公开(公告)号：US20220187259A1

公开(公告)日：2022-06-16

申请号：US17593109

申请日：2020-06-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Lyle Lorrence Burton ,  Stephen Alexander Tate

IPC: G01N30/86 ,  G01N30/72 ,  G01N30/14

Abstract: A separation device is instructed to separate a compound from a sample over a time period. A mass spectrometer is instructed to measure a plurality of intensities of at least one ion of the separated compound over the time period, producing a chromatogram. At least one peak of the at least one ion is identified from the chromatogram using a peak-finding algorithm. Two or more different peak integration areas are calculated for the at least one peak by applying the peak-finding algorithm with two or more different values for at least one peak-finding parameter. Two or more plots of the at least one peak that each shows graphically a different peak integration area are displayed on a display device at the same time. In response, data is received from a user selection device that indicates user selection of one of the two or more plots.

公开(公告)号：US20220181132A1

公开(公告)日：2022-06-09

申请号：US17457055

申请日：2021-12-01

Applicant: DH Technologies Development Pte. Ltd. ,  ETH Zürich

Inventor： Ronald F. Bonner ,  Stephen A. Tate ,  Rudolf Aebersold ,  Pedro Jose Navarro Alvarez ,  Oliver Rinner ,  Lukas Reiter ,  Ludovic Gillet

IPC: H01J49/00 ,  G16C20/90 ,  H01J49/42 ,  G01N30/72

Abstract: Systems and methods are disclosed for analyzing a sample using overlapping precursor isolation windows. A mass analyzer of a tandem mass spectrometer is instructed to select and fragment at least two overlapping precursor isolation windows across a precursor ion mass range of a sample using a processor. The tandem mass spectrometer includes a mass analyzer that allows overlapping precursor isolation windows across the mass range of the sample.

公开(公告)号：US11355334B2

公开(公告)日：2022-06-07

申请号：US16312009

申请日：2017-06-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/06 ,  H01J49/00

Abstract: Methods and systems are provided herein for selectively removing product ions resulting from an ECD dissociation event from the interaction region of an ECD reaction cell, while other precursor peptide ions continue to undergo ECD within the interaction region, thereby reducing or preventing the occurrence of multiple electron capture events by the product ions. In some aspects, the preferential extraction of product ions from the interaction region during the ECD reaction can occur without an auxiliary AC field being generated within the interaction region. Additionally, in some aspects, the methods and systems disclosed herein can subject the various product ions to a non-dissociative charge reduction via exposure to reagent ions of the opposite polarity so as to selectively concentrate product ions to a lower charge state.

公开(公告)号：US20220139690A1

公开(公告)日：2022-05-05

申请号：US17427428

申请日：2020-02-03

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Thomas R. Covey ,  Chang Liu ,  Stephen A Tate

IPC: H01J49/16 ,  H01J49/04 ,  G01N30/72

Abstract: A device that produces charged droplets whose composition is optimized for the creation of ions by electro spray composed of: a transport device that is operative to transfer sample components from a liquid sample to a processing chamber, a flowing stream of liquid through the processing chamber into which the samples are deposited, a controller mechanism operative to control the amount of sample transferred, a transport tube through which the flowing liquid containing the sample is directed to an electro spray emitter with a high electric field at the exit, a flow of expanding gas surrounding the electro spray emitter creating a pressure drop at the exit, and, a mass spectrometer for measuring the number of ions produced from the charged droplets emanating from the emitter; wherein the dilution of the sample in the processing chamber and transport fluid is from 100 to 10,000-fold.

公开(公告)号：US20220102133A1

公开(公告)日：2022-03-31

申请号：US17427386

申请日：2020-01-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Eric T Dziekonski

IPC: H01J49/42 ,  H01J49/38

Abstract: In one aspect, a mass analyzer is disclosed, which comprises a quadrupole having an input end for receiving ions and an output end through which ions can exit the quadrupole, said quadrupole having a plurality of rods to at least some of which a drive RF signal and an excitation signal can be applied. A fixed phase relationship is maintained between the drive RF signal and the excitation signal, thereby enhancing the signal-to-noise ratio of the mass detection signal.

