公开(公告)号：US11927594B2

公开(公告)日：2024-03-12

申请号：US16785434

申请日：2020-02-07

申请人： Rensselaer Center for Translational Research, Inc.

发明人： Lawrence S. Zisman

IPC分类号： G01N33/68

CPC分类号： G01N33/6827 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2800/12 ,  G01N2800/321

摘要： The present disclosure describes a method of quantifying amounts of phosphopeptides using isotopically-enriched peptides as internal standards. A kit comprising at least one isotopically-enriched phosphorylated peptide can be used to quantify changes in amounts of phosphopeptides using parallel reaction monitoring mass spectrometry techniques. The invention can be used to indicate the pathologic mechanism, severity of the disease, and treatment response of a subject. The invention can also be used to identify subjects who require more aggressive therapeutic interventions or alternative treatments.

公开(公告)号：US20240069035A1

公开(公告)日：2024-02-29

申请号：US18261090

申请日：2022-01-12

申请人： Biological Dynamics, Inc.

发明人： Rajaram KRISHNAN ,  Robert TURNER ,  Juan Pablo HINESTROSA SALAZAR ,  Jean LEWIS ,  Iryna CLARK ,  David SEARSON

IPC分类号： G01N33/68 ,  G01N1/34

CPC分类号： G01N33/6848 ,  G01N1/34 ,  G01N33/6893

摘要： Provided herein are methods and systems for discovering biomarkers associated with risk of disease and methods using identified biomarkers for detecting disease prognosis and progression.

公开(公告)号：US20240069013A1

公开(公告)日：2024-02-29

申请号：US18500793

申请日：2023-11-02

申请人： AmberGen, Inc.

发明人： Mark J. Lim ,  Vladislav B. Bergo ,  Kenneth J. Rothschild

IPC分类号： G01N33/543 ,  G01N33/53 ,  G01N33/68

CPC分类号： G01N33/54306 ,  G01N33/5308 ,  G01N33/6845 ,  G01N33/6848

摘要： Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

公开(公告)号：US20240068006A1

公开(公告)日：2024-02-29

申请号：US18154906

申请日：2023-01-16

申请人： China Medical University

发明人： Der-Yang Cho ,  Po-Ren Hsueh ,  Jiaxin Yu ,  Ni Tien ,  Hsiu Hsien Lin ,  Chia-Fong Cho

IPC分类号： C12Q1/10 ,  G01N33/68

CPC分类号： C12Q1/10 ,  G01N33/6848 ,  G01N2333/986

摘要： A method for assessing drug-resistant Klebsiella pneumoniae includes the following steps. A test sample is provided, wherein the test sample includes a Klebsiella pneumoniae. A spectrum analysis step is performed, wherein the test sample is detected by a mass spectrometry method so as to obtain a target mass spectrum data. An assessing step for drug-resistant Klebsiella pneumoniae is performed, wherein the target mass spectrum data is analyzed so as to assess whether the Klebsiella pneumoniae is resistant to a carbapenem antibiotic or a colistin or not. When the Klebsiella pneumoniae is resistant to the carbapenem antibiotic, the target mass spectrum data includes a first anti-carbapenem feature mark, and when the Klebsiella pneumoniae is resistant to the colistin, the target mass spectrum data includes a first anti-colistin feature mark.

公开(公告)号：US11913957B2

公开(公告)日：2024-02-27

申请号：US15786924

申请日：2017-10-18

申请人： Biodesix, Inc.

发明人： Paul E. Kearney ,  Kenneth C. Fang ,  Xiao-Jun Li ,  Clive Hayward ,  Douglas Spicer

IPC分类号： G01N33/574 ,  G01N33/68 ,  G01N33/53 ,  G16B20/00 ,  G16B40/00

CPC分类号： G01N33/57423 ,  G01N33/6893 ,  G01N33/53 ,  G01N33/6848 ,  G16B20/00 ,  G16B40/00

摘要： Methods are provided for identifying biomarker proteins that exhibit differential expression in subjects with a first lung condition versus healthy subjects or subjects with a second lung condition. Also provided are compositions comprising these biomarker proteins and methods of using these biomarker proteins or panels thereof to diagnose, classify, and monitor various lung conditions. The methods and compositions provided herein may be used to diagnose or classify a subject as having lung cancer or a non-cancerous condition, and to distinguish between different types of cancer (e.g., malignant versus benign, SCLC versus NSCLC).

