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79 条记录 (耗时 0.031 秒)

    Nucleic acid analysis using non-templated nucleotide addition
    41.
    发明申请
    Nucleic acid analysis using non-templated nucleotide addition 失效
    使用非模板核苷酸添加的核酸分析

    公开(公告)号：US20020142342A1

    公开(公告)日：2002-10-03

    申请号：US10109104

    申请日：2002-03-27

    申请人： PE Corporation (NY)

    发明人： Dong Wei ,  Danielle Bishop

    IPC分类号： C12Q001/68 ,  C12P019/34

    CPC分类号： C12P19/34 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q2525/186 ,  C12Q2521/131 ,  C12Q2545/113 ,  C12Q2533/101

    摘要： One embodiment of the invention is a method of producing an oligonucleotide extended by a single nucleotide base. An oligonucleotide and an extension terminating nucleotide are mixed with an enzyme having terminal transferase activity. The reaction produces an oligonucleotide extended by a single base. The extended oligonucleotide may be used as a size standard for single base extension reactions. Another embodiment of the invention is a method of producing a mixture of oligonucleotides extended by different single bases. An oligonucleotide, a first extension terminating nucleotide, and a second extension terminating nucleotide are mixed with an enzyme having terminal transferase activity. The first and second extension terminating nucleotides comprise different nucleotide bases and are labeled with different labels. The identity of the different extension terminating nucleotides (and hence the extended oligonucleotides) may be ascertained by reference to the specific label incorporated.

    摘要翻译： 本发明的一个实施方案是制备由单核苷酸碱基延伸的寡核苷酸的方法。 将寡核苷酸和延伸终止核苷酸与具有末端转移酶活性的酶混合。 该反应产生由单个碱基延伸的寡核苷酸。 延伸的寡核苷酸可以用作单碱基延伸反应的大小标准。 本发明的另一个实施方案是制备由不同单碱基延伸的寡核苷酸的混合物的方法。 将寡核苷酸，第一延伸终止核苷酸和第二延伸终止核苷酸与具有末端转移酶活性的酶混合。 第一和第二延伸终止核苷酸包含不同的核苷酸碱基，并用不同的标记进行标记。 不同延伸的终止核苷酸（因此延伸的寡核苷酸）的身份可以通过引用的特定标记来确定。

    Estrogen receptor alpha variants and methods of detection thereof
    42.
    发明申请
    Estrogen receptor alpha variants and methods of detection thereof 审中-公开
    雌激素受体α变体及其检测方法

    公开(公告)号：US20020123095A1

    公开(公告)日：2002-09-05

    申请号：US09933267

    申请日：2001-08-21

    申请人： PE CORPORATION (NY)

    发明人： Francis Kalush ,  Michael J. Cassel ,  Stuart Soo-In Hwang ,  Emily S. Winn-Deen

    IPC分类号： C07K014/705 ,  C07H021/04 ,  C07K016/28 ,  C12P021/02 ,  C12N005/06

    CPC分类号： C12Q1/68 ,  C07K14/70567 ,  C07K14/721 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/172

    摘要： The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor alpha genes and novel polymorphism/haplotypes in the estrogen receptor gene/protein. Such polymorphism/haplotypes can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorder, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences and sequence polymorphism/haplotypes in the ESR-alpha genes, methods of detecting these sequences/polymorphism/haplotypes in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.

    摘要翻译： 本发明基于对来自人6号染色体和cDNAs的基因组DNA进行测序，以确定雌激素受体基因/蛋白质中雌激素受体α基因的基因组结构和新型多态性/单体型。 这样的多态性/单元型可导致由变体雌激素受体介导/调节的各种疾病，例如对癌症，骨质疏松症，心血管病症等的易感性。基于该测序方法，本发明提供基因组核苷酸序列， ESR-α基因中的cDNA序列，氨基酸序列和序列多态性/单倍型，检测样品中这些序列/多态/单倍型的方法，确定具有或发展由变体雌激素受体介导的病症的风险的方法和方法 筛选用于治疗由变体雌激素受体介导的病症的化合物。

    Device and method for thermal cycling
    50.
    发明申请
    Device and method for thermal cycling 有权
    用于热循环的装置和方法

    公开(公告)号：US20030143723A1

    公开(公告)日：2003-07-31

    申请号：US10058927

    申请日：2002-01-30

    申请人： PE Corporation (NY)

    发明人： Donald R. Sandell

    IPC分类号： C12M001/34

    CPC分类号： B01L7/52 ,  B01L9/523 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1805 ,  C12Q1/686

    摘要： A thermal cycling device for performing nucleic acid amplification on a plurality of biological samples positioned in a sample well tray. The thermal cycling device includes a sample block assembly, an optical detection system, and a sample well tray holder configured to hold the sample well tray. The sample block assembly is adapted for movement between a first position permitting the translation of the sample well tray into alignment with sample block assembly, and a second position, upward relative to the first position, where the sample block assembly contacts the sample well tray. A method of performing nucleic acid amplification on a plurality of biological samples positioned in a sample well tray in a thermal cycling device is also provided.

    摘要翻译： 一种热循环装置，用于对位于样品井托盘中的多个生物样品进行核酸扩增。 热循环装置包括样品块组件，光学检测系统和构造成保持样品井托盘的样品孔盘托架。 样品块组件适于在允许样品井盘的平移与样品块组件对准的第一位置和相对于第一位置向上的第二位置之间移动，其中样品块组件接触样品井盘。 还提供了对位于热循环装置中的样品阱托盘中的多个生物样品进行核酸扩增的方法。