公开(公告)号：USRE39390E1

公开(公告)日：2006-11-14

申请号：US10924334

申请日：2002-05-17

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327

摘要： There is provided a cholesterol sensor with high-accuracy and excellent response, whose object to be measured is whole blood, where plasma with which is obtained by filtering out hemocytes therein filtered in blood can rapidly reach an electrode system. In a biosensor where plasma with which is obtained by filtering out hemocytes therein filtered with by a filter is sucked into a sample solution supply pathway due to capillarity, there are formed: a first pressing part for holding a primary side portion of the filter from the bottom; a second pressing part for holding a secondary side portion of the filter from the top and the bottom; a third pressing part for holding the central portion of the filter from the top; and a void for surrounding the filter between the second pressing part and third pressing part.

摘要翻译： 提供了具有高精度和优异反应的胆固醇传感器，其测量对象为全血，等离子体<？delete-start id =“DEL-S-00001”date =“20061114”？>与<？delete -end id =“DEL-S-00001”？> <？insert-start id =“INS-S-00001”date =“20061114”？>通过过滤出<？insert-end id =“INS- S-00001“？>血细胞<？delete-start id =”DEL-S-00002“date =”20061114“？>其中过滤的<？delete-end id =”DEL-S-00002“？> 在血液中开始id =“INS-S-00002”date =“20061114”？> <？insert-end id =“INS-S-00002”？>可以快速到达电极系统。 在等离子体<？delete-start id =“DEL-S-00003”date =“20061114”？>与<？delete-end id =“DEL-S-00003”？> <？insert-start id = 通过滤出<？insert-end id =“INS-S-00003”？>血细胞<？delete-start id =“DEL-S-00004”获得的“INS-S-00003”date =“20061114”？ “date =”20061114“？>用<？delete-end id =”DEL-S-00004“？> <？insert-start id =”INS-S-00004“date =”20061114“？> ？insert-end id =“INS-S-00004”？>由于毛细作用，将过滤器吸入样品溶液供给路径，形成有：从底部保持过滤器的初级侧部的第一按压部， 用于从顶部和底部保持过滤器的次级侧部分的第二按压部分; 用于从顶部保持过滤器的中心部分的第三按压部分; 以及用于围绕第二按压部和第三按压部之间的过滤器的空隙。

公开(公告)号：US20050175509A1

公开(公告)日：2005-08-11

申请号：US10515602

申请日：2003-12-19

申请人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

发明人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

IPC分类号： C12Q1/00 ,  G01N27/30

CPC分类号： C12Q1/006 ,  C12Q1/004 ,  G01N2333/904

摘要： The present invention relates to a biosensor which comprises an electrode system including at least one pair of electrodes, at least one insulating base plate for supporting the electrode system, a first reaction layer provided at least on a working electrode of the electrode system, including an organic compound having a functional group capable of bonding or being adsorbed to an electrode and a hydrophobic hydrocarbon group, a second reaction layer provided on the first reaction layer, including an amphiphilic lipid capable of bonding or being adsorbed to a hydrophobic portion of the first reaction layer, and a reagent system carried in a two-component membrane composed of the first and second reaction layers, including at least membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase and an electron mediator.

摘要翻译： 本发明涉及一种生物传感器，其包括电极系统，该电极系统包括至少一对电极，至少一个用于支撑电极系统的绝缘基板，至少设置在电极系统的工作电极上的第一反应层， 具有能够键合或吸附到电极和疏水性烃基的官能团的有机化合物，设置在第一反应层上的第二反应层，包括能够键合或吸附到第一反应的疏水部分的两亲性脂质 以及由第一反应层和第二反应层构成的双组分膜中携载的至少包含膜结合型吡喹啉醌依赖性葡萄糖脱氢酶和电子介体的试剂系统。

公开(公告)号：US20060030028A1

公开(公告)日：2006-02-09

申请号：US10312034

申请日：2002-06-14

申请人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

发明人： Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： C12Q1/003 ,  C12Q1/004

摘要： A simple-structured biosensor is provided for measuring a substrate in a sample rapidly and highly precisely, without significant influence of a peptide included in the sample. A biosensor for measuring the substrate contained in a sample solution includes one or a plurality of insulating base plate(s) 1; a pair of electrodes including a working electrode 2 and a counter electrode 3 provided on the base plate 1; and a reagent 11 for measurement including a redox enzyme and an electron mediator. The sample solution contains a peptide, and the working electrode 2 includes a metal, and at least a part of a surface of the working electrode 2 is covered with a film 10 of an organic compound containing at least one sulfur atom.

