公开(公告)号：US20220390349A1

公开(公告)日：2022-12-08

申请号：US17699865

申请日：2022-03-21

Applicant: Becton, Dickinson and Company

Inventor： David Alexander Roberts ,  Christopher J. Wolf ,  Keegan Owsley

IPC: G01N15/14 ,  G01N33/49 ,  G01N33/493

Abstract: Methods of classifying flow cytometer data are provided. Methods of interest include receiving a first gate and flow cytometer data, expanding the first gate to generate a second gate, and determining sets of flow cytometer data encompassed by each of the first gate and the second gate to classify the flow cytometer data. In embodiments, methods also involve recording a subset of the classified flow cytometer data and optionally adjusting the first and/or second gates based on the recorded data. In some cases, the subject methods include sorting particles associated with the classified flow cytometer data based on the first and second gates. Systems and computer-readable storage media for practicing the invention are also provided.

公开(公告)号：US20250164374A1

公开(公告)日：2025-05-22

申请号：US19030471

申请日：2025-01-17

Applicant: Becton, Dickinson and Company

Inventor： Peter Ludington Mage ,  Lingjie Wei ,  Peter Johnson ,  David Alexander Roberts ,  Keegan O. Owsley

IPC: G01N15/10 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/149 ,  G01N21/64

Abstract: Aspects of the present disclosure include methods for determining baseline noise of a photodetector (e.g., in a light detection system of a particle analyzer). Methods according to certain embodiments include irradiating a sample having particles in a flow stream, detecting light with the photodetector from the irradiated flow stream, generating data signals from the detected light and calculating a moving average mean squared error of the generated data signals to determine the baseline of the photodetector. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium are also provided.

公开(公告)号：US11662297B2

公开(公告)日：2023-05-30

申请号：US17226681

申请日：2021-04-09

Applicant: Becton, Dickinson and Company

Inventor： David Alexander Roberts ,  Lingjie Wei ,  Peter Mage ,  Keegan Owsley

IPC: G01N15/14

CPC classification number: G01N15/147 ,  G01N15/1434 ,  G01N2015/1447 ,  G01N2015/1493

Abstract: Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.

公开(公告)号：US20210333192A1

公开(公告)日：2021-10-28

申请号：US17226681

申请日：2021-04-09

Applicant: Becton, Dickinson and Company

Inventor： David Alexander Roberts ,  Lingjie Wei ,  Peter Mage ,  Keegan Owsley

IPC: G01N15/14

Abstract: Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.