公开(公告)号：US08524055B2

公开(公告)日：2013-09-03

申请号：US13094356

申请日：2011-04-26

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327 ,  G01N33/66

CPC分类号： G01N27/3272 ,  A61B5/14546 ,  A61B5/1486 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3274 ,  G01N27/3277 ,  G01N27/413 ,  G01N33/49

摘要： The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

摘要翻译： 本发明提供一种测定血液成分的方法，通过该方法能够以高精度，高可靠性测定血细胞量和干扰物质的量，并且能够根据血液的量准确地校正成分的量 细胞和干扰物质。 在用于测量血液成分的传感器中，第一工作电极13测量在血液成分的氧化还原反应期间流动的电流，第二工作电极17测量血细胞的量，第三工作电极12测量 干扰物质。 接下来，基于测定结果，校正被测量的血液成分的量。 因此，可以实现对血液成分量的更准确和精确的测量。

公开(公告)号：US08163962B2

公开(公告)日：2012-04-24

申请号：US12742873

申请日：2008-11-14

申请人： Tetsuya Ooyama ,  Takashi Onozawa ,  Shin Ikeda ,  Yoshinobu Suzuki

发明人： Tetsuya Ooyama ,  Takashi Onozawa ,  Shin Ikeda ,  Yoshinobu Suzuki

IPC分类号： C07C45/61

CPC分类号： C07C45/80 ,  C07C2602/08 ,  C07C49/67

摘要： [Problems to be Solved]The present invention aims to provide a method for efficiently producing a high-purity alkoxyindanone derivative while maintaining an industrially superior volumetric efficiency.[Solution]Provided is a method for producing an alkoxyindanone derivative represented by a general formula in FIG. 2 (wherein R represents an alkoxy group containing 1 to 6 carbon atoms and n represents an integer of 1 to 4), comprising reacting an alkoxyphenylpropionic acid derivative represented by a general formula in FIG. 1 (wherein R and n are as defined above) with a condensing agent, adding an organic solvent to the resulting reaction mixture, and subsequently decomposing the condensing agent with an aqueous alkaline solution.

摘要翻译： [待解决的问题]本发明的目的在于提供一种有效地制造高纯度烷氧基茚满酮衍生物的方法，同时保持了工业上优异的体积效率。 [解决方案]提供一种制备图1中通式所示的烷氧基茚满酮衍生物的方法。 2（其中R表示含有1至6个碳原子的烷氧基，n表示1至4的整数），包括使由图1中的通式表示的烷氧基苯基丙酸衍生物反应。 1（其中R和n如上定义）与缩合剂反应，向所得反应混合物中加入有机溶剂，随后用碱性水溶液分解缩合剂。

公开(公告)号：US07955492B2

公开(公告)日：2011-06-07

申请号：US10598001

申请日：2005-04-18

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/26 ,  G01N27/327

CPC分类号： G01N27/3272 ,  A61B5/14546 ,  A61B5/1486 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3274 ,  G01N27/3277 ,  G01N27/413 ,  G01N33/49

摘要： The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

摘要翻译： 本发明提供一种测定血液成分的方法，通过该方法能够以高精度，高可靠性测定血细胞量和干扰物质的量，并且能够根据血液的量准确地校正成分的量 细胞和干扰物质。 在用于测量血液成分的传感器中，第一工作电极13测量在血液成分的氧化还原反应期间流动的电流，第二工作电极17测量血细胞的量，第三工作电极12测量 干扰物质。 接下来，基于测定结果，校正被测量的血液成分的量。 因此，可以实现对血液成分量的更准确和精确的测量。

公开(公告)号：US06969450B2

公开(公告)日：2005-11-29

申请号：US10616305

申请日：2003-07-10

申请人： Yuko Taniike ,  Mariko Miyashita ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Yoshinobu Tokuno

发明人： Yuko Taniike ,  Mariko Miyashita ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Yoshinobu Tokuno

IPC分类号： A61B5/00 ,  G01N27/30 ,  G01N33/487 ,  C12M1/00

CPC分类号： G01N33/4875 ,  A61B5/1486 ,  A61B2562/0295

摘要： In biosensor system 1, biosensor 2 is mounted in measuring apparatus 3 for quantifying or detecting existence of a targeted substrate in a sample solution. Mounting end portion 20 of biosensor 2 to be mounted in measuring apparatus 3 has an extension of one base plate in the length direction, and further extensions of the other base plate in the width direction. Sensor mounting portion 30 of measuring apparatus 3 has a shape matching up with the shape of mounting end portion 20. Thereby wrong insertion of biosensor 2 in measuring apparatus 3 can be prevented.Further, varying shapes of plural biosensors 5 and 6 to have a common part and a non-common part, such biosensors can be handily measured by one measuring apparatus 7.

摘要翻译： 在生物传感器系统1中，将生物传感器2安装在用于定量或检测样品溶液中目标基板的存在的测量装置3中。 安装在测量装置3中的生物传感器2的安装端部20在长度方向上具有一个基板的延伸部，另一个底板在宽度方向上延伸。 测量装置3的传感器安装部30具有与安装端部20的形状相匹配的形状。 从而可以防止生物传感器2在测量装置3中错误插入。 此外，多个生物传感器5和6的不同形状具有公共部分和非共同部分，可以通过一个测量装置7轻松测量这样的生物传感器。

公开(公告)号：US20050175509A1

公开(公告)日：2005-08-11

申请号：US10515602

申请日：2003-12-19

申请人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

发明人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

IPC分类号： C12Q1/00 ,  G01N27/30

CPC分类号： C12Q1/006 ,  C12Q1/004 ,  G01N2333/904

摘要： The present invention relates to a biosensor which comprises an electrode system including at least one pair of electrodes, at least one insulating base plate for supporting the electrode system, a first reaction layer provided at least on a working electrode of the electrode system, including an organic compound having a functional group capable of bonding or being adsorbed to an electrode and a hydrophobic hydrocarbon group, a second reaction layer provided on the first reaction layer, including an amphiphilic lipid capable of bonding or being adsorbed to a hydrophobic portion of the first reaction layer, and a reagent system carried in a two-component membrane composed of the first and second reaction layers, including at least membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase and an electron mediator.

