公开(公告)号：US5683891A

公开(公告)日：1997-11-04

申请号：US436462

申请日：1995-05-26

申请人： Kiyoto Nishiyama ,  Yuji Ishikawa ,  Kazuhiko Kimachi ,  Hiroaki Maeda ,  Sachio Tokiyoshi

发明人： Kiyoto Nishiyama ,  Yuji Ishikawa ,  Kazuhiko Kimachi ,  Hiroaki Maeda ,  Sachio Tokiyoshi

IPC分类号： C07K16/00 ,  C12N15/85 ,  C12P21/08 ,  C07K16/44 ,  C12N15/06 ,  C12N15/13

CPC分类号： C12N15/85 ,  C07K16/00 ,  C12N2840/20 ,  C12N2840/44

摘要： A novel method for production of an exogenous protein is provided, the method being suitable for expression of an exogenous protein, especially a recombinant antibody etc., in an eucaryotic cell by utilizing a genetic recombination technique. That is, a novel method for production of an exogenous protein is provided which allows for culture of a cell in which an exogenous gene is introduced in a serum-free medium and an efficient production of an exogenous protein, by using, as a host cell for expression, a fused cell which is prepared by fusing a mouse myeloma and a lymphatic cell and which can be cultured in a serum-free medium.

摘要翻译： PCT No.PCT / JP95 / 01723 Sec。 371日期：1995年5月26日 102（e）日期1995年5月26日PCT提交1993年11月25日PCT公布。 公开号WO94 / 12658 日期1994年6月9日提供了一种生产外源蛋白质的新方法，该方法适用于通过利用遗传重组技术在真核细胞中表达外源蛋白，特别是重组抗体等。 也就是说，提供了一种用于生产外源蛋白质的新方法，其允许通过使用作为宿主细胞来培养外源基因导入无血清培养基和有效产生外源蛋白质的细胞 用于表达融合细胞，其通过融合小鼠骨髓瘤和淋巴细胞并可在无血清培养基中培养而制备。

公开(公告)号：US5593861A

公开(公告)日：1997-01-14

申请号：US306520

申请日：1994-09-15

申请人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

发明人： Hiroaki Maeda ,  Yasuyuki Eda ,  Kazuhiko Kimachi ,  Yoichi Ono ,  Sachio Tokiyoshi

IPC分类号： A61K39/00 ,  C07K16/46 ,  C12N5/26 ,  C12N15/13 ,  C12N15/00 ,  C07H21/04 ,  C12P21/04 ,  C12P21/06

CPC分类号： C07K16/462 ,  A61K39/00 ,  C07K2317/24

摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.

摘要翻译： 一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段 的犬免疫球蛋白; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

公开(公告)号：US5560914A

公开(公告)日：1996-10-01

申请号：US501751

申请日：1995-07-12

申请人： Mamdooh H. Ghoneum ,  Hiroaki Maeda

发明人： Mamdooh H. Ghoneum ,  Hiroaki Maeda

IPC分类号： C12N9/14 ,  A61K31/715 ,  A61K36/899 ,  A61K39/39 ,  A61P37/04 ,  C08B37/00 ,  C12P19/04 ,  C12P19/20 ,  C12R1/645 ,  C12R1/69 ,  A61K35/78 ,  C12N1/14

CPC分类号： A61K39/39 ,  Y10S435/911

摘要： In a method of producing an immunopotentiator, a plant tissue material is extracted with hot water, the insoluble material is filtered, and the filtrate thereof is treated with glucoamylase to decompose a starch, thereby obtaining a water-soluble polysaccharide. Ammonium sulfate is added in a culture filtrate obtained by culturing any one of Asp Oryzae of the Aspergillaceae and Lentinus edodes of the Basidiomycetes to obtain an enzyme complex from the precipitate. The water-soluble polysaccharide and the enzyme complex are added together to cause them to react at a pH of 4.5 for 30 to 60 minutes and then at a pH of 6.0 for 30 to 60 minutes, thereby biologically modifying the water-soluble polysaccharide.

