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公开(公告)号：US08470549B2
公开(公告)日：2013-06-25
申请号：US13536202
申请日：2012-06-28
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
CPC分类号： G01N33/542 , C12Q1/26 , G01N33/54373 , G01N2333/90287
摘要： The present invention relates to a method for detecting an antigen with use of an antibody and an enzyme. Specifically, the present invention provides a method for detecting an antigen without use of a labeled-antibody. the method comprises immersing particles in a first buffer solution which is predicted to contain the antigen; wherein an antibody and a multi-copper oxidase CueO are immobilized on each surface of the particles, and the antibody reacts specifically with the antigen. The method further comprises the following steps recovering the obtained particles; mixing the particles recovered, an oxidation-reduction indicator (reductant), and a second buffer solution so as to prepare a reaction solution; measuring an activity degree of the multi-copper oxidase CueO contained in the reaction solution; determining that the first buffer solution contains the antigen based on the above activity degree.
摘要翻译： 本发明涉及使用抗体和酶检测抗原的方法。 具体而言，本发明提供了不使用标记抗体来检测抗原的方法。 该方法包括将颗粒浸入预测含有抗原的第一缓冲溶液中; 其中抗体和多铜氧化酶CueO固定在颗粒的每个表面上，并且抗体与抗原特异性反应。 该方法还包括以下步骤：回收所获得的颗粒; 混合回收的颗粒，氧化还原指示剂（还原剂）和第二缓冲溶液以制备反应溶液; 测定反应溶液中所含的多铜氧化酶CueO的活度; 基于上述活性度确定第一缓冲溶液含有抗原。
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公开(公告)号：US20120309033A1
公开(公告)日：2012-12-06
申请号：US13536202
申请日：2012-06-28
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
IPC分类号： G01N21/78
CPC分类号： G01N33/542 , C12Q1/26 , G01N33/54373 , G01N2333/90287
摘要： The present invention relates to a method for detecting an antigen with use of an antibody and an enzyme. Specifically, the present invention provides a method for detecting an antigen without use of a labeled-antibody. the method comprises immersing particles in a first buffer solution which is predicted to contain the antigen; wherein an antibody and a multi-copper oxidase CueO are immobilized on each surface of the particles, and the antibody reacts specifically with the antigen. The method further comprises the following steps recovering the obtained particles; mixing the particles recovered, an oxidation-reduction indicator (reductant), and a second buffer solution so as to prepare a reaction solution; measuring an activity degree of the multi-copper oxidase CueO contained in the reaction solution; determining that the first buffer solution contains the antigen based on the above activity degree.
摘要翻译： 本发明涉及使用抗体和酶检测抗原的方法。 具体而言，本发明提供了不使用标记抗体来检测抗原的方法。 该方法包括将颗粒浸入预测含有抗原的第一缓冲溶液中; 其中抗体和多铜氧化酶CueO固定在颗粒的每个表面上，并且抗体与抗原特异性反应。 该方法还包括以下步骤：回收所获得的颗粒; 混合回收的颗粒，氧化还原指示剂（还原剂）和第二缓冲溶液以制备反应溶液; 测定反应溶液中所含的多铜氧化酶CueO的活度; 基于上述活性度确定第一缓冲溶液含有抗原。
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公开(公告)号：US08309345B2
公开(公告)日：2012-11-13
申请号：US13296879
申请日：2011-11-15
申请人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
发明人： Kazuaki Nishio , Nozomu Matsukawa , Shigeo Yoshii
CPC分类号： G01N33/54306 , C12Q1/26
摘要： Provided is a method for detecting an antigen without use of a labeled-antibody. A support having an antibody and a multi-copper oxidase CueO immobilized thereon is brought into contact with a first buffer solution containing the antigen, a current is measured by a potentiostat method using the support and a second buffer solution, and when the measured current is greater than or equal to 1.5×(blank value), it is determined that the antigen exists. The second buffer solution contains a substrate of the CueO and has an ionic strength falling within a range of not less than 0.3 mM and not more than 1.0 mM.
摘要翻译： 提供了不使用标记抗体来检测抗原的方法。 使具有固定在其上的抗体和多铜氧化酶CueO的载体与含有抗原的第一缓冲溶液接触，通过使用载体和第二缓冲溶液的恒电位法测量电流，并且当测量的电流为 大于或等于1.5×（空白值），则确定存在抗原。 第二缓冲溶液含有CueO的底物，离子强度在0.3mM以上1.0mM以下的范围内。
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公开(公告)号：US20090203552A1
公开(公告)日：2009-08-13
申请号：US12270336
申请日：2008-11-13
申请人： Takuro MATSUI , Nozomu Matsukawa , Kazuaki Nishio
发明人： Takuro MATSUI , Nozomu Matsukawa , Kazuaki Nishio
IPC分类号： C40B50/14
摘要： A novel method for two-dimensionally arraying ferritin on a substrate is provided which obviates the need for a metal ion that permits linking between adjacent two ferritin particles. In a method of two-dimensionally arraying ferritin on a substrate, the surface of the substrate is hydrophilic, and the method includes the steps of: developing a solution containing a solvent and the ferritin on the substrate; and removing the solvent from the solution developed on the substrate, while the ferritin has an amino acid sequence set out in SEQ ID NO: 1 modified at its N-terminus.