摘要翻译： 本发明涉及一种生物传感器，其包括电极系统，该电极系统包括至少一对电极，至少一个用于支撑电极系统的绝缘基板，至少设置在电极系统的工作电极上的第一反应层， 具有能够键合或吸附到电极和疏水性烃基的官能团的有机化合物，设置在第一反应层上的第二反应层，包括能够键合或吸附到第一反应的疏水部分的两亲性脂质 以及由第一反应层和第二反应层构成的双组分膜中携载的至少包含膜结合型吡喹啉醌依赖性葡萄糖脱氢酶和电子介体的试剂系统。

公开(公告)号：US08691072B2

公开(公告)日：2014-04-08

申请号：US12377514

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N27/26

CPC分类号： G01N27/3274 ,  G01N27/3272 ,  G01N27/413

摘要： Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) having branch parts (18a, 18b). A first reagent (13) consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (18b), and a second reagent (19) consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part (18a).

摘要翻译： 在氧化还原物质中的氧化剂基本上不与工作电极接触但与对电极接触的状态下，跨越对置电极和与血液样品接触的工作电极施加电压， 还原剂基本上不与对电极接触，而是与工作电极接触，由此还原剂和氧化剂分别被氧化和还原，以测量在氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的Hct值。 该测量可以用包括工作电极（11），对电极（12）和具有分支部分（18a，18b）的血液样本保持部分（14）的传感器芯片进行。 基本上由氧化还原物质中的氧化剂组成的第一试剂（13）被设置成与面对分支部分（18b）的对电极的表面接触，并且基本上由还原剂组成的第二试剂（19）设置在 与面向分支部（18a）的工作电极的表面接触。

公开(公告)号：US08535497B2

公开(公告)日：2013-09-17

申请号：US10578275

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： G01N27/3274 ,  C12Q1/001 ,  C12Q1/006 ,  G01N33/66 ,  G01N33/728

摘要： The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The sensor for measuring a component in blood has a first analysis portion and a second analysis portion. The first analysis portion has a first electrode system (11,12) and a reagent layer (14), and the reagent layer (14) has an oxidoreductase that acts on the component and a mediator. In the first analysis portion, the component in the blood is measured by causing a redox reaction of the component with the oxidoreductase in the presence of the mediator and detecting a redox current caused when a voltage is applied by the first electrode (11,12). The second analysis portion has a working electrode and a counter electrode, and a mediator is provided on the counter electrode but not on the working electrode. In the second analysis portion, a Hct value of the blood is measured by supplying the blood to the electrode system, applying a voltage to cause a current to flow, and detecting a value of the current. Using this Hct value, the amount of the component is corrected.

公开(公告)号：US08088271B2

公开(公告)日：2012-01-03

申请号：US10578988

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/26 ,  G01N33/487

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US20100243476A1

公开(公告)日：2010-09-30

申请号：US12377514

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N27/26 ,  G01N33/50

CPC分类号： G01N27/3274 ,  G01N27/3272 ,  G01N27/413

摘要： Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) having branch parts (18a, 18b). A first reagent (13) consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (18b), and a second reagent (19) consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part (18a).

摘要翻译： 在氧化还原物质中的氧化剂基本上不与工作电极接触但与对电极接触的状态下，跨越对置电极和与血液样品接触的工作电极施加电压， 还原剂基本上不与对电极接触，而是与工作电极接触，由此还原剂和氧化剂分别被氧化和还原，以测量在氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的Hct值。 该测量可以用包括工作电极（11），对电极（12）和具有分支部分（18a，18b）的血液样本保持部分（14）的传感器芯片进行。 基本上由氧化还原物质中的氧化剂组成的第一试剂（13）被设置成与面对分支部分（18b）的对电极的表面接触，并且基本上由还原剂组成的第二试剂（19）设置在 与面向分支部（18a）的工作电极的表面接触。

公开(公告)号：US07641785B2

公开(公告)日：2010-01-05

申请号：US10954020

申请日：2004-09-28

申请人： Teppei Shinno ,  Shin Ikeda

发明人： Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/327

CPC分类号： C12Q1/001 ,  C12Q1/006 ,  G01N27/3274 ,  G01N33/66 ,  G01N33/721

摘要： A sensor for blood component analysis that can correct the effect of a hematocrit easily is provided. The sensor includes an analysis portion including a working electrode, a counter electrode, and a reagent portion. The reagent portion includes an oxidoreductase that reacts with the blood component and a mediator, and the blood component is measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode. In this sensor, the reagent portion further includes a hemolyzing agent (e.g., sodium cholate) for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.

摘要翻译： 提供了可以容易地校正血细胞比容的影响的用于血液成分分析的传感器。 传感器包括分析部分，其包括工作电极，对电极和试剂部分。 试剂部分包括与血液成分和介体反应的氧化还原酶，并且通过在介体存在下引起血液成分和氧化还原酶之间的氧化还原反应来测定血液成分，并检测由氧化还原反应产生的氧化还原电流 通过工作电极和对电极。 在该传感器中，试剂部还含有用于溶血红细胞的溶血剂（例如胆酸钠），当检测到氧化还原电流时，红细胞用溶血剂溶血，使血红蛋白释放到红细胞外 与介体反应，并且检测由该反应产生的电流以校正血细胞比容的影响。