摘要翻译： 提供了一种简单结构的生物传感器，用于在样品中包含的肽的显着影响下快速高度精确地测量样品中的底物。 用于测量包含在样品溶液中的基底的生物传感器包括一个或多个绝缘基板1; 包括设置在基板1上的工作电极2和对置电极3的一对电极; 和包含氧化还原酶和电子介体的测定用试剂11。 样品溶液含有肽，工作电极2包括金属，工作电极2的至少一部分表面被含有至少一个硫原子的有机化合物的膜10覆盖。

公开(公告)号：US20050072670A1

公开(公告)日：2005-04-07

申请号：US10478283

申请日：2002-12-27

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  C12Q1/60 ,  G01N27/403 ,  G01N33/487 ,  G01N33/49 ,  G01N27/26

CPC分类号： C12Q1/001 ,  C12Q1/006 ,  C12Q1/60 ,  G01N27/3272

摘要： In order to provide a biosensor with high accuracy and excellent response where plasma obtained by filtration of blood rapidly arrives at an electrode system, in a biosensor comprising: an insulating base plate; an electrode system having a working electrode and a counter electrode which are provided on the base plate; a reaction layer including at least oxidoreductase and an electron mediator; a sample solution supply pathway which includes the electrode system and the reaction layer and has an inlet and an air aperture; a sample solution supply part for introducing a sample solution, which is in position apart from the sample solution supply pathway; and a first filter which is disposed between the sample solution supply pathway and the sample solution supply part for filtering the sample solution, where the filtrate filtered with the first filter is supplied into the sample solution supply pathway due to capillary action, the direction in which the sample solution passes through the first filter and the direction in which the filtrate passes through the sample solution supply pathway are made cross at right angles.

摘要翻译： 为了提供具有高精度和优异响应的生物传感器，其中通过过滤血液获得的血液迅速到达电极系统，在生物传感器中包括：绝缘基板; 具有设置在基板上的工作电极和对置电极的电极系统; 至少包含氧化还原酶和电子介体的反应层; 包括电极系统和反应层并具有入口和空气孔的样品溶液供应路径; 用于引入样品溶液的样品溶液供应部件，其处于离样品溶液供给路径的位置; 以及第一过滤器，其设置在样品溶液供给路径和样品溶液供给部之间，用于过滤样品溶液，其中用第一过滤器过滤的滤液由于毛细作用而被供给到样品溶液供给通道中，其中 样品溶液通过第一过滤器，滤液通过样品溶液供应通道的方向被成直角交叉。

公开(公告)号：US06706232B2

公开(公告)日：2004-03-16

申请号：US10363589

申请日：2003-03-04

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/001

摘要： There is provided a cholesterol sensor with high-accuracy and excellent response, whose object to be measured is whole blood, where plasma with hemocytes therein filtered can rapidly reach an electrode system. In a biosensor where plasma with hemocytes therein filtered with a filter is sucked into a sample solution supply pathway due to capillarity, there are formed: a first pressing part for holding a primary side portion of the filter from the bottom; a second pressing part for holding a secondary side portion of the filter from the top and the bottom; a third pressing part for holding the central portion of the filter from the top; and a void for surrounding the filter between the second pressing part and third pressing part.

摘要翻译： 提供了具有高精度和优异反应的胆固醇传感器，其被测量对象是全血，其中血细胞在其中被过滤的血浆可以快速地达到电极系统。 在生物传感器中，其中用过滤器过滤血细胞的血浆由于毛细作用而被吸入样品溶液供应通道中，形成有：从底部保持过滤器的初级侧部分的第一按压部分; 用于从顶部和底部保持过滤器的次级侧部分的第二按压部分; 用于从顶部保持过滤器的中心部分的第三按压部分; 以及用于围绕第二按压部和第三按压部之间的过滤器的空隙。

公开(公告)号：US06740215B1

公开(公告)日：2004-05-25

申请号：US09959571

申请日：2001-10-30

申请人： Takahiro Nakaminami ,  Motokazu Watanabe ,  Shin Ikeda ,  Shiro Nankai

发明人： Takahiro Nakaminami ,  Motokazu Watanabe ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/005

摘要： A biosensor comprising an electrically insulating base plate, an electrode system containing a working electrode and a counter electrode disposed on the base plate, and a reagent system comprising at least an oxidoreductase, a hydrophilic polymer and an electron mediator, wherein the reagent system further comprises a substance having a function to convert an organic product generated by direct reaction of a substrate to be measured with the oxidoreductase to another compound.

摘要翻译： 一种生物传感器，包括电绝缘基板，包含工作电极的电极系统和设置在基板上的对电极，以及至少包含氧化还原酶，亲水性聚合物和电子介体的试剂系统，其中所述试剂系统还包括 具有将待测基质与氧化还原酶直接反应产生的有机产物转化为其他化合物的功能的物质。

公开(公告)号：US09212380B2

公开(公告)日：2015-12-15

申请号：US13392630

申请日：2010-08-30

申请人： Yoshifumi Takahara ,  Takahiro Nakaminami ,  Shin Ikeda

发明人： Yoshifumi Takahara ,  Takahiro Nakaminami ,  Shin Ikeda

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/32 ,  G01N21/84

CPC分类号： G01N27/3272 ,  C12Q1/006 ,  C12Q1/32 ,  G01N21/8483

摘要： A reagent layer of a sensor contains as a mediator a quinone compound having a hydrophilic functional group, phenanthrenequinone, and/or a phenanthrenequinone derivative. The quinone compound has a lower redox potential than a conventional mediator, so interfering substances have less effect on detection results with this sensor.

