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公开(公告)号：US06508923B1
公开(公告)日：2003-01-21
申请号：US09566976
申请日：2000-05-09
IPC分类号： C02F140
CPC分类号： G01N27/44782 , G01N27/44721
摘要： A data processing part comprises a time-series data production part for producing time-series data as to each capillary column from a scan waveform obtained by an optical measuring part, further comprises a correction data storage part for storing correction data indicating the relation between the number of data points of saturated parts and light intensity data obtained on the assumption that a peak is unsaturated as to a saturated scan waveform peak and a saturated data correction part obtaining light intensity data as to the saturated peak included in the scan waveform on the basis of the correction data stored in the correction data storage part, and employs the light intensity data obtained by the saturated data correction part as the time-series data.
摘要翻译： 一种数据处理部分包括一个时间序列数据生成部分，用于根据由光学测量部分获得的扫描波形来产生关于每个毛细管柱的时间序列数据，还包括校正数据存储部分，用于存储指示 基于峰值对饱和扫描波形峰值不饱和而获得的饱和部分的数据点数和光强度数据的数量以及饱和数据校正部分获得关于扫描波形中包括的饱和峰值的光强度数据 存储在校正数据存储部分中的校正数据，并且采用由饱和数据校正部分获得的光强度数据作为时间序列数据。
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公开(公告)号：US06458556B1
公开(公告)日：2002-10-01
申请号：US08899393
申请日：1997-07-23
IPC分类号： C12N900
CPC分类号： C12N9/1276 , C12N9/1252 , C12N9/22 , C12N9/96
摘要： A method for enhancing activity of enzyme at an elevated temperature which comprises adding a substance exhibiting chaperone function such as a saccharide to a reaction mixture containing the enzyme. The method can improve activity of enzymes more easily and more effectively and hence afford increased enzyme activity at an elevated temperature.
摘要翻译： 一种在升高的温度下增强酶的活性的方法，其包括在含有该酶的反应混合物中加入显示伴侣功能的物质如糖。 该方法可以更容易且更有效地提高酶的活性，从而在升高的温度下提高酶的活性。
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公开(公告)号：US06265569B1
公开(公告)日：2001-07-24
申请号：US09263520
申请日：1999-03-05
IPC分类号： C07H1900
CPC分类号： C07H19/04
摘要： Disclosed are 3′-deoxyribonucleotide derivatives represented by the following general formula [I]: Q—V—(CH2)n—NH—R [I] wherein Q represents a 3′-deoxyribonucleotide residue, n represents an integer not less than 4, V represents —C≡C— or —CH═CH—, and R represents a fluorescent group. The above 3′-deoxyribonucleotide derivatives are derivatives with improved rates for incorporation using RNA polymerases, which are useful as terminators in the DNA sequence determination methods utilizing RNA polymerases.
摘要翻译： 公开了由以下通式[I]表示的3'-脱氧核糖核苷酸衍生物：其中Q表示3'-脱氧核糖核苷酸残基，n表示不小于4的整数，V表示-C = C-或-CH = CH-， R代表荧光基团。上述3'-脱氧核糖核苷酸衍生物是使用RNA聚合酶掺入率提高的衍生物，其可用作使用RNA聚合酶的DNA序列测定方法中的终止子。
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公开(公告)号：US06258542B1
公开(公告)日：2001-07-10
申请号：US09388917
申请日：1999-09-02
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , Y10S435/912
摘要： In order to make possible to preserve promptly and efficiently a DNA and to distribute the same without taking much labor and much time, a DNA solution is allowed to adhere to a sheet-like support having a prescribed thickness, and the DNA solution which has been allowed to adhere to the support is dried to fix the DNA onto the support.
