利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

98 条记录 (耗时 0.029 秒)

    Amorphous solid succinylated 3-(fatty acid amido)-2-hydroxy-1 -(protected hydroxy)-propane salts and methods of making the same
    51.
    发明授权
    Amorphous solid succinylated 3-(fatty acid amido)-2-hydroxy-1 -(protected hydroxy)-propane salts and methods of making the same 有权

    公开(公告)号：US11530179B2

    公开(公告)日：2022-12-20

    申请号：US17079129

    申请日：2020-10-23

    申请人： Geron Corporation

    发明人： Jennifer E. Albaneze-Walker

    IPC分类号： C07C235/00 ,  C07C231/12

    摘要： Aspects of the disclosure includes methods for preparing an amorphous solid composition of a fatty acid metal salt. In practicing the subject methods according to certain embodiments, a succinylated 3-(fatty acid amido)-2-hydroxy-1-(protected hydroxy)-propane organic salt is contacted with a metal base to produce a succinylated 3-(fatty acid amido)-2-hydroxy-1-(protected hydroxy)-propane metal salt; and the succinylated 3-(fatty acid amido)-2-hydroxy-1-(protected hydroxy)-propane metal salt is precipitated in a solvent to produce an amorphous solid succinylated 3-(fatty acid amido)-2-hydroxy-1-(protected hydroxy)-propane metal salt composition. An amorphous solid succinylated 3-(fatty acid amido)-2-hydroxy-1-(protected hydroxy)-propane lithium salt is also provided.

    METHODS OF POLYNUCLEOTIDE PREPARATION USING MULTIVALENT CATION SALT COMPOSITIONS
    55.
    发明申请
    METHODS OF POLYNUCLEOTIDE PREPARATION USING MULTIVALENT CATION SALT COMPOSITIONS 审中-公开

    公开(公告)号：US20200339975A1

    公开(公告)日：2020-10-29

    申请号：US16926340

    申请日：2020-07-10

    申请人： Geron Corporation

    发明人： Premchandran H. Ramiya

    IPC分类号： C12N15/10 ,  C12N15/113

    摘要： Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes contacting a first polynucleotide composition including: a polynucleotide having a sequence of 7 or more nucleoside subunits and at least two of the nucleoside subunits are joined by a N3′→P5′ thiophosphoramidate inter-subunit linkage; and non-target synthetic products and reagents; with a multivalent cation salt to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt. In certain embodiments, the method further includes contacting the polynucleotide salt with a reverse phase chromatography support; and eluting from the chromatography support a third polynucleotide composition including the polynucleotide. Also provided are compositions including a salt of the polynucleotide including at least one multivalent cation counterion.

    Methods of polynucleotide preparation using multivalent cation salt compositions
    57.
    发明授权
    Methods of polynucleotide preparation using multivalent cation salt compositions 有权

    公开(公告)号：US10745687B2

    公开(公告)日：2020-08-18

    申请号：US15134740

    申请日：2016-04-21

    申请人： Geron Corporation

    发明人： Premchandran H. Ramiya

    IPC分类号： C12N15/10 ,  C12N15/113

    摘要： Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes contacting a first polynucleotide composition including: a polynucleotide having a sequence of 7 or more nucleoside subunits and at least two of the nucleoside subunits are joined by a N3′→P5′ thiophosphoramidate inter-subunit linkage; and non-target synthetic products and reagents; with a multivalent cation salt to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt. In certain embodiments, the method further includes contacting the polynucleotide salt with a reverse phase chromatography support; and eluting from the chromatography support a third polynucleotide composition including the polynucleotide. Also provided are compositions including a salt of the polynucleotide including at least one multivalent cation counterion.

    Oligonucleotide compositions and methods of making the same
    60.
    发明授权
    Oligonucleotide compositions and methods of making the same 有权

    公开(公告)号：US10392418B2

    公开(公告)日：2019-08-27

    申请号：US15705019

    申请日：2017-09-14

    申请人： Geron Corporation

    发明人： Premchandran H. Ramiya

    IPC分类号： C07H21/00 ,  C12N15/113 ,  C12P19/34

    摘要： The present disclosure provides a solid phase method of making oligonucleotides via sequential coupling cycles including at least one coupling of a dinucleotide dimer subunit to a free 3′-terminal group of a growing chain. The oligonucleotides include at least two nucleoside subunits joined by a N3′→P5′ phosphoramidate linkage. The method may include the steps of (a) deprotecting the protected 3′ amino group of a terminal nucleoside attached to a solid phase support, said deprotecting forming a free 3′ amino group; (b) contacting the free 3′ amino group with a 3′-protected amino-dinucleotide-5′-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an internucleoside N3′→P5′ phosphoramidite linkage; and (c) oxidizing (e.g., sulfurizing) the linkage. The compositions produced by the subject methods may include a reduced amount of one or more (N−x) oligonucleotide products. Also provided are pharmaceutical compositions including the subject oligonucleotide compositions.