公开(公告)号：US20220013348A1

公开(公告)日：2022-01-13

申请号：US17312902

申请日：2019-12-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Eric Thomas Dziekonski

IPC: H01J49/40 ,  H01J49/02 ,  H01J49/42 ,  H01J49/06 ,  H01J49/16

Abstract: An MCP detector (620) receives an ion packet along an ion path (601) of mass spectrometer through a hollow central cylindrical tube (621) of the MCP detector. The MCP detector includes coaxial rings (622) of MCPs surrounding the hollow central cylindrical tube. The MCP detector transmits the ion packet along the ion path to an ELIT (610) through holes in the center of a first set of reflectron plates (613) of the ELIT to oscillate the ion packet between the first set and a second set of reflectron plates (614) of the ELIT. The ELIT transmits the oscillated ion packet back to the MCP detector along the ion path through the holes of the first set. The MCP detector detects ions of the oscillated ion packet that are radially deflected from the ion path using the rings of MCPs. The MCP detector allows ions to be transmitted to or from either port of the ELIT.

公开(公告)号：US20210384023A1

公开(公告)日：2021-12-09

申请号：US17410758

申请日：2021-08-24

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/28 ,  G01N29/24 ,  G01N24/00 ,  G01N1/38 ,  G01N29/22 ,  G01N29/02 ,  G01N35/10 ,  B05B17/06

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20210351026A1

公开(公告)日：2021-11-11

申请号：US17284336

申请日：2019-10-08

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi BABA ,  Pavel RYUMIN

IPC: H01J49/00 ,  H01J49/02

Abstract: In one aspect, an electron-ion reaction module, e.g., an electron capture dissociation module, for use in a mass spectrometer is disclosed, which comprises a chamber, an electron source for generating electrons and introducing the electrons into the chamber, a gate electrode positioned relative to the electron source and the chamber, and a DC voltage source operatively coupled to the gate electrode for applying control voltages to the gate electrode. The electron-ion interaction module can further include a controller operably coupled to the DC voltage source and configured for adjusting the DC voltage applied to the gate electrode to adjust flow of electrons into the chamber.

公开(公告)号：US11145503B2

公开(公告)日：2021-10-12

申请号：US16981057

申请日：2019-05-23

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Eric Thomas Dziekonski

IPC: H01J49/00 ,  H01J49/02 ,  H01J49/42

Abstract: A mass spectrometer is operated to simultaneously measure precursor and production data over a number of acquisitions. For each acquisition, the following steps are performed. Ion transfer optics inject ions from an ion beam into an ELIT causing the ions to oscillate axially between two electric fields produced by two the sets of reflectrons. The ELIT measures a time domain image current of the oscillating ions from ion injection to a total acquisition time, Tacq1, and fragments the oscillating ions at one or both turning points of the oscillating ions adding product ions to the oscillating ions. The fragmentation is performed at a delay time relative to the ion injection that is increased by a time increment in each subsequent acquisition making the fragmentation dependent on ion position. The measured time domain image current is stored as a row or column of a two-dimensional matrix.

公开(公告)号：US20210265152A1

公开(公告)日：2021-08-26

申请号：US17260734

申请日：2019-07-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W. Arnold

IPC: H01J49/04

Abstract: Integrated system for delivering sample to a mass spectrometer, which includes a chamber extending from a top to bottom end, an open port probe disposed in the chamber such that an open end of the probe, which is configured for receiving a sample, is positioned in proximity to top end of the chamber. The system can further include a solvent inlet port coupled to said chamber for receiving a solvent and directing said solvent to said probe, and a solvent outlet port for receiving a flow of the solvent from the open port probe and directing the received solvent out of the chamber. The system can also include an adapter for receiving a sample holder having an outlet port, the adapter is releasable and replaceable and couple with chamber to align the outlet port of sample holder with open end of probe for delivering sample to the probe.