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公开(公告)号：US20220187182A1
公开(公告)日：2022-06-16
申请号：US17588040
申请日：2022-01-28
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Eric D. Diebold , Jonathan Lin , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US11227672B2
公开(公告)日：2022-01-18
申请号：US16596428
申请日：2019-10-08
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US20210333192A1
公开(公告)日：2021-10-28
申请号：US17226681
申请日：2021-04-09
Applicant: Becton, Dickinson and Company
Inventor： David Alexander Roberts , Lingjie Wei , Peter Mage , Keegan Owsley
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.
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公开(公告)号：US20200333236A1
公开(公告)日：2020-10-22
申请号：US16803164
申请日：2020-02-27
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , David A. Roberts
IPC: G01N15/14
Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.
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公开(公告)号：US20200209140A1
公开(公告)日：2020-07-02
申请号：US16814394
申请日：2020-03-10
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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Patent Agency Ranking
公开(公告)号：US20200209064A1
公开(公告)日：2020-07-02
申请号：US16725799
申请日：2019-12-23
Applicant: Becton, Dickinson and Company
Inventor： Keegan Owsley , Peter Mage
Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.
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公开(公告)号：US20180275042A1
公开(公告)日：2018-09-27
申请号：US15992097
申请日：2018-05-29
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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公开(公告)号：US20240369411A1
公开(公告)日：2024-11-07
申请号：US18734968
申请日：2024-06-05
Applicant: Becton, Dickinson and Company
Inventor： Peter Mage , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.
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公开(公告)号：US12105010B2
公开(公告)日：2024-10-01
申请号：US18373487
申请日：2023-09-27
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14 , G01N15/1429 , G01N15/1434 , G01N15/149 , G01N15/1404
CPC classification number: G01N15/1459 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N2015/1402 , G01N2015/142 , G01N2015/1486 , G01N15/149
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US12078587B2
公开(公告)日：2024-09-03
申请号：US17953976
申请日：2022-09-27
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
IPC: G01N15/14 , G01N15/1429 , G01N15/1434 , G01N21/64 , G01N15/10 , G01N15/149
CPC classification number: G01N15/1434 , G01N15/1429 , G01N15/147 , G01N21/6402 , G01N21/6428 , G01N2015/1006 , G01N2015/1447 , G01N15/149 , G01N2021/6439
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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