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    Method of sorting vesicle-entrapped, coupled nucleic acid-protein displays
    2.
    发明申请
    Method of sorting vesicle-entrapped, coupled nucleic acid-protein displays 审中-公开
    分离囊泡夹带的偶联核酸蛋白质显示器的方法

    公开(公告)号：US20040265835A1

    公开(公告)日：2004-12-30

    申请号：US10610024

    申请日：2003-06-30

    发明人： David M. Lemaster ,  James H. Jett

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12P021/04 ,  C12N009/00 ,  C12N001/18

    CPC分类号： C40B40/02 ,  C07H21/04 ,  C12N15/1037

    摘要： A method for identifying and sorting variants of a chosen enzyme is disclosed. Enzyme variants of a chosen enzyme are obtained and then linked to their corresponding genetic code through any of a family of suitable surface display methods. The enzyme variants, now displayed on the surface of a biological particle such as a phage, virus, yeast, or bacterium are then encapsulated in a vesicle containing an enzyme activity-sensitive assay reagent. The enzyme variant is thus exposed to the assay reagent, and displays a signal using the enzyme activity-sensitive assay reagent in a manner proportionate to the levels of activity of the enzyme, thus rendering the vesicles suitable for mechanical sorting based on these levels. The vesicles are then sorted using methods known in the art to isolate those variants exhibiting possibly beneficial variations in enzyme function.

    摘要翻译： 公开了用于鉴定和分选所选酶的变体的方法。 获得选择的酶的酶变体，然后通过任何一种合适的表面显示方法与其相应的遗传密码连接。 然后将现在显示在诸如噬菌体，病毒，酵母或细菌的生物颗粒的表面上的酶变体包封在含有酶活性敏感性测定试剂的囊泡中。 因此，酶变体暴露于测定试剂，并且以与酶的活性水平成比例的方式使用酶活性敏感的测定试剂显示信号，从而使得囊泡适合于基于这些水平的机械分选。 然后使用本领域已知的方法分选囊泡，以分离显示可能有益的酶功能变化的那些变体。

    Concentration Measuring Method, Concentration Test Instrument, and Concentration Measuring Apparatus
    5.
    发明申请
    Concentration Measuring Method, Concentration Test Instrument, and Concentration Measuring Apparatus 有权
    浓度测量方法，浓度测试仪器和浓度测量仪器

    公开(公告)号：US20040245121A1

    公开(公告)日：2004-12-09

    申请号：US10489488

    申请日：2004-03-12

    发明人： Kenji Nagakawa ,  Hideaki Yamaoka

    IPC分类号： G01N001/00 ,  G01N033/50 ,  C25B013/00 ,  C12N009/00

    CPC分类号： C12Q1/004

    摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.

    摘要翻译： 本发明涉及用于构建包括测试对象，氧化还原酶和电子介体的反应系统的技术，并且通过电化学过程测量测试目标的浓度。 使用Ru化合物作为电子介体。 本发明提供了一种浓度测试仪器，其包括基板，形成在基板上的第一和第二电极以及形成为固体的试剂层。 试剂层含有氧化还原酶和Ru化合物，构成为在供给样品液时溶解并构成液相反应体系。

    Novel cyclodextrin glucanotransferase (cgtase), obtained from<I> bacillus agaradherens</I> (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
    7.
    发明申请
    Novel cyclodextrin glucanotransferase (cgtase), obtained from bacillus agaradherens (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase 有权
    从琼脂杆菌（dsm 9948）获得的新型环糊精葡聚糖转移酶（cgtase）和含有所述新型环糊精葡聚糖转移酶的洗涤剂和清洁剂

    公开(公告)号：US20040235125A1

    公开(公告)日：2004-11-25

    申请号：US10433014

    申请日：2003-05-27

    发明人： Beatrix Kottwitz ,  Karl-Heinz Maurer ,  Roland Breves ,  Irmgard Schmidt ,  Angrit Weber ,  Angela Hellebrandt ,  Laura Polanyi-Bald

    IPC分类号： C12N009/00

    CPC分类号： C12N9/2417 ,  A23K20/189 ,  A23L33/17 ,  A61K8/66 ,  A61Q19/10 ,  C11D3/386 ,  C12N9/1074 ,  D21H25/18

    摘要： The invention relates to a novel enzyme obtained from Bacillus agaradherens (DSM 9948), which can be regarded as cyclodextrin glucanotransferase (CGTase) and also as null-amylase. The invention also relates to sufficiently similar proteins or derivatives that exhibit amylolytic and/or CGTase activity, to the corresponding nucleic acids and micro-organisms that produce said proteins and derivatives, to methods for the production of said proteins and to diverse uses of the same. In addition, said proteins can be further developed for other, predominantly technical purposes. The invention relates in particular to detergents and cleaning agents containing CGTase of this type, to methods for cleaning textiles or hard surfaces using CGTase of this type or corresponding agents and to the use thereof for cleaning textiles or hard surfaces.

    摘要翻译： 本发明涉及从琼脂糖琼脂（DSM 9948）获得的新型酶，其可以被认为是环糊精葡聚糖转移酶（CGTase），也可以作为α-淀粉酶。 本发明还涉及对产生所述蛋白质和衍生物的相应核酸和微生物产生淀粉分解和/或CGT酶活性的足够相似的蛋白质或衍生物，用于生产所述蛋白质的方法及其各种用途 。 此外，所述蛋白质可以进一步开发用于其它主要技术目的。 本发明特别涉及含有这种类型的CGTase的洗涤剂和清洁剂，使用这种类型的CGTase或相应的试剂清洁纺织品或硬表面的方法以及用于清洁纺织品或硬表面的用途。

    Method for the production of protein preparations with essentially constant properties with regard to solubility and functionality within a ph range from about ph 3 to ph 10
    9.
    发明申请
    Method for the production of protein preparations with essentially constant properties with regard to solubility and functionality within a ph range from about ph 3 to ph 10 有权
    制备蛋白质制剂的方法，该溶液和功能在pH范围从约pH3至pH10范围内具有基本恒定的性质

    公开(公告)号：US20040214300A1

    公开(公告)日：2004-10-28

    申请号：US10204489

    申请日：2002-12-11

    发明人： Andreas Wasche ,  Thomas Luck ,  Wolfgang Holley ,  Stefanie Dudek

    IPC分类号： C12N009/00 ,  C07K014/415

    CPC分类号： A23J1/14 ,  A23V2002/00 ,  A23V2300/14 ,  A23V2300/34

    摘要： Disclosed is a method for producing protein preparations having essentially constant functional properties for technical applications within a broad pH range from about pH 3 to pH 10 by means of extraction from a starting product which contains protein, notably legumes, grains or oilseed. The invention is distinguished by the protein-containing starting product undergoing at least one liquid extraction step and that the raffinate and/or extract obtained thereby undergoes at least one membrane separation process in which a residue is obtained or undergoes thermal upgrading in which a concentrate is obtained and that the residue or the concentrate contain the protein preparations having the desired functional properties.

    摘要翻译： 公开了一种通过从含有蛋白质，特别是豆类，谷物或油籽的起始产物的提取，生产在pH范围从约pH 3至pH 10范围内的技术应用基本上具有恒定功能特性的蛋白质制剂的方法。 本发明的特征在于含有起始物的蛋白质经历至少一个液体提取步骤，并且由此得到的萃余液和/或提取物经历至少一个膜分离方法，其中获得残余物或进行热升级，其中浓缩物为 并且残留物或浓缩物含有具有所需功能性质的蛋白质制剂。