摘要翻译： 在制造免疫增强剂的方法中，用热水提取植物组织物质，过滤不溶物，将其滤液用葡糖淀粉酶处理，分解淀粉，得到水溶性多糖。 将硫酸铵加入通过培养曲霉的Asp Oryzae和担子菌的香菇中的任一种获得的培养滤液中，从沉淀物中获得酶复合物。 将水溶性多糖和酶复合物加入到一起，使它们在4.5的pH下反应30至60分钟，然后在6.0的pH下反应30至60分钟，从而对水溶性多糖进行生物修饰。

公开(公告)号：US5139122A

公开(公告)日：1992-08-18

申请号：US676313

申请日：1991-03-28

申请人： Hiroaki Maeda ,  Takahiro Misu ,  Masahiro Omura ,  Yuzo Masuda ,  Toshihiro Kobayashi

发明人： Hiroaki Maeda ,  Takahiro Misu ,  Masahiro Omura ,  Yuzo Masuda ,  Toshihiro Kobayashi

IPC分类号： F16H45/02

CPC分类号： F16H45/02 ,  F16H2045/0226

摘要： A lock-up clutch in a torque converter includes a front cover connected to an input shaft, a piston disposed between the front cover and a torque converter mechanism that can be axially moved on an input shaft and can be engaged or disengaged with the front cover. A first member is disposed between the piston and the torque converter mechanism, a second member is provided at both sides of the first member and the second member is rotatable with respect to the first member for a first predetermined rotational angle. A first elastic member is disposed between the first member and the second member for elastically connecting the first member and the second member with each other and a second elastic member is provided inwardly of the first elastic member for elastically connecting the first member at a second predetermined rotational angle which is less than the first predetermined rotational angle. A stopper member is provided at the outer periphery of the second member for slowing the rotational speed of the first member with respect to the second member by stopping the projection in the rotational direction at a third predetermined rotational angle which is less than the first predetermined rotational angle and more than the second predetermined rotational angle.

摘要翻译： 变矩器中的锁止离合器包括连接到输入轴的前盖，设置在前盖和变矩器机构之间的活塞，其可在输入轴上轴向移动并且可以与前盖接合或分离 。 第一构件设置在活塞和变矩器机构之间，第二构件设置在第一构件的两侧，第二构件可相对于第一构件旋转第一预定旋转角。 第一弹性构件设置在第一构件和第二构件之间，用于将第一构件和第二构件彼此弹性地连接，并且第二弹性构件设置在第一弹性构件的内部，用于以第二预定的弹性连接第一构件 旋转角度小于第一预定旋转角度。 在第二构件的外周设置有止动构件，用于通过以小于第一预定旋转的第三预定旋转角度沿着旋转方向停止突起来减慢第一构件相对于第二构件的旋转速度 角度大于第二预定旋转角度。

公开(公告)号：US4885960A

公开(公告)日：1989-12-12

申请号：US238610

申请日：1988-08-31

申请人： Hiroaki Maeda ,  Masahiko Nakamura

发明人： Hiroaki Maeda ,  Masahiko Nakamura

IPC分类号： F16H61/04 ,  F16H59/22 ,  F16H59/44 ,  F16H61/10 ,  F16H61/20

CPC分类号： F16H61/20 ,  F16H59/22 ,  F16H59/44 ,  Y10T477/681

摘要： A creep phenomenon of an automotive vehicle is prevented by an arrangement which is provided with an anti-creep mode which is operative to change the gear automatically to a speed range having a relatively low speed reduction ratio when the vehicle is at rest. A double engagement within the transmission and resulting oscillations which would occur when a special operation is performed is prevented. At this end, a time delay is provided before shifting to the anti-creep mode when the vehicle is at rest and the throttle valve opening of an engine is equal to or less than a given value.

