检测抗原的方法。 使具有固定在其上的抗体和多铜氧化酶CueO的载体与含有抗原的第一缓冲溶液接触，通过使用载体和第二缓冲溶液的恒电位法测量电流，并且当测量的电流为 大于或等于1.5×（空白值），则确定存在抗原。 第二缓冲溶液含有CueO的底物，离子强度在0.3mM以上1.0mM以下的范围内。
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公开(公告)号：US20090203552A1
公开(公告)日：2009-08-13
申请号：US12270336
申请日：2008-11-13
申请人： Takuro MATSUI , Nozomu Matsukawa , Kazuaki Nishio
发明人： Takuro MATSUI , Nozomu Matsukawa , Kazuaki Nishio
IPC分类号： C40B50/14
摘要： A novel method for two-dimensionally arraying ferritin on a substrate is provided which obviates the need for a metal ion that permits linking between adjacent two ferritin particles. In a method of two-dimensionally arraying ferritin on a substrate, the surface of the substrate is hydrophilic, and the method includes the steps of: developing a solution containing a solvent and the ferritin on the substrate; and removing the solvent from the solution developed on the substrate, while the ferritin has an amino acid sequence set out in SEQ ID NO: 1 modified at its N-terminus.
摘要翻译： 提供了一种用于在基底上二维排列铁蛋白的新方法，其消除了允许在相邻的两个铁蛋白颗粒之间连接的金属离子的需要。 在基板上二维排列铁蛋白的方法中，基板的表面是亲水性的，该方法包括以下步骤：在基板上显影含有溶剂和铁蛋白的溶液; 并从底物上显影的溶液中除去溶剂，而铁蛋白具有在其N末端修饰的SEQ ID NO：1所示的氨基酸序列。
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公开(公告)号：US09090710B2
公开(公告)日：2015-07-28
申请号：US13299737
申请日：2011-11-18
申请人： Nozomu Matsukawa
发明人： Nozomu Matsukawa
摘要： A method of selectively arraying ferritin and inorganic particles on a silicon oxide substrate at regions having vanadium, niobium or tantalum. An aspect of the method includes steps of: preparing a solution which contains ferritin modified at an N-terminal part of a subunit with a peptide set out in SEQ ID NO: 1, and from 0.01 v/v % to 10 v/v % of a nonionic surfactant and having a pH of from 7.4 to 8.2; and a binding step of bringing the solution in contact with regions of the substrate having vanadium, niobium, or tantalum to selectively array peptide-modified ferritin to vanadium, niobium or, tantalum portion. The method may also include a step of selectively arraying ferritin modified with the peptide set out in SEQ ID NO: 1, and the inorganic particles contained in ferritin at the vanadium, niobium, or tantalum portion by removing the solution.
摘要翻译： 在具有钒，铌或钽的区域，在氧化硅衬底上选择性排列铁蛋白和无机颗粒的方法。 该方法的一个方面包括以下步骤：制备含有在亚基的N末端部分修饰的铁蛋白与SEQ ID NO：1所示肽和0.01v / v％至10v / v％ 的非离子表面活性剂，pH为7.4至8.2; 以及使溶液与具有钒，铌或钽的基板的区域接触以选择性地将肽修饰的铁蛋白排列到钒，铌或钽部分的结合步骤。 该方法还可以包括通过除去溶液来选择性地排列用SEQ ID NO：1所示的肽修饰的铁蛋白和包含在钒，铌或钽部分的铁蛋白中的无机颗粒的步骤。
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公开(公告)号：US08470549B2
公开(公告)日：2013-06-25
申请号：US13536202
申请日：2012-06-28
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
CPC分类号： G01N33/542 , C12Q1/26 , G01N33/54373 , G01N2333/90287
摘要： The present invention relates to a method for detecting an antigen with use of an antibody and an enzyme. Specifically, the present invention provides a method for detecting an antigen without use of a labeled-antibody. the method comprises immersing particles in a first buffer solution which is predicted to contain the antigen; wherein an antibody and a multi-copper oxidase CueO are immobilized on each surface of the particles, and the antibody reacts specifically with the antigen. The method further comprises the following steps recovering the obtained particles; mixing the particles recovered, an oxidation-reduction indicator (reductant), and a second buffer solution so as to prepare a reaction solution; measuring an activity degree of the multi-copper oxidase CueO contained in the reaction solution; determining that the first buffer solution contains the antigen based on the above activity degree.
摘要翻译： 本发明涉及使用抗体和酶检测抗原的方法。 具体而言，本发明提供了不使用标记抗体来检测抗原的方法。 该方法包括将颗粒浸入预测含有抗原的第一缓冲溶液中; 其中抗体和多铜氧化酶CueO固定在颗粒的每个表面上，并且抗体与抗原特异性反应。 该方法还包括以下步骤：回收所获得的颗粒; 混合回收的颗粒，氧化还原指示剂（还原剂）和第二缓冲溶液以制备反应溶液; 测定反应溶液中所含的多铜氧化酶CueO的活度; 基于上述活性度确定第一缓冲溶液含有抗原。
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公开(公告)号：US20080153713A1
公开(公告)日：2008-06-26
申请号：US11952632
申请日：2007-12-07
申请人： Takuro MATSUI , Nozomu Matsukawa
发明人： Takuro MATSUI , Nozomu Matsukawa
IPC分类号： C40B50/18
摘要： The present invention provides a novel method of two-dimensionally arraying ferritin on a substrate, which obviates the need for a metal ion for achieving linking between two adjacent ferritin. The present invention provides a method of two-dimensionally arraying ferritin on a substrate, wherein the ferritin has an amino acid sequence set out in SEQ ID NO: 1 on the outer peripheral surface; the surface of the substrate is hydrophilic; and the method includes a development step of developing a solution that contains a solvent, the ferritin, and 6.5 mM to 52 mM ammonium sulfate on the substrate, and a removal step of removing the solvent from the solution developed on the substrate.
