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15 条记录 (耗时 0.017 秒)

    Use of Amino Acids to Enhance Signal in Mass Spectral Analyses
    1.
    发明公开
    Use of Amino Acids to Enhance Signal in Mass Spectral Analyses 审中-公开

    公开(公告)号：US20230417759A1

    公开(公告)日：2023-12-28

    申请号：US18203854

    申请日：2023-05-31

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： Yuan Mao ,  Andrew Kleinberg ,  Yunlong Zhao ,  Lili Guo

    IPC分类号： G01N33/68 ,  G01N30/34 ,  G01N30/72

    CPC分类号： G01N33/6848 ,  G01N33/6857 ,  G01N30/34 ,  G01N30/7233

    摘要： A method of enhancing a mass spectral signal is disclosed. The method can include contacting a sample to a separation column under conditions that permit sample components to bind to the substrate; applying a first mobile gradient to the separation column, wherein the first mobile phase gradient comprises trifluoroacetic acid (TFA) and a small molecule additive (e.g., an amino acid) or formic acid (FA) and a small molecule additive (e.g., an amino acid); applying a second mobile gradient to the separation column, wherein the second mobile phase gradient comprises TFA in acetonitrile (ACN) and a small molecule additive (e.g., an amino acid) or formic acid (FA) in ACN and a small molecule additive (e.g., an amino acid); and performing mass spectrometric analysis on eluted sample components.

    Use of Amino Acids to Enhance Signal in Mass Spectral Analyses
    2.
    发明申请
    Use of Amino Acids to Enhance Signal in Mass Spectral Analyses 有权

    公开(公告)号：US20210223259A1

    公开(公告)日：2021-07-22

    申请号：US17143890

    申请日：2021-01-07

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： Yuan Mao ,  Andrew Kleinberg ,  Yunlong Zhao ,  Lili Guo

    IPC分类号： G01N33/68

    摘要： A method of enhancing a mass spectral signal is disclosed. The method can include contacting a sample to a separation column under conditions that permit sample components to bind to the substrate; applying a first mobile gradient to the separation column, wherein the first mobile phase gradient comprises trifluoroacetic acid (TFA) and a small molecule additive (e.g., an amino acid) or formic acid (FA) and a small molecule additive (e.g., an amino acid); applying a second mobile gradient to the separation column, wherein the second mobile phase gradient comprises TFA in acetonitrile (ACN) and a small molecule additive (e.g., an amino acid) or formic acid (FA) in ACN and a small molecule additive (e.g., an amino acid); and performing mass spectrometric analysis on eluted sample components.

    Tandem Mass Tag Multiplexed Quantitation of Post-Translational Modifications of Proteins
    5.
    发明申请
    Tandem Mass Tag Multiplexed Quantitation of Post-Translational Modifications of Proteins 有权

    公开(公告)号：US20210231676A1

    公开(公告)日：2021-07-29

    申请号：US17159136

    申请日：2021-01-26

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： Yuan Mao ,  Andrew Kleinberg

    IPC分类号： G01N33/68 ,  G01N30/72

    摘要： Disclosed are methods of quantifying multiple quality attributes, such as post translational modifications, of multiple samples in a single mass spectrometry (MS) run, including contacting two or more samples with a digesting solution under conditions sufficient to digest samples, wherein each sample is digested separately and the digesting solution is a Tris-free buffer solution; contacting each of the two or more digested samples with a specific Tandem Mass Tag (TMT) labeling reagent under conditions sufficient to label peptides within each of the digested samples with the specific TMT labeling reagent; quenching labeling of peptides within each of the two or more digested samples; combining equal volumes of the two or more labeled, digested samples into a single combined sample solution; and analyzing the single combined sample solution by targeted mass spectral analysis, thereby allowing multiple quality attributes of the two or more samples to be quantified in a single mass spectrometry (MS) run.

    Tandem Mass Tag Multiplexed Quantitation of Post-Translational Modifications of Proteins
    6.
    发明公开
    Tandem Mass Tag Multiplexed Quantitation of Post-Translational Modifications of Proteins 审中-公开

    公开(公告)号：US20230349916A1

    公开(公告)日：2023-11-02

    申请号：US18123753

    申请日：2023-03-20

    申请人： REGENERON PHARMACEUTICALS, INC.