摘要翻译： 传感器的试剂层含有具有亲水性官能团的醌化合物，菲醌和/或菲醌衍生物作为介体。 醌类化合物的氧化还原电位低于常规介体，因此干扰物质对该传感器的检测结果影响较小。

公开(公告)号：US20100276303A1

公开(公告)日：2010-11-04

申请号：US12377497

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Takahiro Takasu ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Takahiro Takasu ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N33/49

CPC分类号： G01N27/3274

摘要： A voltage is applied across a counter electrode and a working electrode while an oxidant of a redox substance is in contact with the counter electrode and is not substantially in contact with the working electrode, and thereby metal that readily undergoes electrolytic oxidation forming at least part of a surface of the working electrode is oxidized while the oxidant in contact with the working electrode is reduced, such that a current produced upon the oxidation and the reduction is measured. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, a hematocrit value of a blood sample can be measured stably with satisfactory detection sensitivity. This measurement can be carried out, for example, with a sensor chip comprising a working electrode 11, a counter electrode 12, and a blood sample holder 14 in communication with a blood sample inlet 16, wherein a portion 31 of the working electrode 11 is disposed closer to the blood sample inlet 16 than a portion 32 of the counter electrode is, a surface of the portion 31 includes the metal that readily undergoes electrolytic oxidation, and a reagent 13 containing the oxidant is disposed in contact with the portion 32.

摘要翻译： 在对电极和工作电极之间施加电压，而氧化还原物质的氧化剂与对电极接触并且基本上不与工作电极接触，从而容易经历电解氧化形成的金属至少部分 工作电极的表面被氧化，而与工作电极接触的氧化剂减少，从而测量氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的血细胞比容值。 该测定例如可以使用包含与血液检体入口16连通的工作电极11，对电极12和血液检体保持器14的传感器芯片进行，其中工作电极11的部分31为 比对置电极的部分32更靠近血液采样入口16设置，部分31的表面包括容易经历电解氧化的金属，并且含有氧化剂的试剂13设置成与部分32接触。

公开(公告)号：US07235170B2

公开(公告)日：2007-06-26

申请号：US10344671

申请日：2002-05-13

申请人： Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Shiro Nankai

发明人： Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： C12Q1/26

CPC分类号： C12Q1/006 ,  C12Q1/001 ,  C12Q1/54

摘要： A biosensor that is highly responsive and capable of rapid and highly sensitive quantification of a specific component contained in a sample is provided. The biosensor of this invention comprises: an electrically insulating base plate; an electrode system comprising a working electrode and a counter electrode disposed on the base plate; and a reagent system comprising an oxidoreductase which catalyzes the oxidation reaction of glucose, gluconolactonase and a buffer. The buffer is selected from the group consisting of phthalic acid and its salts, maleic acid and its salts, succinic acid and its salts, phospholic acid and its salts, acetic acid and its salts, boric acid and its salts, citric acid and its salts, glycine, tris(hydroxymethyl)aminomethane, piperazine-N,N′-bis(2-ethane sulfonic acid) and the like.

摘要翻译： 提供了一种生物传感器，其具有高响应性并且能够对包含在样品中的特定成分进行快速和高灵敏度的定量。 本发明的生物传感器包括：电绝缘基板; 电极系统，包括设置在所述基板上的工作电极和对置电极; 以及包含催化葡萄糖，葡糖酸内切酶和缓冲液的氧化反应的氧化还原酶的试剂体系。 缓冲剂选自邻苯二甲酸及其盐，马来酸及其盐，琥珀酸及其盐，磷酸及其盐，乙酸及其盐，硼酸及其盐，柠檬酸及其盐 ，甘氨酸，三（羟甲基）氨基甲烷，哌嗪-N，N'-双（2-乙磺酸）等。

公开(公告)号：US08691072B2

公开(公告)日：2014-04-08

申请号：US12377514

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N27/26

CPC分类号： G01N27/3274 ,  G01N27/3272 ,  G01N27/413

摘要： Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) having branch parts (18a, 18b). A first reagent (13) consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (18b), and a second reagent (19) consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part (18a).

摘要翻译： 在氧化还原物质中的氧化剂基本上不与工作电极接触但与对电极接触的状态下，跨越对置电极和与血液样品接触的工作电极施加电压， 还原剂基本上不与对电极接触，而是与工作电极接触，由此还原剂和氧化剂分别被氧化和还原，以测量在氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的Hct值。 该测量可以用包括工作电极（11），对电极（12）和具有分支部分（18a，18b）的血液样本保持部分（14）的传感器芯片进行。 基本上由氧化还原物质中的氧化剂组成的第一试剂（13）被设置成与面对分支部分（18b）的对电极的表面接触，并且基本上由还原剂组成的第二试剂（19）设置在 与面向分支部（18a）的工作电极的表面接触。