摘要翻译： 本发明提供了一种在基板上二维排列铁蛋白的新方法，其不需要用于实现两个相邻铁蛋白之间的连接的金属离子。 本发明提供了在基板上二维排列铁蛋白的方法，其中，铁蛋白在外周面上具有SEQ ID NO：1所示的氨基酸序列; 基材的表面是亲水性的; 并且该方法包括显影步骤，其在基底上开发含有溶剂，铁蛋白和6.5mM至52mM硫酸铵的溶液，以及从在基底上显影的溶液中除去溶剂的去除步骤。
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48.发明申请公开(公告)号：US20060070494A1
公开(公告)日：2006-04-06
申请号：US11284910
申请日：2005-11-23
IPC分类号： B22F9/20
CPC分类号： B82Y10/00 , H01L21/28273 , H01L21/28282 , H01L29/42332
摘要： An object of the present invention is to provide a method of forming fine particles on a substrate in which reoxidization of reduced fine particles is suppressed. Reduced fine particles (FeO fine particles) are formed by embedding metal oxide fine particles (Fe2O3 fine particles) fixed on a p type silicon semiconductor substrate into a silicon oxidized film, and carrying out a heat treatment in a reducing gas atmosphere. Presence of the silicon oxidized film enables suppression of reoxidization of the reduced fine particles (FeO fine particles) due to exposure to the ambient air.
摘要翻译： 本发明的目的是提供一种在抑制还原的微粒的再氧化的基板上形成微粒的方法。 通过将固定在p型硅半导体衬底上的金属氧化物微粒（Fe 2 O 3 O 3微粒）嵌入到硅氧化膜中而形成还原的微粒（FeO微粒） ，并在还原气体气氛中进行热处理。 硅氧化膜的存在能够抑制由于暴露于环境空气而导致的还原的微粒（FeO微粒）的再氧化。
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49.发明授权公开(公告)号：US07419529B2
公开(公告)日：2008-09-02
申请号：US11284910
申请日：2005-11-23
IPC分类号： B22F9/20
CPC分类号： B82Y10/00 , H01L21/28273 , H01L21/28282 , H01L29/42332
摘要： An object of the present invention is to provide a method of forming fine particles on a substrate in which reoxidization of reduced fine particles is suppressed. Reduced fine particles (FeO fine particles) are formed by embedding metal oxide fine particles (Fe2O3 fine particles) fixed on a p type silicon semiconductor substrate into a silicon oxidized film, and carrying out a heat treatment in a reducing gas atmosphere. Presence of the silicon oxidized film enables suppression of reoxidization of the reduced fine particles (FeO fine particles) due to exposure to the ambient air.
摘要翻译： 本发明的目的是提供一种在抑制还原的微粒的再氧化的基板上形成微粒的方法。 通过将固定在p型硅半导体衬底上的金属氧化物微粒（Fe 2 O 3 O 3微粒）嵌入到硅氧化膜中而形成还原的微粒（FeO微粒） ，并在还原气体气氛中进行热处理。 硅氧化膜的存在能够抑制由于暴露于环境空气而导致的还原的微粒（FeO微粒）的再氧化。
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公开(公告)号：US20080161205A1
公开(公告)日：2008-07-03
申请号：US11952609
申请日：2007-12-07
申请人： Takuro Matsui , Nozomu Matsukawa
发明人： Takuro Matsui , Nozomu Matsukawa
IPC分类号： C40B50/18
摘要： The present invention provides a novel method of two-dimensionally arraying ferritin on a substrate, which obviates the need for a metal ion for achieving linking between two adjacent ferritin. The present invention provides a method of two-dimensionally arraying ferritin on a substrate, wherein the ferritin has an amino acid sequence set out in SEQ ID NO: 1 on the outer peripheral surface; the surface of the substrate is hydrophilic; and the method includes a development step of developing a solution that contains a solvent, the ferritin, and 2 mM to 100 mM ammonium acetate on the substrate, and a removal step of removing the solvent from the solution developed on the substrate.
摘要翻译： 本发明提供了一种在基板上二维排列铁蛋白的新方法，其不需要用于实现两个相邻铁蛋白之间的连接的金属离子。 本发明提供了在基板上二维排列铁蛋白的方法，其中，铁蛋白在外周面上具有SEQ ID NO：1所示的氨基酸序列; 基材的表面是亲水性的; 并且该方法包括显影步骤，其在基底上显影含有溶剂，铁蛋白和2mM至100mM乙酸铵的溶液，以及从在基底上显影的溶液中除去溶剂的去除步骤。
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