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    Method of producing fumaric acid
    51.
    发明授权
    Method of producing fumaric acid 失效
    产生富马酸的方法

    公开(公告)号：US5783428A

    公开(公告)日：1998-07-21

    申请号：US804144

    申请日：1997-02-20

    申请人： Makoto Goto ,  Terukazu Nara ,  Izuru Tokumaru ,  Nobutake Fugono ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa

    发明人： Makoto Goto ,  Terukazu Nara ,  Izuru Tokumaru ,  Nobutake Fugono ,  Yasukazu Uchida ,  Masato Terasawa ,  Hideaki Yukawa

    IPC分类号： C12P7/46 ,  C12P13/20

    CPC分类号： C12P7/46 ,  C12P13/20 ,  Y10S435/832 ,  Y10S435/833

    摘要： Fumaric acid is produced by reacting a culture of a microorganism which produces maleate isomerase that exhibits a maximum activity at not less than 50.degree. C. or a treated product thereof with maleic acid in an aqueous solution, and isomerizing maleic acid to produce fumaric acid. L-aspartic acid is produced by reacting both of a culture of a microorganism which produces maleate isomerase or a treated product thereof and a culture of a microorganism which produces aspartase or a treated product thereof with maleic acid and ammonia in an aqueous solution, producing L-aspartic acid from maleic acid and ammonia by enzyme reactions, and recovering L-aspartic acid from the reaction mixture.

    摘要翻译： 富马酸是通过使生成马来酸异构酶的微生物培养物（其在50℃以上表现出最大活性）或其处理产物与马来酸在水溶液中反应，并使马来酸异构化以产生富马酸而制备的。 L-天冬氨酸通过使水解产生马来酸异构酶的微生物培养物或其处理产物和产生天冬氨酰胺的微生物培养物或其处理产物与马来酸和氨反应，生成L - 天冬氨酸通过酶反应从马来酸和氨中回收，并从反应混合物中回收L-天冬氨酸。

    Method for producing fumaric acid
    52.
    发明授权
    Method for producing fumaric acid 失效
    富马酸的生产方法

    公开(公告)号：US5677156A

    公开(公告)日：1997-10-14

    申请号：US623001

    申请日：1996-03-28

    申请人： Makoto Goto ,  Izuru Tokumaru ,  Masato Terasawa ,  Hideaki Yukawa

    发明人： Makoto Goto ,  Izuru Tokumaru ,  Masato Terasawa ,  Hideaki Yukawa

    IPC分类号： C12P7/46 ,  C12P13/20 ,  C12N9/90

    CPC分类号： C12P7/46 ,  C12P13/20 ,  Y10S435/829 ,  Y10S435/84

    摘要： Fumaric acid is produced by reacting maleic acid in an aqueous solution with a microorganism which has maleate isomerase activity or with a preparation from the microorganism having the maleate isomerase activity, and producing fumaric acid in a reaction solution by enzymatic isomerization of maleic acid carried out under the condition that the dissolved oxygen concentration in the reaction solution is substantially maintained at 4 ppm or less, for example, by sealing the reaction solution with one or more gases selected from N.sub.2, Ar, and He.

    摘要翻译： 通过马来酸在水溶液中与具有马来酸异构酶活性的微生物或具有马来酸异构酶活性的微生物的制剂反应制备富马酸，并通过在下面进行的马来酸的酶促异构化在反应溶液中产生富马酸 反应溶液中的溶解氧浓度基本上保持在4ppm以下的条件，例如通过用一种或多种选自N 2，Ar和He的气体密封反应溶液。

    Wire cutting type electrical discharge machining system
    55.
    发明授权
    Wire cutting type electrical discharge machining system 失效
    线切割式放电加工系统

    公开(公告)号：US4689462A

    公开(公告)日：1987-08-25

    申请号：US705532

    申请日：1985-02-26

    申请人： Makoto Goto

    发明人： Makoto Goto

    IPC分类号： B23H7/02 ,  B23H1/02 ,  B23H7/04

    CPC分类号： B23H7/02

    摘要： A wire cutting type electrical discharge machining system includes a wire supply device, an upper and lower electrode guides, and a wire take-up device for feeding a wire electrode so as to carry out the discharge machining on an electrically conductive workpiece. An improved wire-disconnection detecting device attached to the system includes a first and second wire-slack detecting members of metallic tube through which the wire is passed with a small distance formed therebetween, a wire-disconnection detecting circuits connected between the detecting members and the wire, including a power supply, and a sensor for sensing a flow of current from the power supply through the detecting members and the wire upon contact of the two due to a slack of the wire. A disconnection of the wire is detected when the flow of current is sensed by the sensor.

    摘要翻译： 线切割型放电加工系统包括供电装置，上下电极引导件和用于馈送线电极的线取出装置，以在导电工件上进行放电加工。 安装在该系统上的改进的断线检测装置包括金属管的第一和第二线松弛检测部件，金属管通过该第一和第二线松弛检测部件，导线沿其间形成小的距离而通过，断线检测电路连接在检测部件和 线，包括电源，以及传感器，用于在由于电线松弛而接触到两者时，通过检测部件和电线感测来自电源的电流。 当传感器检测到电流时，检测到电线断线。