    发明人： Yuan Mao ,  Andrew Kleinberg

    IPC分类号： G01N33/68 ,  G01N30/72

    CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N2030/027

    摘要： Disclosed are methods of quantifying multiple quality attributes, such as post translational modifications, of multiple samples in a single mass spectrometry (MS) run, including contacting two or more samples with a digesting solution under conditions sufficient to digest samples, wherein each sample is digested separately and the digesting solution is a Tris-free buffer solution; contacting each of the two or more digested samples with a specific Tandem Mass Tag (TMT) labeling reagent under conditions sufficient to label peptides within each of the digested samples with the specific TMT labeling reagent; quenching labeling of peptides within each of the two or more digested samples; combining equal volumes of the two or more labeled, digested samples into a single combined sample solution; and analyzing the single combined sample solution by targeted mass spectral analysis, thereby allowing multiple quality attributes of the two or more samples to be quantified in a single mass spectrometry (MS) run.

    METHODS FOR DE NOVO PROTEIN SEQUENCING
    7.
    发明申请
    METHODS FOR DE NOVO PROTEIN SEQUENCING 有权

    公开(公告)号：US20210278414A1

    公开(公告)日：2021-09-09

    申请号：US17327889

    申请日：2021-05-24

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： Yuan Mao

    IPC分类号： G01N33/68 ,  G16B40/10 ,  G16B20/00 ,  C07K16/06 ,  G01N27/622 ,  H01J49/30

    摘要： A method for determining an amino acid sequence of a polypeptide, including comprising: contacting a first sample containing the polypeptide with a first protease (e.g., Trypsin) to produce a first set of digested peptide fragments; fragmenting the first set of digested peptide fragments to produce a first set of fragmented peptide ions; determining masses of the first set of fragmented peptide ions; contacting a second sample containing the polypeptide with a second protease (e.g., Tryp-N); fragmenting the second set of digested peptide fragments to produce a second set of fragmented peptide ions; selecting pairs of peptide ions from the first and the second set of fragmented peptide ions that differ in mass by a mass of an arginine amino acid residue or a lysine amino acid residue; assigning an ion type (either N-terminal peptide ion or C-terminal peptide ion) to the selected pairs of the peptide ions from two sets of fragmented peptide ions; selecting a mass ladder of the same-type peptide ions in either set of fragmented peptide ions with incremental mass by the mass of amino acid residue(s), and assembling the identified amino acid residues from the mass ladder to determine the amino acid sequence of the polypeptide of interest.

    Methods for de novo protein sequencing
    8.
    发明授权
    Methods for de novo protein sequencing 有权

    公开(公告)号：US12000840B2

    公开(公告)日：2024-06-04

    申请号：US17327889

    申请日：2021-05-24

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： Yuan Mao

    IPC分类号： G01N33/68 ,  C07K16/06 ,  G16B20/00 ,  G16B40/10 ,  H01J49/30

    CPC分类号： G01N33/6851 ,  C07K16/065 ,  G16B20/00 ,  G16B40/10 ,  H01J49/305

    摘要： A method for determining an amino acid sequence of a polypeptide, including comprising: contacting a first sample containing the polypeptide with a first protease (e.g., Trypsin) to produce a first set of digested peptide fragments; fragmenting the first set of digested peptide fragments to produce a first set of fragmented peptide ions; determining masses of the first set of fragmented peptide ions; contacting a second sample containing the polypeptide with a second protease (e.g., Tryp-N); fragmenting the second set of digested peptide fragments to produce a second set of fragmented peptide ions; selecting pairs of peptide ions from the first and the second set of fragmented peptide ions that differ in mass by a mass of an arginine amino acid residue or a lysine amino acid residue; assigning an ion type (either N-terminal peptide ion or C-terminal peptide ion) to the selected pairs of the peptide ions from two sets of fragmented peptide ions; selecting a mass ladder of the same-type peptide ions in either set of fragmented peptide ions with incremental mass by the mass of amino acid residue(s), and assembling the identified amino acid residues from the mass ladder to determine the amino acid sequence of the polypeptide of interest.