摘要翻译： 为了能够迅速且有效地保存DNA并且分发相同的DNA而不需要花费太多的劳动和时间，可以使DNA溶液粘附到具有规定厚度的片状载体上，并且已经将DNA溶液 允许粘附到支持物上以将DNA固定在载体上。
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公开(公告)号：US06221599B1
公开(公告)日：2001-04-24
申请号：US09414531
申请日：1999-10-08
IPC分类号： C12Q168
CPC分类号： C12N15/1096 , C12Q1/6844 , C12Q2527/101 , C12Q2521/107 , C12Q2527/125
摘要： A method for preparing a cDNA from a mRNA using a reverse transcriptase wherein reverse transcription is performed at a temperature at which the mRNA does not take a secondary structure, for example, at a temperature of 45° C. or more. The method is performed, for example, using a heat-labile reverse transcriptase in the presence of a substance exhibiting chaperone function having chaperone function such as saccharides. The method is performed, for example, in the presence of metal ions necessary for activation of the reverse transcriptase and a chelating agent for the metal ions such as a deoxynucleotide triphosphate. The method is capable of reverse transcription over the full length of mRNA template even if the mRNA is a long chain mRNA and, as a result, producing a full length cDNA.
摘要翻译： 使用逆转录酶从mRNA制备cDNA的方法，其中在mRNA不采取二级结构的温度下进行逆转录，例如在45℃或更高的温度下进行。 该方法例如在具有伴侣伴侣功能的物质的存在下使用热不稳定的逆转录酶，例如糖类。 该方法例如在活化逆转录酶所需的金属离子和金属离子螯合剂如脱氧核苷酸三磷酸的存在下进行。 该方法能够在mRNA模板的全长上进行逆转录，即使mRNA是长链mRNA，因此产生全长cDNA。
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公开(公告)号：US5916775A
公开(公告)日：1999-06-29
申请号：US877874
申请日：1997-06-18
CPC分类号： C12Q1/6806 , C12N15/101 , C12N15/1017
摘要： Disclosed is a method for collecting DNA by lysing microbial cells, adsorbing released DNA on a carrier and collecting the DNA adsorbed on the carrier, which method comprises the following steps of (1) lysing the microbial cells in the presence of the carrier so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA from the carrier, and eluting the DNA adsorbed on the carrier with a solution for eluting DNA and collecting eluted DNA, or (2) feeding microbial cells into a column comprising the carrier provided on a membrane filter capable of retaining a solution and permeating the solution when aspirated, lysing the microbial cells in the column so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA in the previous step from the column by aspiration, and feeding a solution for eluting DNA into the column and aspirating to collect the DNA adsorbed on the carrier. The methods of the present invention enable collection of DNA by the chaotropic ion method employing an apparatus with simpler structure and fewer operations.
摘要翻译： 公开了通过溶解微生物细胞，将载体上释放的DNA吸附并收集载体上的DNA收集DNA的方法，该方法包括以下步骤：（1）在载体存在下裂解微生物细胞，使得 通过裂解细胞获得的DNA被吸附到载体上，分离用于裂解细胞并从载体吸附DNA的溶液，并用洗脱DNA并收集洗脱的DNA的溶液洗脱载体上的DNA，或（2）喂养微生物细胞 进入包含载体的柱，该载体提供在能够保留溶液并在吸出时渗透溶液的膜过滤器上，裂解塔中的微生物细胞，使得通过裂解细胞获得的DNA被吸附到载体上，用于裂解细胞的分离溶液 并通过抽吸从柱上吸附DNA，并将用于将DNA洗脱的溶液供入柱中并吸出以收集DNA 吸附在载体上。 本发明的方法能够利用离液力离子法采用具有更简单结构和更少操作的装置来收集DNA。
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47.发明授权Pretreatment method of biological sample, detection method of RNA, and pretreatment kit 有权生物样品预处理方法，RNA检测方法及预处理试剂盒公开(公告)号：US09518901B2
公开(公告)日：2016-12-13
申请号：US13807605
申请日：2012-06-28
申请人： Yoshihide Hayashizaki , Kengo Usui , Saori Goda , Kazuhito Nomura , Yuki Kawai
发明人： Yoshihide Hayashizaki , Kengo Usui , Saori Goda , Kazuhito Nomura , Yuki Kawai
CPC分类号： G01N1/34 , C12N15/1003 , C12Q1/6806
摘要： The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.