摘要翻译： 通过设置有抗蠕变模式的装置来防止机动车辆的蠕变现象，所述抗蠕变模式可在车辆静止时自动地将齿轮改变到具有相对低的减速比的速度范围。 防止在执行特殊操作时发生的传输和产生的振荡中的双重接合。 为此，在车辆静止而发动机的节气门开度为规定值以下时，在变换为抗蠕变模式之前，提供时间延迟。

公开(公告)号：US4706549A

公开(公告)日：1987-11-17

申请号：US895825

申请日：1986-08-12

申请人： Toshiyuki Inagaki ,  Hiroaki Maeda

发明人： Toshiyuki Inagaki ,  Hiroaki Maeda

IPC分类号： F16H61/02 ,  F16H61/06 ,  F15B11/08

CPC分类号： F16H61/068 ,  F16H61/0272

摘要： An oil pressure control system for a power transmission which includes an oil pump, a regulator valve, an inching valve, a selector valve, a change over valve, a modulator valve, a clutch servo, a first oil passage for communicating the oil pump with the selector valve via the regulator valve and the inching valve, a second oil passage for communicating the selector valve with the change over valve and the clutch servo, a third oil passage for communicating the change over valve with one end of the modulator valve and a fourth oil passage for communicating the first oil passage located between the inching valve and the selector valve with the other side of the modulator valve. The system permits a smooth, controlled operation of the clutch servo.

摘要翻译： 一种用于动力传递的油压控制系统，包括油泵，调节阀，微动阀，选择阀，换向阀，调节阀，离合器伺服器，用于将油泵与 所述选择阀经由所述调节阀和所述微动阀，用于使所述选择阀与所述换向阀和所述离合器伺服连通的第二油路，用于使所述换向阀与所述调节阀的一端连通的第三油路， 第四油路，用于使位于调节阀的另一侧的位于微动阀和选择阀之间的第一油路连通。 该系统允许离合器伺服的平稳控制操作。

公开(公告)号：US4170153A

公开(公告)日：1979-10-09

申请号：US787931

申请日：1977-04-15

申请人： Kiyohumi Mizuno ,  Hiroaki Maeda ,  Shigeo Takahashi

发明人： Kiyohumi Mizuno ,  Hiroaki Maeda ,  Shigeo Takahashi

IPC分类号： F16H47/04 ,  F16H61/42 ,  F16H61/46 ,  F16H61/462 ,  B60K21/00 ,  B60K41/04

CPC分类号： F16H61/42 ,  F16H47/04 ,  F16H61/46 ,  F16H61/462 ,  F16H2037/0886 ,  Y10T477/637

摘要： In a hydromechanical transmission, low and high speed drive power trains are selectively completed in response to variation of the speed ratio between an input shaft and an output shaft. The transmission comprises a speed ratio detecting valve for detecting lower and higher limits of a predetermined speed ratio and a clutch control valve for conducting the switchover between the low and high speed drive power trains with a predetermined time lag after the actual speed ratio reaches a range defined by the lower and higher limits of the predetermined speed ratio.

摘要翻译： 在液压机械变速器中，响应于输入轴和输出轴之间的速比的变化，选择性地完成低速和高速驱动动力系。 变速器包括用于检测预定速比的下限和上限的变速比检测阀和用于在实际变速比达到范围之后以预定的时间滞后在低速和高速驱动功率列之间进行切换的离合器控制阀 由预定速比的下限和上限定义。

公开(公告)号：US10544425B2

公开(公告)日：2020-01-28

申请号：US13976526

申请日：2011-12-26

申请人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

发明人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

IPC分类号： C12N15/82 ,  C12Q1/6895

摘要： By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.