摘要翻译： 提供了一种用于在基底上二维排列铁蛋白的新方法，其消除了允许在相邻的两个铁蛋白颗粒之间连接的金属离子的需要。 在基板上二维排列铁蛋白的方法中，基板的表面是亲水性的，该方法包括以下步骤：在基板上显影含有溶剂和铁蛋白的溶液; 并从底物上显影的溶液中除去溶剂，而铁蛋白具有在其N末端修饰的SEQ ID NO：1所示的氨基酸序列。
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公开(公告)号：US07825070B2
公开(公告)日：2010-11-02
申请号：US12270336
申请日：2008-11-13
申请人： Takuro Matsui , Nozomu Matsukawa , Kazuaki Nishio
发明人： Takuro Matsui , Nozomu Matsukawa , Kazuaki Nishio
摘要： A novel method for two-dimensionally arraying ferritin on a substrate is provided which obviates the need for a metal ion that permits linking between adjacent two ferritin particles. In a method of two-dimensionally arraying ferritin on a substrate, the surface of the substrate is hydrophilic, and the method includes the steps of: developing a solution containing a solvent and the ferritin on the substrate; and removing the solvent from the solution developed on the substrate, while the ferritin has an amino acid sequence set out in SEQ ID NO: 1 modified at its N-terminus.
摘要翻译： 提供了一种用于在基底上二维排列铁蛋白的新方法，其消除了允许在相邻的两个铁蛋白颗粒之间连接的金属离子的需要。 在基板上二维排列铁蛋白的方法中，基板的表面是亲水性的，该方法包括以下步骤：在基板上显影含有溶剂和铁蛋白的溶液; 并从底物上显影的溶液中除去溶剂，而铁蛋白具有在其N末端修饰的SEQ ID NO：1所示的氨基酸序列。
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6.发明授权公开(公告)号：US07919596B2
公开(公告)日：2011-04-05
申请号：US12320556
申请日：2009-01-29
IPC分类号： C07K14/00 , H01L21/00 , H01L21/314 , H01L21/471
摘要： To provide a method of arranging ferritin by which a high rate of the number of the molecular film spots on which sole ferritin molecule was arranged in effect, with respect to total number of the molecular film spots provided for arranging ferritin (sole arrangement rate) is achieved is objected to. Specifically, in Fer8 ferritin having a sequence excluding 7 amino acids of from the second to the eighth, from an amino acid sequence (Fer0 sequence) translated from a naturally occurring DNA sequence, lysine at position 91 is substituted with glutamic acid.
摘要翻译： 为了提供一种安排铁蛋白的方法，相对于提供用于安排铁蛋白的总分子膜点数（单独排列速率），通过该铁蛋白排列高分子膜斑点的数量，单个铁蛋白分子排列有效的方法是： 实现是反对的。 具体而言，在来自第二至第八的7个氨基酸序列的Fer8铁蛋白中，从天然存在的DNA序列翻译的氨基酸序列（Fer0序列）中，91位的赖氨酸被谷氨酸取代。
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7.发明申请公开(公告)号：US20090187010A1
公开(公告)日：2009-07-23
申请号：US12320556
申请日：2009-01-29
IPC分类号： C07K17/00
摘要： To provide a method of arranging ferritin by which a high rate of the number of the molecular film spots on which sole ferritin molecule was arranged in effect, with respect to total number of the molecular film spots provided for arranging ferritin (sole arrangement rate) is achieved is objected to. Specifically, in Fer8 ferritin having a sequence excluding 7 amino acids of from the second to the eighth, from an amino acid sequence (Fer0 sequence) translated from a naturally occurring DNA sequence, lysine at position 91 is substituted with glutamic acid.
