公开(公告)号：US20090317864A1

公开(公告)日：2009-12-24

申请号：US12493860

申请日：2009-06-29

申请人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

发明人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

IPC分类号： C12P21/02 ,  C12N9/26 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21

CPC分类号： C12N9/2417 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12Y302/01001

摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

公开(公告)号：US20090142818A1

公开(公告)日：2009-06-04

申请号：US12300140

申请日：2007-05-11

申请人： Henrik Bisgard-Frantzen ,  Kevin S. Wenger ,  Michael Trent Elder ,  Randy Deinhammer ,  Joyce Aldridge Craig

发明人： Henrik Bisgard-Frantzen ,  Kevin S. Wenger ,  Michael Trent Elder ,  Randy Deinhammer ,  Joyce Aldridge Craig

IPC分类号： C12P7/06

CPC分类号： C12P7/06 ,  C12N9/2417 ,  C12N9/2428 ,  C12Y302/01001 ,  Y02E50/17

摘要： The present invention relates to a process of producing a fermentation product, especially ethanol, from starch-containing material using an alpha-amylase and a carbohydrate-source generating enzyme. The invention also relates to a composition comprising an alpha-amylase and a carbohydrate-source generating enzyme as well as the use such compositions for producing fermentation products.

摘要翻译： 本发明涉及使用α-淀粉酶和碳水化合物源产生酶从含淀粉材料生产发酵产物，特别是乙醇的方法。 本发明还涉及包含α-淀粉酶和碳水化合物源产生酶的组合物，以及用于生产发酵产物的组合物。

公开(公告)号：US20080227174A1

公开(公告)日：2008-09-18

申请号：US12115123

申请日：2008-05-05

申请人： Henrik Bisgard-Frantzen ,  Allan Svendsen

发明人： Henrik Bisgard-Frantzen ,  Allan Svendsen

IPC分类号： C12N9/44

CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12N9/246 ,  C12P19/16 ,  C12Y302/01041 ,  C12Y302/01068

摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

摘要翻译： 本发明涉及母体淀粉脱支酶的遗传工程改造，即支链淀粉酶或淀粉酶，所述酶变体在与亲本酶相比在4-6的范围内具有改善的热稳定性和/或增加的活性 对于支链淀粉和/或糖原，与制备具有改进的热稳定性和/或改变的底物特异性的淀粉脱支酶变体的方法以及使用至少一种这样的酶变体将淀粉转化为一种或多种糖的方法 。

公开(公告)号：US20080227166A1

公开(公告)日：2008-09-18

申请号：US10586097

申请日：2005-01-14

申请人： Eric Allain ,  Kevin S. Wenger ,  Henrik Bisgard-Frantzen ,  Lori Henderson ,  Carmen Costable

发明人： Eric Allain ,  Kevin S. Wenger ,  Henrik Bisgard-Frantzen ,  Lori Henderson ,  Carmen Costable

IPC分类号： C12P7/06

CPC分类号： Y02E50/17

摘要： The present invention provides an improved process for producing a fermentation product in a fermentation medium, including ethanol, which process include a fermentation step, comprising subjecting the fermentation medium to at least one surfactant and at least one carbohydrate-source generating enzyme.

摘要翻译： 本发明提供了在发酵培养基（包括乙醇）中生产发酵产物的改进方法，该方法包括发酵步骤，包括使发酵培养基经受至少一种表面活性剂和至少一种产生碳水化合物源的酶。

公开(公告)号：US5753460A

公开(公告)日：1998-05-19

申请号：US720899

申请日：1996-10-10

申请人： Henrik Bisgard-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

发明人： Henrik Bisgard-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N9/28 ,  C12N15/56 ,  C12R1/125 ,  D06L1/14 ,  C12P21/06 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/2417 ,  C11D3/386 ,  D06L1/14

摘要： A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

摘要翻译： 与母体酶相比，具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体，其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中，条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改，母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。