公开(公告)号：US20130041137A1

公开(公告)日：2013-02-14

申请号：US13271761

申请日：2011-10-12

申请人： Kenji SOEJIMA ,  Noriko Mimura ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Takayoshi Hamamoto ,  Tomohiro Nakagaki

发明人： Kenji SOEJIMA ,  Noriko Mimura ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Takayoshi Hamamoto ,  Tomohiro Nakagaki

IPC分类号： C07K16/40

CPC分类号： C12N9/6489 ,  A61K31/711 ,  A61K38/00 ,  C12N9/50 ,  C12N9/6421

摘要： This invention is intended to isolate and identify a vWF-specific cleaving protease. The vWF-specific cleaving protease cleaves a bond between residues Tyr 842 and Met 843 of vWF and comprises a polypeptide chain having Leu-Leu-Val-Ala-Val (SEQ ID NO: 1) as a partial sequence, and more preferably comprises a polypeptide chain having the partial N-terminal amino acid sequence of a mature protein, Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val (SEQ ID NO: 2), and having a molecular weight of 105 to 160 kDa in SDS-PAGE under reducing or non-reducing conditions. Isolation and identification of this vWF-specific cleaving protease have led to the possibility of replacement therapy for patients having diseases resulting from a deficiency of the protease, such as thrombotic thrombocytopenic purpura.

摘要翻译： 本发明旨在分离和鉴定vWF特异性切割蛋白酶。 vWF特异性切割蛋白酶切割vWF的残基Tyr 842和Met 843之间的键，并且包含具有Leu-Leu-Val-Ala-Val（SEQ ID NO：1）作为部分序列的多肽链，更优选包含 具有成熟蛋白的部分N-末端氨基酸序列的Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val（SEQ ID NO：2） ，并且在还原或非还原条件下，在SDS-PAGE中具有105至160kDa的分子量。 这种vWF特异性切割蛋白酶的分离和鉴定已经导致了患有由蛋白酶缺乏引起的疾病的患者（例如血栓性血小板减少性紫癜）的替代疗法的可能性。

公开(公告)号：US08067221B2

公开(公告)日：2011-11-29

申请号：US12103899

申请日：2008-04-16

申请人： Kenji Soejima ,  Noriko Mimura ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Takayoshi Hamamoto ,  Tomohiro Nakagaki

发明人： Kenji Soejima ,  Noriko Mimura ,  Hiroaki Maeda ,  Chikateru Nozaki ,  Takayoshi Hamamoto ,  Tomohiro Nakagaki

IPC分类号： C12N9/48 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/37 ,  C12Q1/00 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/6489 ,  A61K31/711 ,  A61K38/00 ,  C12N9/50 ,  C12N9/6421

摘要： This invention is intended to isolate and identify a vWF-specific cleaving protease. The vWF-specific cleaving protease cleaves a bond between residues Tyr 842 and Met 843 of vWF and comprises a polypeptide chain having Leu-Leu-Val-Ala-Val (SEQ ID NO: 1) as a partial sequence, and more preferably comprises a polypeptide chain having the partial N-terminal amino acid sequence of a mature protein, Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val (SEQ ID NO: 2), and having a molecular weight of 105 to 160 kDa in SDS-PAGE under reducing or non-reducing conditions. Isolation and identification of this vWF-specific cleaving protease have led to the possibility of replacement therapy for patients having diseases resulting from a deficiency of the protease, such as thrombotic thrombocytopenic purpura.

摘要翻译： 本发明旨在分离和鉴定vWF特异性切割蛋白酶。 vWF特异性切割蛋白酶切割vWF的残基Tyr 842和Met 843之间的键，并且包含具有Leu-Leu-Val-Ala-Val（SEQ ID NO：1）作为部分序列的多肽链，更优选包含 具有成熟蛋白的部分N-末端氨基酸序列的Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val（SEQ ID NO：2） ，并且在还原或非还原条件下，在SDS-PAGE中具有105至160kDa的分子量。 这种vWF特异性切割蛋白酶的分离和鉴定已经导致了患有由蛋白酶缺乏引起的疾病的患者（例如血栓性血小板减少性紫癜）的替代疗法的可能性。