摘要翻译： 本发明提供一种能够及时且简单地检测RNA的预处理方法。 例如，通过将铁离子和碳酸根离子加入到含有人类鼻粘膜的生物样品中，可抑制由于存在于人类鼻粘膜中的乳铁蛋白引起的RNA降解活性。 通过预处理的生物样品，RNA病毒基因可以通过逆转录酶扩增。 铁离子和碳酸根离子也可以抑制人类鼻粘膜中包含的溶菌酶C的逆转录酶抑制。 此外，优选通过向包含人类鼻粘膜的生物样品中加入SDS来除去RNA病毒的包膜。
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48.发明授权Malignant tumor cell suppressor protein, malignant tumor cell suppressor gene, maligant tumor cell suppressive viral vector, and kit using the same 有权恶性肿瘤细胞抑制蛋白，恶性肿瘤细胞抑制基因，恶性肿瘤细胞抑制病毒载体和使用其的试剂盒公开(公告)号：US08956606B2
公开(公告)日：2015-02-17
申请号：US11922933
申请日：2006-06-30
IPC分类号： C12N15/861 , C12N15/85 , C12N15/63 , C12N5/10
CPC分类号： C07K14/4703 , A61K38/00 , A61K48/00
摘要： A malignant tumor cell suppressor protein (a) or (b): (a) a protein comprising an amino acid sequence represented by SEQ ID No. 1; or (b) a protein comprising an amino acid sequence represented by SEQ ID No. 1, wherein one or more amino acid are deleted, substituted or added in the amino acid sequence set forth in SEQ ID No. 1.
摘要翻译： 恶性肿瘤细胞抑制蛋白（a）或（b）：（a）包含SEQ ID No.1所示的氨基酸序列的蛋白质; 或（b）包含SEQ ID No.1所示的氨基酸序列的蛋白质，其中一个或多个氨基酸被缺失，取代或加入到SEQ ID No.1所示的氨基酸序列中。
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公开(公告)号：US20110243910A1
公开(公告)日：2011-10-06
申请号：US13058970
申请日：2009-07-07
IPC分类号： A61K38/45 , C12N9/96 , G01N33/573 , C12N5/071 , C12Q1/68 , C12M1/40 , A61P19/02 , A61P35/00 , A61P35/02 , A61P11/06 , A61P17/14 , A61P7/06 , A61P3/00 , C07K14/00
CPC分类号： C12N9/1276 , A61K38/00 , C12N9/127 , C12Y207/07048 , C12Y207/07049
摘要： The invention provides compositions comprising a TERT-RMRP or TERT-RNA complex and methods of treating subjects with genetic diseases in which gene silencing is either increased by administering the compositions of the invention or decreased by administering an inhibitor of the RNA-dependent RNA polymerase (RdRP) activity of these compositions. Moreover, the invention provides methods of screening for agonists and antagonists of RdRP activity and TERT-RMRP complex formation. Finally, the invention provides a method of identifying a RNA molecule that forms a complex with a TERT polypeptide and has RdRP activity.
摘要翻译： 本发明提供了包含TERT-RMRP或TERT-RNA复合物的组合物和治疗具有遗传疾病的受试者的方法，其中通过施用本发明的组合物或通过施用RNA依赖性RNA聚合酶的抑制剂降低基因沉默 RdRP）活性。 此外，本发明提供筛选RdRP活性和TERT-RMRP复合物形成的激动剂和拮抗剂的方法。 最后，本发明提供了鉴定与TERT多肽形成复合物并具有RdRP活性的RNA分子的方法。
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公开(公告)号：US07374882B2
公开(公告)日：2008-05-20
申请号：US10432991
申请日：2001-11-11
CPC分类号： C12N15/115 , C07H19/10 , C07H19/20 , C07H21/00 , C12N2310/33 , C12Q1/6869 , C40B30/04 , G01N33/6845 , C12Q2525/117
摘要： Aptamers are nucleic acids and similar molecules, such as peptide-nucleic acids, that specifically bind to a ligand such as a protein or peptide. The present invention provides aptamers comprising at least one base capable of base pairing and different from the standard Watson-Crick bases. The present invention also relates to a method for preparation of such aptamers and to methods for sequencing nucleic acids that comprise at least one base capable of base pairing and different from the standard Watson-Crick bases.
摘要翻译： 适体是核酸和类似分子，例如肽 - 核酸，其特异性结合配体如蛋白质或肽。 本发明提供了包含至少一个能够碱基配对并且不同于标准沃森 - 克里克碱基的碱基的适体。 本发明还涉及这种适体的制备方法以及核酸测序方法，所述方法包括至少一个能够碱基配对且不同于标准沃森 - 克里克碱基的碱基。
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