摘要翻译： 为了提供一种安排铁蛋白的方法，相对于提供用于安排铁蛋白的总分子膜点数（单独排列速率），通过该铁蛋白排列高分子膜斑点的数量，单个铁蛋白分子排列有效的方法是： 实现是反对的。 具体而言，在来自第二至第八的7个氨基酸序列的Fer8铁蛋白中，从天然存在的DNA序列翻译的氨基酸序列（Fer0序列）中，91位的赖氨酸被谷氨酸取代。
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8.发明授权Electrode, electrically heating type catalyst device using same, and manufacturing method of electrically heating type catalyst device 有权电极，使用该电极的电加热型催化剂装置，以及电加热型催化剂装置的制造方法公开(公告)号：US08815167B2
公开(公告)日：2014-08-26
申请号：US13577368
申请日：2011-09-14
IPC分类号： B01D50/00
CPC分类号： H05B3/08 , H05B3/42 , H05B2203/022 , H05B2203/024
摘要： An electrode according to one aspect of the present invention is formed on a base material composed of a ceramics. The electrodes includes a matrix composed of an Ni—Cr alloy (with a Cr content of 20 to 60 wt. %) or an MCrAlY alloy (M is at least one material selected from Fe, Co and Ni), and a disperse phase that is dispersed in the matrix and composed of an oxide mineral having a laminated structure. The ratio of area occupied by the disperse phase in a cross section of the electrode is 40 to 80%. With the structure like this, it is possible to suppress the increase in the electrical resistance even after a thermal cycle is performed.
摘要翻译： 根据本发明的一个方面的电极形成在由陶瓷构成的基材上。 电极包括由Ni-Cr合金（Cr含量为20〜60重量％）或MCrAlY合金（M为选自Fe，Co和Ni中的至少一种材料）构成的基体，以及分散相， 分散在基质中并由具有层压结构的氧化物矿物构成。 电极截面中分散相所占的面积比为40〜80％。 利用这样的结构，即使在进行热循环之后也可以抑制电阻的增加。
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公开(公告)号：US4696994A
公开(公告)日：1987-09-29
申请号：US809528
申请日：1985-12-16
申请人： Kohei Nakajima , Kazuaki Nishio , Hiroshi Watanabe
发明人： Kohei Nakajima , Kazuaki Nishio , Hiroshi Watanabe
CPC分类号： C08G73/105 , C08G73/1064
摘要： A transparent aromatic polyimide produced by polymerization and imidation of an aromatic tetracarboxylic acid containing as a principal component biphenyltetracarboxylic acid and an aromatic diamine containing as a principal component bis(aminophenoxy-phenyl)sulfone of the formula (I) or bis(aminophenoxy-phenyl)propane of the formula (II): ##STR1##
摘要翻译： 以通式（I）的双（氨基苯氧基 - 苯基）砜或双（氨基苯氧基 - 苯基）苯酚为主要成分，以二苯基四羧酸为主要成分的芳香族四羧酸的聚合和酰亚胺化产生的透明芳香族聚酰亚胺， （II）的丙烷：（Ⅰ）（Ⅰ）
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公开(公告)号：US4673773A
公开(公告)日：1987-06-16
申请号：US705745
申请日：1985-02-26
申请人： Tsunetomo Nakano , Hiroshi Yasuno , Kazuaki Nishio
发明人： Tsunetomo Nakano , Hiroshi Yasuno , Kazuaki Nishio
IPC分类号： C08G69/32 , C08G69/00 , C08G69/26 , C08G69/48 , G03F7/038 , H05K1/00 , H05K3/00 , H05K3/18 , H05K3/46 , H05K1/14
CPC分类号： G03F7/0387 , C08G69/265 , H05K3/4676 , H05K2201/09563 , H05K3/0023 , H05K3/181
摘要： In a multilayer printed circuit board comprising an insulating substrate and a plurality of circuit elements provided thereon in layers, each of which is composed of a circuit pattern and an insulating resin layer provided over the circuit pattern, the circuit patterns arranged adjacently to each other being portionwise connected electrically through via at least one hole, the improvement in which said insulating resin layer comprises a photoset resin of an aromatic polyamide having at least 10 molar % of a constitutional unit of the formula (Ia): ##STR1## wherein each of R.sup.1 and R.sup.2 independently is a hydrogen atom or a residue of a reactive organic compound, Ar.sup.1 is a divalent aromatic residue, and Ar.sup.2 is a divalent aromatic residue possessing a photosensitive group.
摘要翻译： 在包括绝缘基板和设置在其上的多个电路元件的多层印刷电路板中，每个电路板由设置在电路图案上的电路图案和绝缘树脂层构成，彼此相邻布置的电路图案是 其中所述绝缘树脂层包括具有至少10摩尔％的式（Ia）的结构单元的芳族聚酰胺的光固化树脂：其中， R 1和R 2各自独立地为氢原子或反应性有机化合物的残基，Ar 1为二价芳香族残基，Ar 2为具有感光性基团的2价芳香族残基。
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