公开(公告)号：US11913955B2

公开(公告)日：2024-02-27

申请号：US16643381

申请日：2018-08-22

申请人： TOSOH CORPORATION

发明人： Shohei Myoba ,  Norihisa Ohtake

IPC分类号： G01N33/574 ,  G01N33/68

CPC分类号： G01N33/574 ,  G01N33/6848

摘要： The present invention provides a method for detecting cancer in a simple and highly accurate manner, and a reagent that can be used in the method. According to the present invention, cancer (excluding castration-resistant prostate cancer) is detected by measuring the intact growth and differentiation factor (GDF15) propeptide level, the GDF15 propeptide fragment level, or the total of the intact GDF15 propeptide level and the GDF15 propeptide fragment level, in a sample. The above described method for detecting cancer includes a method for detecting one or more selected from the group consisting of stomach cancer, pancreatic cancer, colorectal cancer, lung cancer, breast cancer and esophageal cancer, and a method for distinguishing and detecting non-small cell lung cancer and small cell lung cancer. Further, a reagent for detecting cancer includes an antibody that specifically recognizes GDF15 propeptide.

公开(公告)号：US20240044876A1

公开(公告)日：2024-02-08

申请号：US18128799

申请日：2023-03-30

申请人： Revolution Medicines, Inc.

发明人： Gregory L. VERDINE ,  David W. WHITE ,  David M. ARMISTEAD ,  Deborah J. PALESTRANT ,  Brian Y. CHOW ,  Chris K. VARMA ,  Mathew Edward SOWA

IPC分类号： G01N33/53 ,  C40B30/04 ,  G01N33/68

CPC分类号： G01N33/5308 ,  C40B30/04 ,  G01N33/68 ,  G01N33/6848 ,  G01N2500/04

摘要： Disclosed herein are methods for identifying novel drug candidates.

公开(公告)号：US20240012000A1

公开(公告)日：2024-01-11

申请号：US18040411

申请日：2021-08-10

申请人： ProteomEdge AB

发明人： Fredrik Edfors ,  David Kotol ,  Mathias Uhlén

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N2333/46

摘要： Disclosed are a mixture comprising at least one internal standard protein, a container comprising the mixture, a method for preparing a container with the mixture, a method for determining the amount of a target protein present in a sample, providing a container comprising the mixture, as well as a kit for carrying out the methods.

公开(公告)号：US20240011999A1

公开(公告)日：2024-01-11

申请号：US18023483

申请日：2021-08-27

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： Livia Schiavinato EBERLIN ,  Erik CRESSMAN ,  Alena V. BENSUSSAN ,  John LIN ,  Chunxiao GUO ,  Savitri KRISHNAMURTHY

IPC分类号： G01N33/68 ,  G16H50/20

CPC分类号： G01N33/6848 ,  G16H50/20

摘要： Methods for detecting lung cancer cells, or lung cancer subtypes, by measuring levels of metabolites are provided. Methods of treating identified cancers are likewise provided

公开(公告)号：US20230417759A1

公开(公告)日：2023-12-28

申请号：US18203854

申请日：2023-05-31

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Yuan Mao ,  Andrew Kleinberg ,  Yunlong Zhao ,  Lili Guo

IPC分类号： G01N33/68 ,  G01N30/34 ,  G01N30/72

CPC分类号： G01N33/6848 ,  G01N33/6857 ,  G01N30/34 ,  G01N30/7233

摘要： A method of enhancing a mass spectral signal is disclosed. The method can include contacting a sample to a separation column under conditions that permit sample components to bind to the substrate; applying a first mobile gradient to the separation column, wherein the first mobile phase gradient comprises trifluoroacetic acid (TFA) and a small molecule additive (e.g., an amino acid) or formic acid (FA) and a small molecule additive (e.g., an amino acid); applying a second mobile gradient to the separation column, wherein the second mobile phase gradient comprises TFA in acetonitrile (ACN) and a small molecule additive (e.g., an amino acid) or formic acid (FA) in ACN and a small molecule additive (e.g., an amino acid); and performing mass spectrometric analysis on eluted sample components.